Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACOQN4_RS08300 Genome accession   NZ_OZ244735
Coordinates   1617767..1618231 (+) Length   154 a.a.
NCBI ID   WP_153714430.1    Uniprot ID   A0A5Q2QG11
Organism   MAG: Litorivicinus lipolyticus isolate cfb32c0c-0c5a-4fc9-8b22-2b72eeb7acf8     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1612767..1623231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOQN4_RS08280 pilD 1613078..1613965 (-) 888 WP_420550364.1 prepilin peptidase Machinery gene
  ACOQN4_RS08285 pilC 1613990..1615234 (-) 1245 WP_153714433.1 type II secretion system F family protein Machinery gene
  ACOQN4_RS08290 pilB 1615247..1616944 (-) 1698 WP_420550365.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACOQN4_RS08295 pilA2 1617149..1617547 (+) 399 WP_153714431.1 pilin Machinery gene
  ACOQN4_RS08300 pilA 1617767..1618231 (+) 465 WP_153714430.1 pilin Machinery gene
  ACOQN4_RS08305 - 1618285..1619232 (-) 948 WP_153714429.1 diguanylate cyclase domain-containing protein -
  ACOQN4_RS08310 - 1619317..1620093 (-) 777 WP_153714428.1 YdcF family protein -
  ACOQN4_RS08315 - 1620127..1621389 (+) 1263 WP_420550366.1 hypothetical protein -
  ACOQN4_RS08320 - 1621376..1622344 (-) 969 WP_153714426.1 asparaginase -
  ACOQN4_RS08325 - 1622341..1623018 (-) 678 WP_153714425.1 type 1 glutamine amidotransferase -
  ACOQN4_RS08330 - 1623015..1623203 (-) 189 WP_153714424.1 hypothetical protein -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15472.37 Da        Isoelectric Point: 5.7273

>NTDB_id=1171116 ACOQN4_RS08300 WP_153714430.1 1617767..1618231(+) (pilA) [MAG: Litorivicinus lipolyticus isolate cfb32c0c-0c5a-4fc9-8b22-2b72eeb7acf8]
MRTRTQTRAQQGFTLIELMIVVAIIGILAAVAIPAYSDYTTRAKVTEGVGIAGTIKAVVSENIIAGASSNLDAGWNSGSF
SATDNVSAVAVDGATGGITVTYTAAAGGGTMIFAPFQADGSAALSFASGAHSGNFAWDCTGGTLSANFRPANCR

Nucleotide


Download         Length: 465 bp        

>NTDB_id=1171116 ACOQN4_RS08300 WP_153714430.1 1617767..1618231(+) (pilA) [MAG: Litorivicinus lipolyticus isolate cfb32c0c-0c5a-4fc9-8b22-2b72eeb7acf8]
ATGCGTACACGCACTCAAACACGTGCCCAGCAGGGCTTTACCCTTATCGAACTGATGATCGTGGTGGCGATTATTGGCAT
ATTAGCGGCGGTGGCGATCCCTGCGTATTCCGATTACACGACGCGGGCCAAGGTCACAGAGGGCGTCGGCATCGCCGGCA
CCATTAAAGCGGTGGTCTCGGAAAACATTATTGCCGGCGCTTCATCCAACTTGGATGCGGGCTGGAACTCAGGGTCGTTT
TCAGCCACGGACAATGTGTCGGCGGTGGCCGTCGATGGCGCGACCGGCGGTATTACCGTGACCTACACGGCGGCGGCGGG
CGGCGGCACCATGATTTTTGCACCGTTCCAGGCTGACGGTTCCGCGGCGCTGTCGTTTGCATCCGGTGCCCACAGCGGTA
ACTTTGCCTGGGACTGCACCGGCGGCACTTTGAGCGCCAACTTCCGACCGGCGAATTGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-154)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q2QG11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

49.091

100

0.526
  pilA2 Legionella pneumophila strain ERS1305867

55.102

95.455

0.526
  pilA2 Legionella pneumophila str. Paris

54.422

95.455

0.519
  comP Acinetobacter baylyi ADP1

44.295

96.753

0.429

Multiple sequence alignment