SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP009235
Component protein type	TssA
T6SS ID (Type)	T6SS00720 (Type i5)
Strain	Vibrio parahaemolyticus BB22OP
Replicon	chromosome II
Sequence	Protein sequence (374 a.a.); Nucleotide sequence (1122 bp)
Reference	[1] Gode-Potratz CJ, McCarter LL. Quorum sensing and silencing in Vibrio parahaemolyticus. J Bacteriol. 2011 Aug;193(16):4224-37. doi: 10.1128/JB.00432-11. Epub 2011 Jun 24. PMID: 21705592 [2] Fridman CM, Keppel K, Gerlic M, et al. A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors. Nat Commun. 2020 Feb 27;11(1):1085. doi: 10.1038/s41467-020-14951-4. PMID: 32109231

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VPBB_RS19965
Coordinate (Strand)	1061475..1062596 (-)
NCBI ID	WP_015313308.1
RefSeq	NC_019971
Uniprot ID	UPI0002A57A56
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	2.2e-31	13..142

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-373	MSNTMFDWYEEVLAPVSEDNPTGIDPREDVSPQSAYYRLKDQRMVARNAERNAIIEEESIHTHNNLWRVFVEEVPEVLATQSKDFEFVAWLIEALTRLYGFRGMGVGYKIASAFVESYWDELHPMPDEDGIETRISAITGLNGIDSEGTLIFPLASIPLTDMGVEQAYAYWEYQQAIELERLDEDKRRARVDQGAIELSRITDTVKSTSDEFYQSLIADLEFATGAFNEFSQRLDEAVGDVTPSSYISKRIESIHAALKHLLGNRFKDKAEVSISDENGDEELEEASQQAVTHNSSALLVTNMQSREQAIQQLQHVADFFRESEPHSPVSYSIEQIIRWCGMPLPELLAELISDGDAKRSYFRLVGIAEQNEN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9892	-	-

Protein sequence: 374 a.a.

						>T6CP009235 NC_019971:c1062596-1061475 [Vibrio parahaemolyticus BB22OP] [TssA]
MSNTMFDWYEEVLAPVSEDNPTGIDPREDVSPQSAYYRLKDQRMVARNAERNAIIEEESIHTHNNLWRVFVEEVPEVLAT
QSKDFEFVAWLIEALTRLYGFRGMGVGYKIASAFVESYWDELHPMPDEDGIETRISAITGLNGIDSEGTLIFPLASIPLT
DMGVEQAYAYWEYQQAIELERLDEDKRRARVDQGAIELSRITDTVKSTSDEFYQSLIADLEFATGAFNEFSQRLDEAVGD
VTPSSYISKRIESIHAALKHLLGNRFKDKAEVSISDENGDEELEEASQQAVTHNSSALLVTNMQSREQAIQQLQHVADFF
RESEPHSPVSYSIEQIIRWCGMPLPELLAELISDGDAKRSYFRLVGIAEQNEN*					

Nucleotide sequence: 1122 bp

						>T6CP009235 NC_019971:c1062596-1061475 [Vibrio parahaemolyticus BB22OP] [TssA]
ATGAGTAACACGATGTTTGATTGGTATGAGGAAGTCCTTGCACCAGTTTCGGAAGACAATCCGACTGGCATTGATCCAAG
AGAGGATGTCTCTCCTCAGTCTGCTTATTATCGACTTAAAGATCAGCGAATGGTAGCAAGAAACGCAGAGCGAAACGCCA
TCATCGAAGAAGAAAGTATTCACACACATAACAACTTATGGCGCGTTTTTGTTGAAGAAGTACCAGAAGTTCTCGCTACA
CAATCAAAAGATTTTGAGTTTGTTGCCTGGCTAATAGAAGCATTAACTCGGCTTTATGGTTTTAGAGGCATGGGCGTAGG
CTACAAAATTGCTAGCGCGTTTGTTGAGTCATATTGGGATGAGTTGCACCCAATGCCTGATGAAGACGGCATAGAAACGC
GGATTTCTGCCATCACCGGACTTAATGGTATTGATAGTGAGGGAACGCTGATCTTTCCTCTTGCCTCGATTCCGTTGACG
GATATGGGGGTAGAGCAAGCTTATGCATATTGGGAATACCAACAAGCCATTGAACTCGAGCGCTTGGACGAAGACAAACG
AAGAGCGCGAGTAGATCAAGGCGCAATTGAATTGTCGCGTATCACTGACACTGTGAAATCAACGTCAGATGAGTTTTACC
AGTCTCTGATTGCAGATCTCGAGTTCGCTACAGGTGCTTTTAATGAGTTCAGTCAGCGACTTGATGAGGCTGTAGGGGAT
GTAACGCCTAGCTCATATATTTCAAAAAGAATTGAATCAATACACGCGGCACTTAAGCACTTGTTGGGTAATCGTTTTAA
GGATAAGGCTGAGGTCTCCATTTCTGACGAAAATGGTGATGAAGAGTTAGAAGAAGCAAGTCAACAAGCAGTTACTCACA
ACTCATCTGCGTTATTAGTTACAAACATGCAATCTCGAGAACAAGCTATTCAACAGTTGCAACATGTGGCGGACTTTTTT
AGAGAAAGCGAGCCTCACTCACCAGTTTCTTACTCCATCGAACAAATTATTCGTTGGTGTGGAATGCCTCTGCCTGAATT
ACTGGCGGAACTAATCTCCGACGGAGACGCAAAACGAAGCTACTTCCGTTTGGTTGGCATAGCAGAACAAAATGAAAATT
AA					