SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP007579
Component protein type	TssF
T6SS ID (Type)	T6SS00720 (Type i5)
Strain	Vibrio parahaemolyticus BB22OP
Replicon	chromosome II
Sequence	Protein sequence (608 a.a.); Nucleotide sequence (1824 bp)
Reference	[1] Gode-Potratz CJ, McCarter LL. Quorum sensing and silencing in Vibrio parahaemolyticus. J Bacteriol. 2011 Aug;193(16):4224-37. doi: 10.1128/JB.00432-11. Epub 2011 Jun 24. PMID: 21705592 [2] Fridman CM, Keppel K, Gerlic M, et al. A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors. Nat Commun. 2020 Feb 27;11(1):1085. doi: 10.1038/s41467-020-14951-4. PMID: 32109231

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VPBB_RS19935
Coordinate (Strand)	1054768..1056591 (-)
NCBI ID	WP_005463819.1
RefSeq	NC_019971
Uniprot ID	Q87HD5_VIBPA, A0A7Y0S4J1_VIBPH
KEGG ID	vpa:VPA1030
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	1.3e-187	1..603

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-607	MSEEFLKYYNRELAYLRHKGQEFGEQYPKIAARLRISEEQVEDPHVERLLEGCAFLTARIRQSLDNSYPQFTESLMGQLYPDFHAPIPSMSVIKLNCSESTTSSFAIPVGERVEVSAPGYQDCQFRTGYPTTMYPLDIISGSFENAPFKPTGSKWEHNAHSVLRCQLSAHDSESSINSMGIDCLRLYLNGQTQLTLKLYQMLFQSLIGLSIVLDGKEIMPLTKRHLQSVGFGDEEQVVPYSKRSFSGSRLLVEYLHFPEKFMFFDLIELGLDKLEIQGQVEICFYFDTSDDWLPKQVDEESVMVGCVPIVNLFKSTMEPKRLLPTEYEYQLSPQYQDAESNEVVSISDVTLRNWNKTYEHLPCYHSGEHTHYMSASEVYWLMRREDKNWAGGYDEPGRETYVSFVDKQYQLFSPESRDNWLMYVEAECCNRNLPQKIPFGGGLPKVQLPNVDDNFKSIRCLTSLSETLRPEMDESTRWQLTKLLTLNHFTEADGLATLKQTLNLYAFAGTAETKAVIDALVKLEFEHTTGRVSQKGKVGFAHGVKLVLTVSDQILPKEQIFLLGSVLSVYFAQYAEINVFTQLEIKLKSTSSSFHVWPALTGDKVLL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9953	-	-

Protein sequence: 608 a.a.

						>T6CP007579 NC_019971:c1056591-1054768 [Vibrio parahaemolyticus BB22OP] [TssF]
MSEEFLKYYNRELAYLRHKGQEFGEQYPKIAARLRISEEQVEDPHVERLLEGCAFLTARIRQSLDNSYPQFTESLMGQLY
PDFHAPIPSMSVIKLNCSESTTSSFAIPVGERVEVSAPGYQDCQFRTGYPTTMYPLDIISGSFENAPFKPTGSKWEHNAH
SVLRCQLSAHDSESSINSMGIDCLRLYLNGQTQLTLKLYQMLFQSLIGLSIVLDGKEIMPLTKRHLQSVGFGDEEQVVPY
SKRSFSGSRLLVEYLHFPEKFMFFDLIELGLDKLEIQGQVEICFYFDTSDDWLPKQVDEESVMVGCVPIVNLFKSTMEPK
RLLPTEYEYQLSPQYQDAESNEVVSISDVTLRNWNKTYEHLPCYHSGEHTHYMSASEVYWLMRREDKNWAGGYDEPGRET
YVSFVDKQYQLFSPESRDNWLMYVEAECCNRNLPQKIPFGGGLPKVQLPNVDDNFKSIRCLTSLSETLRPEMDESTRWQL
TKLLTLNHFTEADGLATLKQTLNLYAFAGTAETKAVIDALVKLEFEHTTGRVSQKGKVGFAHGVKLVLTVSDQILPKEQI
FLLGSVLSVYFAQYAEINVFTQLEIKLKSTSSSFHVWPALTGDKVLL*					

