Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACOQN4_RS08290 Genome accession   NZ_OZ244735
Coordinates   1615247..1616944 (-) Length   565 a.a.
NCBI ID   WP_420550365.1    Uniprot ID   -
Organism   MAG: Litorivicinus lipolyticus isolate cfb32c0c-0c5a-4fc9-8b22-2b72eeb7acf8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1610247..1621944
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOQN4_RS08260 argJ 1610737..1611909 (+) 1173 WP_420550362.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  ACOQN4_RS08265 mutT 1611890..1612312 (+) 423 WP_153714437.1 8-oxo-dGTP diphosphatase MutT -
  ACOQN4_RS08270 - 1612276..1612485 (-) 210 WP_420550363.1 DNA gyrase inhibitor YacG -
  ACOQN4_RS08275 coaE 1612482..1613081 (-) 600 WP_153714435.1 dephospho-CoA kinase -
  ACOQN4_RS08280 pilD 1613078..1613965 (-) 888 WP_420550364.1 prepilin peptidase Machinery gene
  ACOQN4_RS08285 pilC 1613990..1615234 (-) 1245 WP_153714433.1 type II secretion system F family protein Machinery gene
  ACOQN4_RS08290 pilB 1615247..1616944 (-) 1698 WP_420550365.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACOQN4_RS08295 pilA2 1617149..1617547 (+) 399 WP_153714431.1 pilin Machinery gene
  ACOQN4_RS08300 pilA 1617767..1618231 (+) 465 WP_153714430.1 pilin Machinery gene
  ACOQN4_RS08305 - 1618285..1619232 (-) 948 WP_153714429.1 diguanylate cyclase domain-containing protein -
  ACOQN4_RS08310 - 1619317..1620093 (-) 777 WP_153714428.1 YdcF family protein -
  ACOQN4_RS08315 - 1620127..1621389 (+) 1263 WP_420550366.1 hypothetical protein -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 61567.38 Da        Isoelectric Point: 4.9780

