SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP007578
Component protein type	TssG
T6SS ID (Type)	T6SS00720 (Type i5)
Strain	Vibrio parahaemolyticus BB22OP
Replicon	chromosome II
Sequence	Protein sequence (322 a.a.); Nucleotide sequence (966 bp)
Reference	[1] Gode-Potratz CJ, McCarter LL. Quorum sensing and silencing in Vibrio parahaemolyticus. J Bacteriol. 2011 Aug;193(16):4224-37. doi: 10.1128/JB.00432-11. Epub 2011 Jun 24. PMID: 21705592 [2] Fridman CM, Keppel K, Gerlic M, et al. A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors. Nat Commun. 2020 Feb 27;11(1):1085. doi: 10.1038/s41467-020-14951-4. PMID: 32109231

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VPBB_RS19930
Coordinate (Strand)	1053806..1054771 (-)
NCBI ID	WP_015313303.1
RefSeq	NC_019971
Uniprot ID	UPI0002A5811C
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	1.7e-69	11..306

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-321	MSTEDFYRELKQRSLFEAIHLIEQELLKVESQIGTDALPKNEKLNLKVNASLGFENAQLVSTKPLGKDKLALETNLIGLTGEQGVLPQHYSELALHRLKEGDHAMVDFYDIFNHRLLSLYYRSWQLSQLTIQARAHAKNQRSPLTDCMSSLTGGGNDLALHYGGMYASPTRSKGALKSILECLSGCQIRIHEFQGQWMRLSKSEQTRLVSKSMPEGQFAQLGAGASLGKKAWNINASTTIEFLPKESKQVTNLLPKTNTLKTIKQVAGDFIGKHKHVKWQLTTKHSLLPQVQISKQQGQLGVGSVLKKHERTEDRNITITV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9919	-	-

Protein sequence: 322 a.a.

						>T6CP007578 NC_019971:c1054771-1053806 [Vibrio parahaemolyticus BB22OP] [TssG]
MSTEDFYRELKQRSLFEAIHLIEQELLKVESQIGTDALPKNEKLNLKVNASLGFENAQLVSTKPLGKDKLALETNLIGLT
GEQGVLPQHYSELALHRLKEGDHAMVDFYDIFNHRLLSLYYRSWQLSQLTIQARAHAKNQRSPLTDCMSSLTGGGNDLAL
HYGGMYASPTRSKGALKSILECLSGCQIRIHEFQGQWMRLSKSEQTRLVSKSMPEGQFAQLGAGASLGKKAWNINASTTI
EFLPKESKQVTNLLPKTNTLKTIKQVAGDFIGKHKHVKWQLTTKHSLLPQVQISKQQGQLGVGSVLKKHERTEDRNITIT
V*					

Nucleotide sequence: 966 bp

						>T6CP007578 NC_019971:c1054771-1053806 [Vibrio parahaemolyticus BB22OP] [TssG]
ATGAGCACAGAGGACTTCTACCGCGAGCTAAAGCAACGTTCTTTGTTTGAAGCTATTCATCTTATTGAGCAAGAGCTTTT
GAAAGTTGAATCGCAAATTGGAACAGATGCATTACCCAAAAACGAGAAACTTAACTTAAAGGTCAATGCGTCTTTAGGGT
TTGAGAATGCGCAACTCGTTTCTACTAAACCATTAGGTAAAGATAAATTAGCGCTTGAGACAAATTTGATTGGCTTGACG
GGGGAGCAGGGTGTACTGCCTCAGCATTACAGTGAGCTTGCATTACATCGACTGAAAGAAGGCGATCATGCGATGGTGGA
CTTCTATGACATTTTTAACCATCGGCTTTTATCCCTCTATTATCGAAGTTGGCAGTTGAGTCAGTTAACCATACAAGCGC
GTGCGCATGCAAAAAATCAGCGCTCCCCATTAACTGATTGCATGTCGTCATTGACTGGCGGTGGGAATGATCTAGCACTG
CATTATGGCGGCATGTATGCATCTCCAACACGAAGTAAAGGTGCATTGAAGAGCATTTTGGAATGTTTGTCTGGTTGCCA
AATTCGTATTCACGAGTTTCAAGGTCAATGGATGCGACTGTCTAAATCTGAACAAACTCGTTTGGTATCGAAAAGTATGC
CCGAAGGCCAGTTTGCACAACTCGGTGCTGGTGCGTCACTAGGGAAAAAAGCCTGGAATATCAATGCAAGTACAACAATT
GAGTTTCTCCCAAAGGAAAGTAAGCAAGTCACAAATCTATTACCCAAGACCAATACATTGAAAACGATAAAACAGGTAGC
AGGTGACTTCATTGGCAAACACAAGCACGTTAAATGGCAACTCACGACTAAACATAGTCTATTGCCTCAAGTTCAAATAT
CTAAGCAACAAGGTCAGTTAGGTGTCGGCTCTGTACTGAAAAAACATGAACGTACGGAAGATCGCAACATAACAATTACG
GTTTAA					