Detailed information    

experimental Experimentally validated

Overview

Name   ruvB   Type   Machinery gene
Locus tag   SPD_RS01315 Genome accession   NC_008533
Coordinates   232129..233127 (+) Length   332 a.a.
NCBI ID   WP_001809468.1    Uniprot ID   Q8CWU7
Organism   Streptococcus pneumoniae D39     
Function   homologous recombination   
Homologous recombination

Function

The RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR). RuvA specifically binds to HJ cruciform DNA, conferring on it an open structure. The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex.


Genomic Context

Location: 227129..238127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPD_RS01295 (SPD_0238) leuS 227270..229771 (+) 2502 WP_000011789.1 leucine--tRNA ligase -
  SPD_RS01300 (SPD_0239) - 229965..230660 (+) 696 WP_001144910.1 GNAT family N-acetyltransferase -
  SPD_RS01305 (SPD_0240) - 230672..231088 (+) 417 WP_000628392.1 GNAT family N-acetyltransferase -
  SPD_RS01310 - 231903..231995 (-) 93 WP_000970381.1 type I toxin-antitoxin system Fst family toxin -
  SPD_RS01315 (SPD_0241) ruvB 232129..233127 (+) 999 WP_001809468.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  SPD_RS01320 (SPD_0242) - 233108..233680 (+) 573 WP_001098896.1 nucleotidyltransferase family protein -
  SPD_RS01325 (SPD_0243) - 233911..234669 (+) 759 WP_000466719.1 isoprenyl transferase -
  SPD_RS01330 (SPD_0244) - 234678..235481 (+) 804 WP_000189869.1 phosphatidate cytidylyltransferase -
  SPD_RS01335 (SPD_0245) eeP 235503..236762 (+) 1260 WP_000900660.1 RIP metalloprotease RseP Regulator

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 37288.73 Da        Isoelectric Point: 4.5053

>NTDB_id=683 SPD_RS01315 WP_001809468.1 232129..233127(+) (ruvB) [Streptococcus pneumoniae D39]
MSRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPVIEKAGDLVAILNELEPGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRVRDFAQIMGNGVID
DIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAK
AYEHLGYEYSEK

Nucleotide


Download         Length: 999 bp        

>NTDB_id=683 SPD_RS01315 WP_001809468.1 232129..233127(+) (ruvB) [Streptococcus pneumoniae D39]
ATGAGTAGAATTTTAGATAATGAGATAATGGGGGATGAGGAGTTAGTAGAACGCACGCTCCGTCCTCAGTATTTACGTGA
ATATATCGGACAGGATAAGGTCAAGGACCAGCTACAAATCTTTATTGAAGCTGCCAAAATGCGGGATGAAGCGCTGGATC
ATGTGCTCTTATTTGGGCCTCCAGGTCTCGGGAAAACGACCATGGCCTTTGTTATTGCCAACGAACTGGGAGTCAATCTT
AAGCAGACGTCGGGTCCAGTCATTGAAAAAGCCGGAGATCTGGTAGCTATTTTGAATGAGTTAGAGCCTGGGGATGTCCT
TTTTATTGATGAGATCCATCGTTTGCCAATGTCAGTGGAAGAGGTGCTTTATAGTGCTATGGAGGACTTCTACATAGATA
TTATGATTGGGGCTGGTGAGGGTAGTCGTAGTGTTCATTTGGAGTTACCACCTTTTACCTTGATTGGTGCGACGACTCGG
GCTGGTATGCTCTCCAATCCGCTACGGGCACGTTTTGGGATTACAGGCCATATGGAGTATTATGCCCATGCTGACTTGAC
AGAAATTGTCGAGCGGACGGCAGATATTTTTGAGATGGAAATCACTCATGAGGCAGCATCTGAGTTGGCCCTACGTAGTC
GTGGGACCCCTCGTATTGCCAATCGTCTCCTCAAGCGCGTGCGCGATTTTGCCCAGATAATGGGGAATGGGGTAATTGAT
GATATTATTACCGATAAGGCTTTGACTATGCTGGATGTTGACCATGAAGGTTTGGACTATGTGGATCAAAAAATCCTTCG
TACCATGATTGAGATGTACAGTGGAGGACCTGTTGGTCTAGGAACTCTTTCTGTGAATATCGCCGAAGAGCGTGAGACAG
TTGAAGACATGTATGAGCCTTACTTGATTCAAAAAGGTTTTATCATGCGGACACGGTCTGGACGGGTGGCGACTGCTAAG
GCATATGAGCACTTAGGTTATGAATACAGTGAAAAATAA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(181-254)

RuvB C-terminal winged helix domain (WHD_RuvB)

(256-325)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(20-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8CWU7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Streptococcus pneumoniae R6

100

100

1
  ruvB Streptococcus pneumoniae TIGR4

99.096

100

0.991
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.429

98.193

0.593
  ruvB Helicobacter pylori 26695

50.774

97.289

0.494
  ruvB Synechocystis sp. PCC 6803

49.84

94.277

0.47

References

[1] Joel A Lanie et al. (2007) Genome sequence of Avery's virulent serotype 2 strain D39 of Streptococcus pneumoniae and comparison with that of unencapsulated laboratory strain R6. Journal of Bacteriology 189(1):38-51. [PMID: 17041037]