Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00720  |
| T6SS type | Type i5 |
| Strain | Vibrio parahaemolyticus BB22OP |
| Replicon (RefSeq) | chromosome II (NC_019971) |
| Location | 1048650..1070709 |
| Function | anti-bacterial |
| Comment | V. parahaemolyticus T6SS2 was inactive under warm marine-like conditions in the studied RIMD 2210633 isolate, and a strong expression and secretion of the hallmark T6SS2 component Hcp2 in BB22OP under these conditions was found. Inactivation of BB22OP T6SS2 by deletion of hcp2 resulted in a significantly reduced ability to kill E. coli prey. |
| References | [1] Fridman CM, Keppel K, Gerlic M, Bosis E, Salomon D. et al (2020) A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors.. Nat Commun. 11(1):1085. [PudMed:32109231] [2] Gode-Potratz CJ et al (2011) Quorum sensing and silencing in Vibrio parahaemolyticus. J Bacteriol. 193(16):4224-37. [PudMed:21705592] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | FHA | TagJ | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| VPBB_RS19900 | 1046273..1047406 | - | 1134 | WP_015313299.1 | exonuclease SbcCD subunit D | ||
| VPBB_RS19905 | 1047503..1048345 | - | 843 | WP_015313300.1 | hypothetical protein | ||
| VPBB_RS19910 | 1048346..1048636 | - | 291 | WP_005499010.1 | PAAR domain-containing protein | ||
| T6CP007575 | TssI | VPBB_RS19915 (tssI) | 1048650..1050485 | - | 1836 | WP_015313301.1 | type VI secretion system tip protein VgrG |
| T6CP007576 | TssD | VPBB_RS19920 | 1050543..1051022 | - | 480 | WP_005375967.1 | type VI secretion system tube protein Hcp |
| T6CP007577 | TssH | VPBB_RS19925 (tssH) | 1051228..1053795 | - | 2568 | WP_015313302.1 | type VI secretion system ATPase TssH |
| T6CP007578 | TssG | VPBB_RS19930 (tssG) | 1053806..1054771 | - | 966 | WP_015313303.1 | type VI secretion system baseplate subunit TssG |
| T6CP007579 | TssF | VPBB_RS19935 (tssF) | 1054768..1056591 | - | 1824 | WP_005463819.1 | type VI secretion system baseplate subunit TssF |
| T6CP007580 | TssE | VPBB_RS19940 (tssE) | 1056588..1057052 | - | 465 | WP_015313304.1 | type VI secretion system baseplate subunit TssE |
| T6CP012862 | TagJ | VPBB_RS19945 | 1057061..1057858 | - | 798 | WP_015313305.1 | protein of avirulence locus ImpE |
| T6CP007581 | TssC | VPBB_RS19950 (tssC) | 1057869..1059380 | - | 1512 | WP_015313306.1 | type VI secretion system contractile sheath large subunit |
| T6CP007582 | TssC | VPBB_RS19955 (tssC) | 1059436..1060929 | - | 1494 | WP_005463811.1 | type VI secretion system contractile sheath large subunit |
| T6CP007583 | TssB | VPBB_RS19960 (tssB) | 1060929..1061459 | - | 531 | WP_005463809.1 | type VI secretion system contractile sheath small subunit |
| T6CP009235 | TssA | VPBB_RS19965 (tssA) | 1061475..1062596 | - | 1122 | WP_015313308.1 | type VI secretion system protein TssA |
| VPBB_RS19970 | 1062593..1063381 | - | 789 | WP_015313309.1 | protein phosphatase 2C domain-containing protein | ||
| VPBB_RS19975 (tagF) | 1063392..1064087 | - | 696 | WP_015313310.1 | type VI secretion system-associated protein TagF | ||
| T6CP007584 | TssM | VPBB_RS19980 (tssM) | 1064069..1067587 | - | 3519 | WP_029825813.1 | type VI secretion system membrane subunit TssM |
| T6CP007585 | TssL | VPBB_RS19985 (tssL) | 1067603..1068895 | - | 1293 | WP_015313312.1 | type VI secretion system protein TssL, long form |
| T6CP007586 | TssK | VPBB_RS19990 (tssK) | 1068907..1070232 | - | 1326 | WP_072828580.1 | type VI secretion system baseplate subunit TssK |
| T6CP007587 | TssJ | VPBB_RS19995 (tssJ) | 1070254..1070709 | - | 456 | WP_005463795.1 | type VI secretion system lipoprotein TssJ |
| T6CP012219 | FHA | VPBB_RS20000 (tagH) | 1070719..1071915 | - | 1197 | WP_015313314.1 | type VI secretion system-associated FHA domain protein TagH |
| VPBB_RS20010 | 1072238..1074385 | + | 2148 | WP_015313315.1 | serine/threonine protein kinase |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF01831 |
VPBB_RS19920 (Hcp2) | - | - | Vibrio parahaemolyticus BB22OP | chromosome (NC_019971) | 1050543..1051022 (-) | 491518336 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|