SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP007587
Component protein type	TssJ
T6SS ID (Type)	T6SS00720 (Type i5)
Strain	Vibrio parahaemolyticus BB22OP
Replicon	chromosome II
Sequence	Protein sequence (152 a.a.); Nucleotide sequence (456 bp)
Reference	[1] Gode-Potratz CJ, McCarter LL. Quorum sensing and silencing in Vibrio parahaemolyticus. J Bacteriol. 2011 Aug;193(16):4224-37. doi: 10.1128/JB.00432-11. Epub 2011 Jun 24. PMID: 21705592 [2] Fridman CM, Keppel K, Gerlic M, et al. A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors. Nat Commun. 2020 Feb 27;11(1):1085. doi: 10.1038/s41467-020-14951-4. PMID: 32109231

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VPBB_RS19995
Coordinate (Strand)	1070254..1070709 (-)
NCBI ID	WP_005463795.1
RefSeq	NC_019971
Uniprot ID	Q87HC3_VIBPA, A0A2R9VI00_VIBPH
KEGG ID	vpa:VPA1042
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	5.7e-39	30..145

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-6	VKQIYK
Transmembrane helix	7-26	SFLLFIFLVLMGCSAANLVV
Outside	27-151	DPYADLEITANHNINPDSNGRPSPVVVYVFELTSNTLFESQDFFSIYEEHEKVLGPDLVNKYEISLTPGQKEIYQASMSPKTEYLGIVAAFRDIENSNWRQVIKVDKTGYNTYQISLEDLSLVVQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9804	pos: 18-19. LMG-CS.	VKQIYKSFLLFIFLVLMG

Protein sequence: 152 a.a.

						>T6CP007587 NC_019971:c1070709-1070254 [Vibrio parahaemolyticus BB22OP] [TssJ]
VKQIYKSFLLFIFLVLMGCSAANLVVDPYADLEITANHNINPDSNGRPSPVVVYVFELTSNTLFESQDFFSIYEEHEKVL
GPDLVNKYEISLTPGQKEIYQASMSPKTEYLGIVAAFRDIENSNWRQVIKVDKTGYNTYQISLEDLSLVVQ*					

Nucleotide sequence: 456 bp

						>T6CP007587 NC_019971:c1070709-1070254 [Vibrio parahaemolyticus BB22OP] [TssJ]
GTGAAACAAATTTATAAATCATTTTTGCTGTTTATATTTTTAGTATTAATGGGATGCTCCGCCGCTAATTTAGTGGTCGA
CCCATATGCGGATTTAGAAATAACGGCAAATCATAATATCAACCCTGACTCGAATGGAAGACCATCACCAGTTGTTGTAT
ATGTATTTGAACTTACATCAAATACACTGTTTGAGAGCCAAGATTTTTTCTCTATTTATGAGGAGCATGAAAAAGTACTC
GGTCCAGACTTGGTGAATAAATATGAAATATCTTTGACACCAGGCCAAAAAGAAATCTACCAAGCTAGTATGTCTCCTAA
AACAGAATATTTGGGTATCGTAGCCGCGTTTCGCGATATTGAGAATTCAAATTGGCGACAAGTCATTAAAGTAGATAAGA
CGGGTTACAACACTTATCAAATCTCGTTAGAAGATCTGTCTTTGGTCGTACAATAA					