Detailed information NTG1171115

Bioinformatically predicted

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: ACOQN4_RS08295
• Genome accession: NZ_OZ244735
• Coordinates: 1617149..1617547 (+)
• Length: 132 a.a.
• NCBI ID: WP_153714431.1
• Uniprot ID: A0A5Q2Q9J1
• Organism: MAG: Litorivicinus lipolyticus isolate cfb32c0c-0c5a-4fc9-8b22-2b72eeb7acf8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1612149..1622547

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACOQN4_RS08270	-	1612276..1612485 (-)	210	WP_420550363.1	DNA gyrase inhibitor YacG	-
ACOQN4_RS08275	coaE	1612482..1613081 (-)	600	WP_153714435.1	dephospho-CoA kinase	-
ACOQN4_RS08280	pilD	1613078..1613965 (-)	888	WP_420550364.1	prepilin peptidase	Machinery gene
ACOQN4_RS08285	pilC	1613990..1615234 (-)	1245	WP_153714433.1	type II secretion system F family protein	Machinery gene
ACOQN4_RS08290	pilB	1615247..1616944 (-)	1698	WP_420550365.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACOQN4_RS08295	pilA2	1617149..1617547 (+)	399	WP_153714431.1	pilin	Machinery gene
ACOQN4_RS08300	pilA	1617767..1618231 (+)	465	WP_153714430.1	pilin	Machinery gene
ACOQN4_RS08305	-	1618285..1619232 (-)	948	WP_153714429.1	diguanylate cyclase domain-containing protein	-
ACOQN4_RS08310	-	1619317..1620093 (-)	777	WP_153714428.1	YdcF family protein	-
ACOQN4_RS08315	-	1620127..1621389 (+)	1263	WP_420550366.1	hypothetical protein	-
ACOQN4_RS08320	-	1621376..1622344 (-)	969	WP_153714426.1	asparaginase	-

Sequence

Protein

• Length: 132 a.a.
• Molecular weight: 13199.13 Da
• Isoelectric Point: 6.1229

>NTDB_id=1171115 ACOQN4_RS08295 WP_153714431.1 1617149..1617547(+) (pilA2) [MAG: Litorivicinus lipolyticus isolate cfb32c0c-0c5a-4fc9-8b22-2b72eeb7acf8]
MKNQKGFTLIELMIVVAIVGILAAVAIPAYSDYTTRAKITEGLSLMSGAKTAVTENLLSGNGASSGVNDLGVTGANVASV
TTASATGAITVTYNANVAGGGNIVFTPTFANGVVSWVCTSAINDALLPATCR

Nucleotide

• Length: 399 bp

>NTDB_id=1171115 ACOQN4_RS08295 WP_153714431.1 1617149..1617547(+) (pilA2) [MAG: Litorivicinus lipolyticus isolate cfb32c0c-0c5a-4fc9-8b22-2b72eeb7acf8]
ATGAAGAATCAAAAAGGCTTTACGCTAATCGAACTGATGATCGTGGTTGCGATCGTTGGCATCTTGGCCGCTGTCGCGAT
CCCTGCGTACTCGGACTACACCACGCGCGCCAAAATTACCGAAGGCTTAAGCCTGATGTCAGGTGCCAAAACGGCGGTGA
CTGAAAACCTATTAAGCGGCAACGGTGCAAGCTCCGGTGTTAACGATCTGGGCGTGACCGGTGCCAACGTCGCCAGCGTC
ACCACTGCGTCGGCGACCGGCGCGATCACTGTAACCTACAACGCCAACGTCGCCGGCGGCGGTAACATCGTATTCACGCC
GACCTTTGCCAACGGCGTGGTCAGCTGGGTGTGTACTTCTGCTATCAACGATGCTTTGTTGCCGGCGACCTGCCGCTAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (35-132)

• Prokaryotic N-terminal methylation motif (N_methyl) (1-26)

3D structure

• AlphaFold DB: A0A5Q2Q9J1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila str. Paris	55.474	100	0.576
pilA2	Legionella pneumophila strain ERS1305867	54.015	100	0.561
comP	Acinetobacter baylyi ADP1	49.315	100	0.545
pilA	Ralstonia pseudosolanacearum GMI1000	50	100	0.508
pilE	Neisseria gonorrhoeae MS11	40.764	100	0.485
pilE	Neisseria gonorrhoeae strain FA1090	38.65	100	0.477
pilA/pilA1	Eikenella corrodens VA1	39.333	100	0.447
pilA	Pseudomonas aeruginosa PAK	38.667	100	0.439
pilA	Haemophilus influenzae Rd KW20	41.045	100	0.417
pilA	Vibrio parahaemolyticus RIMD 2210633	42.52	96.212	0.409
pilA	Haemophilus influenzae 86-028NP	39.552	100	0.402
pilA	Acinetobacter baumannii strain A118	37.41	100	0.394
pilA	Glaesserella parasuis strain SC1401	37.778	100	0.386
pilA/pilAI	Pseudomonas stutzeri DSM 10701	37.5	100	0.386
pilA/pilAII	Pseudomonas stutzeri DSM 10701	37.313	100	0.379
pilA	Vibrio cholerae C6706	33.566	100	0.364
pilA	Vibrio cholerae strain A1552	33.566	100	0.364
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	33.566	100	0.364