SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP007584
Component protein type	TssM
T6SS ID (Type)	T6SS00720 (Type i5)
Strain	Vibrio parahaemolyticus BB22OP
Replicon	chromosome II
Sequence	Protein sequence (1173 a.a.); Nucleotide sequence (3519 bp)
Reference	[1] Gode-Potratz CJ, McCarter LL. Quorum sensing and silencing in Vibrio parahaemolyticus. J Bacteriol. 2011 Aug;193(16):4224-37. doi: 10.1128/JB.00432-11. Epub 2011 Jun 24. PMID: 21705592 [2] Fridman CM, Keppel K, Gerlic M, et al. A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors. Nat Commun. 2020 Feb 27;11(1):1085. doi: 10.1038/s41467-020-14951-4. PMID: 32109231

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VPBB_RS19980
Coordinate (Strand)	1064069..1067587 (-)
NCBI ID	WP_029825813.1
RefSeq	NC_019971
Uniprot ID	UPI0004A3EDFF
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related_N	PF14331.8	2e-85	193..450
IcmF-related	PF06761.14	3.7e-76	504..802
IcmF_C	PF06744.14	3.6e-18	1049..1149

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-12	MSIKEIGRVFTQ
Transmembrane helix	13-32	RWFLGLVGVAACSIFIWVVG
Outside	33-46	PLITVAGYEPLKSD
Transmembrane helix	47-64	FQRLVTILVIVFAWALIN
Inside	65-445	LTKQHKQKVREDESIQTLLEVDSQSDKEAASEIDVMRDRIEQAIKVVTKTHKGKRSLYDLPWYVLIGPPGTGKTTVLKQSGLEFPLAESLGADSIAGVGGTRHCDWWFANKAVLIDTAGRYTTQDSQEKVDSKAWHGFLGLLKKYRTQRPINGAIVTVSLASVMSQTRTERSLHARSIKSRLQELKNQLGMQFPIYVLLTKMDLVAGFNEFFADLSKEEREELFGFMFSREVDDERGVISLFNKEFHGMLERLDARMLRILETEDDLEKRTLIFEFPKQLRVLQANLDEFLSEIFAQNTFEEPALIRGVFLLSSVQEGIPVDRLMSESTHGLGLGRLPLATNVNSSHSYFVKNLFERVIFKEQLLGTVNRHYQKQSGWMRT
Transmembrane helix	446-468	GIYVGCVGVLVGASALWFLSYQW
Outside	469-1172	NSKLIVDTNSQVNHIEAMIGAESLDFESDVISAVDTLDKIMMLPLGKNSKYGHSDAVKKFGLYQGDKVSQAANNAYSDALSQHFATLLSESLVSEMEANKQHREYLYETLKTYLMLFNPEKYQQEEVIAWFNFYFERQYPGELNKELRERLLVHTKNLLENDEKGFSMDATAISAAREVLTQMSLPERAYQRMKMQFAKSHVPSFRLTDVLGPKGLEQFERASGKPLSQGISGFYTYNGFHSIFQIQINRTVKGLMEENWVYGDDLKAHEIDHDSAIQGVQARYYQDYVNEWKTLIEDIQLKQAPSLALATEQSRVLSGVERPIESLLRAIQKEVGLSKVTLSENQKAATEVAGKVAKVKFSNTADKLDMYLPEENGFNVALPGKEVESHFTEILRLSEQDFDDIQQAMVNLRSYLSDLSSSGNNQKIAYKSILDGTVTHDVAASFARAKDLLPKPFNQWLGELSQESVKFAESGSKDHLNQLWVTNVVRPYQRTIAGRYPFEPNATKEVRLKDFQRFFGYGGTLDSFFQEYLEPFVDTSKSRWRLEKEIGVRPETLAVFQRAKRIRQSFFESDNSLRVEFGMKPVYLDQHITRFVLELGDQDLVYKHGPARSKELRWPSGQDQTRIVFTPPESKREIAHTYEGEWGIFKLLDQSLKARPESRNDNIVMIDLKGNKVQLELIPSSAINPFWSNEMERFRCPQTL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.6736	-	-

Protein sequence: 1173 a.a.

