SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP007583
Component protein type	TssB
T6SS ID (Type)	T6SS00720 (Type i5)
Strain	Vibrio parahaemolyticus BB22OP
Replicon	chromosome II
Sequence	Protein sequence (177 a.a.); Nucleotide sequence (531 bp)
Reference	[1] Gode-Potratz CJ, McCarter LL. Quorum sensing and silencing in Vibrio parahaemolyticus. J Bacteriol. 2011 Aug;193(16):4224-37. doi: 10.1128/JB.00432-11. Epub 2011 Jun 24. PMID: 21705592 [2] Fridman CM, Keppel K, Gerlic M, et al. A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors. Nat Commun. 2020 Feb 27;11(1):1085. doi: 10.1038/s41467-020-14951-4. PMID: 32109231

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VPBB_RS19960
Coordinate (Strand)	1060929..1061459 (-)
NCBI ID	WP_005463809.1
RefSeq	NC_019971
Uniprot ID	S5JDR9_VIBPH, Q87HD0_VIBPA, A0A072FX85_VIBPH
KEGG ID	vpa:VPA1035, vpf:M634_23095
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	1.6e-62	9..161

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-176	MTSIHSKLSRVRKPRVHITYDVETEGTTLKKELPFVVGVMGDFAGQNTEALKPLKDRRFIQIDRDNFDDVLKKMSPSVKFKVANKLANDDSEFAVELSFKSMQDFEPAAIVNQVEPLRQLMETRNKLRDLMTKVDRSEELENILEEVLNNTDNLAKLAGELDLGKKAPSESTEGAE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9966	-	-

Protein sequence: 177 a.a.

						>T6CP007583 NC_019971:c1061459-1060929 [Vibrio parahaemolyticus BB22OP] [TssB]
MTSIHSKLSRVRKPRVHITYDVETEGTTLKKELPFVVGVMGDFAGQNTEALKPLKDRRFIQIDRDNFDDVLKKMSPSVKF
KVANKLANDDSEFAVELSFKSMQDFEPAAIVNQVEPLRQLMETRNKLRDLMTKVDRSEELENILEEVLNNTDNLAKLAGE
LDLGKKAPSESTEGAE*					

Nucleotide sequence: 531 bp

						>T6CP007583 NC_019971:c1061459-1060929 [Vibrio parahaemolyticus BB22OP] [TssB]
ATGACGAGTATCCACTCGAAACTTTCACGAGTACGGAAGCCTCGTGTTCACATTACCTACGATGTTGAAACCGAAGGAAC
AACTTTGAAAAAAGAGCTTCCTTTTGTGGTGGGTGTTATGGGCGACTTTGCAGGCCAAAATACCGAAGCATTGAAACCAT
TAAAGGATCGCCGTTTCATTCAAATCGATCGTGATAACTTCGATGATGTACTTAAAAAGATGAGTCCATCGGTTAAGTTC
AAAGTCGCAAACAAGTTAGCAAATGACGATTCTGAATTTGCGGTTGAACTGAGCTTTAAATCGATGCAAGACTTTGAACC
AGCAGCGATTGTCAATCAAGTAGAACCATTGCGTCAGCTGATGGAAACTCGTAACAAACTGCGAGACTTGATGACTAAAG
TCGACCGTTCGGAAGAGCTTGAAAACATCCTAGAAGAAGTTCTCAACAATACAGACAATTTGGCAAAACTAGCTGGTGAG
CTAGATCTTGGCAAAAAAGCGCCATCAGAAAGTACTGAGGGAGCAGAATAA					