>NTDB_id=1171114 ACOQN4_RS08290 WP_420550365.1 1615247..1616944(-) (pilB) [MAG: Litorivicinus lipolyticus isolate cfb32c0c-0c5a-4fc9-8b22-2b72eeb7acf8]
MGQVVLQGLARQLVSNAQISEEVAMQATADAHKAGEPFAHHVVAQSLVKSADLALTASQLYGTPLLDLNAFDLTRIPSDK
LDRKIVIEARALPLFIRGKRLYVGLSDPTDLRALDAMQFASGLTTEAIVVEADKLAAAIENYGESSGSLGDLNDDGLDNI
DLEDDSDTQTEDDPSDPSNDQPIVRFVNKMLLDAIRLGASDIHFEPYEKAYRIRYRVDGVLSEVTKPPVNMATRLAARIK
VMSKMDISERRVPQDGRIKMKLSANKAIDFRVNTLPTLWGEKIVLRILDPSSAQLGIDALGYEPDQKDMYMKALAQPQGM
ILVTGPTGSGKTVSLYTGLNILNTPEVNISTAEDPVEINLEGINQTQVNTKVGLTFAEALRAFLRQDPDVVMVGEIRDLE
TAEIAIKASQTGHLVLSTLHTNSAPETLTRLRNMGVPAFNIATSVSLIIAQRLARRLCSHCKSAVELPPEALESEGLAPA
DIATHTLYEPVGCEHCMNGYKGRVGVYEVVRVTPAISEIIMREGNALELAKQCHLEGFKSLRQSGLQKVLQGVTSLQEIN
RVTKD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=1171114 ACOQN4_RS08290 WP_420550365.1 1615247..1616944(-) (pilB) [MAG: Litorivicinus lipolyticus isolate cfb32c0c-0c5a-4fc9-8b22-2b72eeb7acf8]
GTGGGACAGGTTGTACTCCAAGGGTTAGCGCGCCAACTGGTGTCGAACGCTCAAATCAGCGAAGAAGTCGCAATGCAGGC
GACCGCGGACGCACACAAGGCCGGCGAGCCCTTTGCGCACCATGTGGTGGCGCAATCGCTGGTCAAATCGGCCGATCTCG
CGCTGACCGCTTCGCAGCTGTACGGCACCCCGCTGCTGGATCTGAACGCCTTTGATTTAACCCGCATTCCGAGCGACAAA
CTGGATCGGAAAATAGTTATCGAGGCCCGCGCCCTGCCACTGTTCATCCGCGGCAAGCGGCTGTACGTCGGCCTCAGTGA
CCCGACCGACTTGCGCGCCTTGGATGCGATGCAATTTGCCTCCGGATTGACCACCGAAGCGATTGTGGTCGAGGCCGACA
AATTGGCTGCGGCTATCGAGAACTACGGCGAATCCAGCGGTAGCCTGGGAGATCTGAATGACGACGGTTTGGACAATATT
GACCTGGAAGACGACAGCGACACCCAAACCGAGGACGATCCGAGCGACCCCAGCAACGACCAGCCCATTGTCCGCTTCGT
CAATAAGATGTTGCTCGACGCGATTCGTCTGGGTGCGTCGGACATCCACTTCGAACCCTACGAAAAAGCCTACCGCATCC
GTTACCGGGTCGACGGGGTGCTGAGTGAAGTGACCAAACCGCCGGTCAACATGGCAACCCGTTTAGCAGCGCGCATTAAA
GTGATGTCGAAAATGGACATCTCGGAGCGCCGCGTGCCCCAGGACGGGCGCATTAAGATGAAACTGTCGGCCAACAAAGC
GATCGATTTTCGCGTCAACACCCTGCCAACTTTGTGGGGTGAGAAAATCGTGCTGCGTATTTTGGACCCCAGCAGCGCCC
AACTCGGTATCGATGCGCTGGGTTACGAGCCCGACCAAAAAGACATGTACATGAAAGCGCTGGCCCAACCCCAGGGCATG
ATTTTGGTGACCGGCCCAACCGGTTCCGGCAAAACCGTGTCGCTGTACACCGGCCTGAATATTTTAAACACCCCGGAAGT
GAACATTTCGACCGCCGAAGACCCGGTCGAAATCAACTTGGAAGGTATCAACCAAACCCAGGTCAACACCAAGGTCGGGC
TGACCTTTGCCGAAGCCCTGCGGGCGTTTTTGCGCCAAGACCCGGACGTGGTCATGGTCGGCGAGATCCGTGATTTGGAA
ACCGCTGAAATTGCGATCAAGGCCTCACAAACCGGTCACCTGGTGCTGTCGACGCTGCACACCAATTCAGCCCCGGAGAC
CCTGACTCGCTTGCGCAATATGGGGGTGCCGGCTTTCAACATCGCCACCAGCGTTTCGCTAATTATTGCCCAGCGCTTGG
CGCGGCGATTATGCAGTCACTGTAAATCCGCGGTCGAGCTGCCTCCCGAGGCGCTCGAAAGCGAGGGCCTCGCGCCCGCG
GACATCGCCACGCACACGCTTTACGAACCGGTCGGGTGCGAGCACTGCATGAACGGCTACAAGGGCCGCGTCGGTGTCTA
CGAAGTGGTCCGGGTCACTCCGGCGATATCCGAAATCATCATGCGCGAGGGCAACGCGTTGGAACTGGCCAAACAGTGCC
ACCTTGAAGGGTTTAAAAGCCTTCGCCAGTCAGGCCTGCAAAAAGTCCTGCAGGGCGTGACCAGCCTTCAAGAAATTAAC
CGCGTCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.574

99.823

0.595
  pilB Acinetobacter baylyi ADP1

59.043

99.823

0.589
  pilB Legionella pneumophila strain ERS1305867

53.805

100

0.538
  pilB Vibrio cholerae strain A1552

54.286

92.92

0.504
  pilB Vibrio campbellii strain DS40M4

52.552

93.628

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

52.751

93.274

0.492
  pilF Neisseria gonorrhoeae MS11

48.754

99.469

0.485
  pilB/pilB1 Synechocystis sp. PCC 6803

41.8

88.496

0.37

Multiple sequence alignment