						>T6CP007584 NC_019971:c1067587-1064069 [Vibrio parahaemolyticus BB22OP] [TssM]
MSIKEIGRVFTQRWFLGLVGVAACSIFIWVVGPLITVAGYEPLKSDFQRLVTILVIVFAWALINLTKQHKQKVREDESIQ
TLLEVDSQSDKEAASEIDVMRDRIEQAIKVVTKTHKGKRSLYDLPWYVLIGPPGTGKTTVLKQSGLEFPLAESLGADSIA
GVGGTRHCDWWFANKAVLIDTAGRYTTQDSQEKVDSKAWHGFLGLLKKYRTQRPINGAIVTVSLASVMSQTRTERSLHAR
SIKSRLQELKNQLGMQFPIYVLLTKMDLVAGFNEFFADLSKEEREELFGFMFSREVDDERGVISLFNKEFHGMLERLDAR
MLRILETEDDLEKRTLIFEFPKQLRVLQANLDEFLSEIFAQNTFEEPALIRGVFLLSSVQEGIPVDRLMSESTHGLGLGR
LPLATNVNSSHSYFVKNLFERVIFKEQLLGTVNRHYQKQSGWMRTGIYVGCVGVLVGASALWFLSYQWNSKLIVDTNSQV
NHIEAMIGAESLDFESDVISAVDTLDKIMMLPLGKNSKYGHSDAVKKFGLYQGDKVSQAANNAYSDALSQHFATLLSESL
VSEMEANKQHREYLYETLKTYLMLFNPEKYQQEEVIAWFNFYFERQYPGELNKELRERLLVHTKNLLENDEKGFSMDATA
ISAAREVLTQMSLPERAYQRMKMQFAKSHVPSFRLTDVLGPKGLEQFERASGKPLSQGISGFYTYNGFHSIFQIQINRTV
KGLMEENWVYGDDLKAHEIDHDSAIQGVQARYYQDYVNEWKTLIEDIQLKQAPSLALATEQSRVLSGVERPIESLLRAIQ
KEVGLSKVTLSENQKAATEVAGKVAKVKFSNTADKLDMYLPEENGFNVALPGKEVESHFTEILRLSEQDFDDIQQAMVNL
RSYLSDLSSSGNNQKIAYKSILDGTVTHDVAASFARAKDLLPKPFNQWLGELSQESVKFAESGSKDHLNQLWVTNVVRPY
QRTIAGRYPFEPNATKEVRLKDFQRFFGYGGTLDSFFQEYLEPFVDTSKSRWRLEKEIGVRPETLAVFQRAKRIRQSFFE
SDNSLRVEFGMKPVYLDQHITRFVLELGDQDLVYKHGPARSKELRWPSGQDQTRIVFTPPESKREIAHTYEGEWGIFKLL
DQSLKARPESRNDNIVMIDLKGNKVQLELIPSSAINPFWSNEMERFRCPQTL*					