Nucleotide sequence: 1824 bp

						>T6CP007579 NC_019971:c1056591-1054768 [Vibrio parahaemolyticus BB22OP] [TssF]
ATGAGTGAAGAGTTTCTAAAATACTACAATCGTGAATTGGCCTATCTGCGACACAAAGGACAAGAGTTTGGCGAGCAATA
TCCTAAAATTGCGGCTAGGTTACGTATCAGCGAAGAACAAGTTGAAGACCCACATGTTGAACGTCTGTTGGAAGGTTGTG
CTTTTTTAACCGCTCGGATAAGACAAAGTCTGGATAACAGTTACCCACAGTTTACGGAATCACTGATGGGACAGTTATAT
CCGGACTTTCATGCGCCAATTCCTTCGATGTCAGTGATCAAATTGAACTGTTCAGAGTCAACCACTAGTTCATTTGCTAT
TCCTGTGGGTGAAAGAGTCGAAGTTAGCGCCCCTGGATATCAAGATTGCCAGTTTCGAACCGGTTACCCGACAACAATGT
ATCCACTCGATATTATCTCTGGTTCATTTGAGAACGCGCCTTTCAAGCCTACTGGCTCTAAGTGGGAGCACAATGCGCAC
TCTGTACTAAGGTGTCAGCTGAGTGCTCATGATTCAGAGTCCTCTATCAATTCCATGGGTATCGATTGTTTGCGTTTGTA
CTTAAATGGACAGACGCAATTAACACTCAAGCTATATCAAATGTTATTCCAAAGTTTGATTGGTCTTTCAATCGTGTTGG
ATGGTAAAGAAATCATGCCTCTAACTAAGAGGCATCTTCAATCGGTTGGTTTTGGGGACGAGGAGCAAGTCGTCCCTTAC
AGTAAGAGAAGTTTTTCGGGATCTCGTTTATTGGTCGAGTACCTTCATTTCCCAGAAAAATTCATGTTCTTTGACTTGAT
TGAGCTCGGCTTAGATAAGTTAGAGATTCAAGGGCAGGTCGAGATTTGTTTTTACTTCGATACAAGCGATGACTGGCTAC
CAAAGCAAGTAGACGAGGAAAGTGTCATGGTTGGCTGTGTTCCCATTGTAAATCTGTTCAAATCAACCATGGAACCTAAA
CGTCTCCTTCCAACAGAGTACGAGTATCAATTATCTCCTCAGTACCAAGATGCAGAGAGTAATGAAGTTGTCTCGATTTC
TGATGTGACCTTGAGAAACTGGAACAAAACTTACGAGCACTTACCCTGCTACCATTCAGGAGAGCACACGCACTACATGA
GCGCGAGTGAAGTGTATTGGTTAATGAGACGAGAGGATAAAAACTGGGCCGGTGGCTACGATGAGCCTGGTCGAGAGACT
TATGTTTCTTTTGTTGATAAGCAATACCAGTTGTTCAGCCCTGAAAGCAGAGATAATTGGTTGATGTATGTGGAAGCTGA
GTGTTGCAATCGTAATTTGCCACAAAAAATTCCATTTGGTGGCGGGCTACCAAAGGTTCAGTTACCCAATGTTGATGATA
ATTTTAAATCAATCAGGTGCTTAACGTCTCTCTCCGAAACGCTTAGGCCAGAGATGGATGAAAGCACACGATGGCAGCTT
ACCAAGCTATTAACATTGAACCATTTTACTGAAGCTGACGGCTTAGCAACGTTAAAACAAACATTGAACTTGTATGCATT
TGCAGGCACTGCCGAAACAAAAGCCGTTATAGATGCTCTAGTCAAGCTAGAGTTTGAACATACAACCGGTAGGGTTAGTC
AGAAAGGAAAAGTTGGTTTTGCACATGGAGTAAAGCTGGTTCTAACAGTGTCCGATCAGATACTGCCAAAAGAGCAGATC
TTTTTATTGGGTAGCGTGTTATCTGTTTACTTTGCTCAATATGCGGAGATTAACGTGTTTACGCAGTTAGAGATTAAGTT
GAAATCAACCAGCAGCTCCTTTCACGTATGGCCTGCGCTGACAGGTGATAAGGTGTTGTTATGA					