Detailed information    

experimental Experimentally validated

Overview

Name   ruvB   Type   Machinery gene
Locus tag   C694_RS05445 Genome accession   NC_018939
Coordinates   1120739..1121749 (+) Length   336 a.a.
NCBI ID   WP_000664449.1    Uniprot ID   O25699
Organism   Helicobacter pylori 26695     
Function   homologous recombination   
Homologous recombination

Function

Inactivation of recA, recB, recN, ruvA, ruvB, and ruvC significantly reduced the number of recombinant clones.


Genomic Context

Location: 1115739..1126749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C694_RS05420 (C694_05450) - 1115939..1117261 (-) 1323 WP_000423121.1 M23 family metallopeptidase -
  C694_RS05425 (C694_05455) - 1117271..1118215 (-) 945 WP_000911251.1 outer membrane beta-barrel protein -
  C694_RS05430 (C694_05460) - 1118224..1119066 (-) 843 WP_001862786.1 outer membrane beta-barrel protein -
  C694_RS05435 (C694_05465) - 1119056..1119778 (-) 723 WP_001862785.1 hypothetical protein -
  C694_RS05440 (C694_05470) panB 1119927..1120739 (+) 813 WP_000062669.1 3-methyl-2-oxobutanoate hydroxymethyltransferase -
  C694_RS05445 (C694_05475) ruvB 1120739..1121749 (+) 1011 WP_000664449.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  C694_RS05450 (C694_05480) tatB 1121817..1122299 (+) 483 WP_000467616.1 Sec-independent protein translocase protein TatB -
  C694_RS05455 (C694_05485) tatC 1122292..1123053 (+) 762 WP_000461250.1 twin-arginine translocase subunit TatC -
  C694_RS05460 (C694_05490) queA 1123054..1124091 (+) 1038 WP_000657423.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  C694_RS05465 (C694_05495) rsmG 1124088..1124624 (+) 537 WP_001068828.1 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG -
  C694_RS05470 (C694_05500) - 1124618..1124848 (+) 231 WP_000944436.1 PP0621 family protein -
  C694_RS05475 (C694_05505) - 1124851..1125258 (+) 408 WP_000906037.1 hypothetical protein -
  C694_RS05485 (C694_05510) - 1125391..1125963 (-) 573 WP_001879972.1 outer membrane protein -
  C694_RS05490 (C694_05515) - 1126284..1126658 (+) 375 WP_000772151.1 chemotaxis response regulator CheY -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37360.42 Da        Isoelectric Point: 6.0715

>NTDB_id=1489 C694_RS05445 WP_000664449.1 1120739..1121749(+) (ruvB) [Helicobacter pylori 26695]
MKERIVNLETLDFEISQEVSLRPSLWEDFIGQEKIKSNLQISICAAKKRQESLDHMLFFGPPGLGKTSISHIIAKEMETN
IKITAAPMIEKSGDLAAILTNLQAKDILFIDEIHRLSPAIEEVLYPAMEDFRLDIIIGSGPAAQTIKIDLPPFTLIGATT
RAGMLSNPLRDRFGMSFRMQFYNPSELALIIKKAAVKLNQDIKQESADEIAKRSRGTPRIALRLLKRVRDFALVKNSSLM
DLNITLHALNELGVNELGFDEADLAYLSLLANAQGKPVGLNTIAASMREDEGTIEDVIEPFLLANGYLERTAKGRIATPK
THELLKIPTLNPQTLF

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=1489 C694_RS05445 WP_000664449.1 1120739..1121749(+) (ruvB) [Helicobacter pylori 26695]
ATGAAAGAACGGATAGTCAATTTAGAAACTTTGGATTTTGAAATTTCTCAAGAAGTGAGTTTGCGCCCTAGTCTTTGGGA
AGATTTTATCGGTCAAGAAAAGATTAAAAGCAATTTGCAAATTTCTATTTGCGCGGCTAAAAAACGCCAAGAAAGTTTGG
ATCACATGCTTTTTTTTGGCCCGCCCGGTTTGGGTAAAACTTCAATCAGCCATATCATCGCTAAAGAAATGGAAACCAAT
ATCAAGATCACCGCCGCTCCCATGATAGAAAAAAGCGGTGATTTAGCCGCCATTTTGACCAATTTGCAAGCTAAAGACAT
TCTTTTTATTGATGAAATCCACCGGCTCAGCCCAGCGATTGAAGAGGTTTTATACCCGGCGATGGAAGATTTTAGGTTGG
ATATTATCATAGGCTCAGGCCCAGCGGCTCAAACCATTAAAATTGATTTACCCCCTTTCACTCTCATCGGCGCTACCACC
AGAGCCGGAATGCTCTCTAACCCCTTAAGAGACAGATTTGGCATGAGTTTTAGAATGCAATTTTATAACCCTAGCGAACT
GGCCCTCATCATTAAAAAAGCTGCCGTTAAACTCAACCAAGACATCAAACAAGAAAGTGCTGATGAAATCGCTAAAAGGA
GTAGAGGCACGCCAAGGATCGCTTTAAGGCTTTTAAAAAGGGTGCGCGATTTTGCGCTAGTCAAAAATTCAAGCTTGATG
GATTTAAACATCACTTTGCATGCTTTGAATGAATTAGGCGTGAATGAATTAGGCTTTGATGAAGCGGATTTGGCGTATTT
ATCTTTGTTGGCTAACGCTCAAGGAAAGCCGGTGGGTTTGAACACGATTGCAGCATCTATGAGAGAAGATGAAGGCACGA
TTGAAGACGTGATTGAGCCTTTTTTACTCGCTAATGGTTATTTAGAGCGCACCGCTAAAGGCAGAATCGCCACGCCTAAA
ACCCATGAGCTCTTAAAAATCCCCACTTTAAACCCCCAAACTTTATTTTAA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(256-326)

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O25699

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

52.744

98.204

0.518
  ruvB Streptococcus pneumoniae D39

50.774

97.289

0.494
  ruvB Streptococcus pneumoniae R6

50.774

97.289

0.494
  ruvB Streptococcus pneumoniae TIGR4

50.774

97.289

0.494
  ruvB Synechocystis sp. PCC 6803

51.104

94.345

0.482

References

[1] Stefan Kulick et al. (2008) Mosaic DNA imports with interspersions of recipient sequence after natural transformation of Helicobacter pylori. PloS One 3(11):e3797. [PMID: 19030104]