Nucleotide sequence: 3519 bp

						>T6CP007584 NC_019971:c1067587-1064069 [Vibrio parahaemolyticus BB22OP] [TssM]
ATGAGTATTAAAGAAATAGGCAGAGTATTTACCCAAAGATGGTTTCTTGGTTTAGTTGGTGTAGCCGCGTGCTCCATTTT
TATTTGGGTTGTGGGTCCTCTGATCACGGTTGCAGGTTACGAGCCGCTTAAAAGTGATTTCCAACGTTTAGTTACGATCT
TAGTGATTGTTTTTGCTTGGGCGCTTATCAACTTAACAAAGCAGCACAAACAGAAAGTTAGAGAAGACGAAAGTATTCAG
ACGTTGCTTGAAGTAGACAGTCAGTCAGACAAAGAAGCCGCCAGTGAAATTGACGTAATGCGCGATCGTATAGAGCAAGC
GATCAAAGTTGTGACAAAAACGCATAAAGGAAAGCGTAGTTTGTACGATTTACCTTGGTACGTTCTGATTGGTCCTCCTG
GAACAGGTAAAACCACGGTTCTAAAGCAATCTGGCTTGGAATTTCCGTTAGCTGAGAGCTTAGGGGCTGACTCTATAGCT
GGTGTAGGTGGAACACGTCACTGTGATTGGTGGTTTGCTAATAAAGCAGTACTTATTGATACAGCAGGACGTTACACCAC
GCAAGATAGTCAAGAAAAGGTTGACTCTAAAGCTTGGCACGGTTTCTTGGGGTTACTTAAGAAATATCGTACTCAAAGGC
CTATCAATGGCGCTATCGTAACAGTCAGCCTAGCAAGTGTGATGTCTCAAACTCGTACGGAGCGTAGCTTACATGCCAGA
TCAATAAAATCTCGCCTTCAGGAACTCAAAAATCAGTTGGGTATGCAATTCCCAATCTATGTTCTGCTGACGAAAATGGA
TCTGGTTGCTGGCTTTAATGAATTTTTTGCTGATTTAAGTAAAGAAGAGCGTGAGGAACTTTTTGGATTTATGTTCTCAC
GAGAAGTTGATGACGAACGTGGTGTCATTTCTCTCTTTAATAAAGAGTTTCACGGCATGTTGGAGCGGCTCGATGCGCGC
ATGTTACGTATATTAGAAACTGAAGATGACTTAGAAAAGCGCACGCTAATATTCGAATTTCCAAAACAGCTGCGAGTTTT
ACAAGCAAACTTAGATGAGTTTTTGTCTGAGATATTTGCGCAGAACACGTTTGAAGAGCCAGCCCTTATTCGCGGTGTTT
TCTTGCTTAGCTCTGTACAAGAAGGCATTCCTGTTGATCGACTGATGAGTGAATCAACTCATGGATTGGGACTAGGGAGG
TTGCCTTTAGCGACAAACGTTAACAGTTCGCATAGCTATTTTGTTAAAAATCTTTTTGAGCGAGTCATCTTTAAAGAGCA
ATTACTAGGTACCGTTAACCGCCACTATCAAAAGCAATCTGGATGGATGCGTACGGGCATATACGTGGGTTGTGTTGGTG
TTCTTGTTGGTGCTTCTGCTCTTTGGTTCCTGAGTTATCAATGGAATTCAAAGTTAATTGTCGATACCAATAGCCAAGTA
AATCATATTGAAGCGATGATAGGTGCAGAGAGCCTTGATTTTGAATCGGACGTTATCAGTGCTGTAGACACGTTAGACAA
GATCATGATGTTGCCACTAGGGAAAAATAGTAAATATGGTCATTCTGATGCAGTGAAAAAGTTTGGTCTGTATCAAGGTG
ACAAAGTTTCTCAAGCAGCAAATAACGCGTATTCAGATGCTCTTTCTCAACACTTTGCTACTCTGTTGTCAGAGAGTTTG
GTCTCGGAAATGGAAGCGAATAAGCAGCATCGTGAATACTTATACGAGACGTTAAAGACGTATTTAATGTTGTTTAATCC
GGAAAAGTATCAACAAGAAGAGGTAATTGCTTGGTTCAACTTCTACTTTGAACGTCAGTATCCAGGAGAGTTAAACAAAG
AGCTACGTGAGCGTTTGCTAGTGCATACTAAAAACTTACTAGAGAACGACGAGAAAGGCTTCTCAATGGATGCGACTGCT
ATTAGTGCAGCACGAGAAGTTCTAACGCAAATGTCACTTCCAGAGCGTGCATATCAGCGTATGAAGATGCAGTTTGCTAA
AAGCCACGTTCCGTCTTTCCGTTTAACAGACGTGTTAGGACCTAAAGGTTTAGAACAATTTGAGCGAGCAAGTGGAAAAC
CTCTATCGCAAGGTATTTCCGGTTTTTATACCTACAACGGTTTTCACAGCATTTTCCAAATTCAGATTAATCGCACCGTC
AAAGGTTTGATGGAAGAAAACTGGGTGTATGGGGATGATCTTAAAGCTCATGAAATTGATCATGATAGTGCTATTCAGGG
GGTTCAAGCCCGCTATTATCAAGATTATGTCAATGAGTGGAAAACTCTGATAGAGGATATTCAGCTTAAACAAGCTCCGA
GTTTGGCTTTAGCAACAGAGCAATCAAGAGTTTTATCCGGTGTAGAAAGACCAATTGAATCACTTCTGCGTGCAATACAA
AAGGAAGTGGGGTTATCGAAAGTAACGTTATCGGAAAATCAAAAGGCAGCAACGGAAGTCGCAGGAAAGGTTGCAAAAGT
GAAGTTTTCTAATACGGCAGACAAACTCGATATGTATTTGCCTGAAGAGAATGGATTTAATGTTGCTCTACCAGGTAAAG
AAGTTGAATCCCATTTCACCGAAATCTTACGACTTAGTGAACAAGACTTTGATGATATTCAGCAAGCGATGGTGAATTTG
CGTTCGTACTTATCTGATTTGTCTAGCTCTGGCAACAATCAAAAAATTGCTTACAAAAGCATATTGGATGGAACGGTGAC
TCATGATGTTGCTGCCTCCTTTGCTAGAGCAAAAGATCTCTTACCAAAACCGTTTAATCAATGGTTGGGAGAGTTGTCAC
AGGAATCGGTTAAGTTTGCAGAAAGCGGCTCTAAAGATCATCTAAATCAGCTTTGGGTGACTAATGTAGTGCGTCCTTAT
CAGCGGACTATAGCAGGTAGATACCCATTTGAACCTAATGCAACGAAGGAAGTTCGTCTAAAGGACTTTCAACGATTCTT
CGGGTACGGTGGTACGTTGGACTCGTTCTTCCAAGAATATCTGGAGCCGTTTGTTGATACGTCTAAGAGTCGTTGGAGAC
TAGAAAAAGAAATCGGTGTAAGGCCCGAGACGCTTGCAGTTTTTCAAAGGGCAAAACGCATTAGACAATCGTTCTTTGAA
TCAGATAACTCGCTACGAGTCGAGTTTGGTATGAAACCCGTATATCTAGATCAACACATTACTCGATTTGTGCTAGAGCT
AGGTGATCAAGACTTGGTTTATAAGCACGGTCCTGCGAGATCGAAGGAACTTCGCTGGCCATCGGGTCAAGATCAGACGC
GTATTGTGTTTACACCGCCCGAATCAAAACGAGAAATTGCTCATACCTATGAAGGGGAATGGGGCATTTTCAAACTATTG
GACCAATCACTCAAGGCTCGACCAGAGTCTCGCAACGACAACATTGTCATGATCGATTTGAAGGGGAACAAGGTTCAGCT
TGAGTTGATTCCATCTAGTGCAATCAACCCATTTTGGTCTAATGAGATGGAGAGGTTCAGATGTCCGCAAACCTTGTAA					