SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP012219
Component protein type	FHA
T6SS ID (Type)	T6SS00720 (Type i5)
Strain	Vibrio parahaemolyticus BB22OP
Replicon	chromosome II
Sequence	Protein sequence (399 a.a.); Nucleotide sequence (1197 bp)
Reference	[1] Gode-Potratz CJ, McCarter LL. Quorum sensing and silencing in Vibrio parahaemolyticus. J Bacteriol. 2011 Aug;193(16):4224-37. doi: 10.1128/JB.00432-11. Epub 2011 Jun 24. PMID: 21705592 [2] Fridman CM, Keppel K, Gerlic M, et al. A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors. Nat Commun. 2020 Feb 27;11(1):1085. doi: 10.1038/s41467-020-14951-4. PMID: 32109231

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VPBB_RS20000
Coordinate (Strand)	1070719..1071915 (-)
NCBI ID	WP_015313314.1
RefSeq	NC_019971
Uniprot ID	UPI0002A5834F
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
FHA	PF00498.28	1.2e-14	32..101

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-398	MKLVLTITSFHKFTPEIESEFVFESSDEHAKVTFGRSEQCDWTLPDPERVISGTHGELIKFGDKYLIKDLSTNGTFVNNAVTPIGQGNELALSHGDTVALGDYQILISISDIPEDRTEHQFTSKPEVKTPVEFEQHVESIPEVEDFGLSAAELMDESVPSDFQLDIGLVDDFLEIPSHETEVPQPKVASMQPDTNAKSQKPTVHRGELEAFIYGMGIDPNMVPSQNREQWFEQLGQSFSLMLIGLMETLHNRAVFKQTNRVNHTAFRKSENNPLKFSANLEDAIHNLYNRQTSSFLKPNAAIKEAFNDIENHEQALMQGVAGTVSSVMSLVEPNTIYSDALAKDNVINKVIPARKYAQSWKRFEGIHSQLTDELVNKESPFYLEDFAKHYELALKNKG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9679	-	-

Protein sequence: 399 a.a.

						>T6CP012219 NC_019971:c1071915-1070719 [Vibrio parahaemolyticus BB22OP] [FHA]
MKLVLTITSFHKFTPEIESEFVFESSDEHAKVTFGRSEQCDWTLPDPERVISGTHGELIKFGDKYLIKDLSTNGTFVNNA
VTPIGQGNELALSHGDTVALGDYQILISISDIPEDRTEHQFTSKPEVKTPVEFEQHVESIPEVEDFGLSAAELMDESVPS
DFQLDIGLVDDFLEIPSHETEVPQPKVASMQPDTNAKSQKPTVHRGELEAFIYGMGIDPNMVPSQNREQWFEQLGQSFSL
MLIGLMETLHNRAVFKQTNRVNHTAFRKSENNPLKFSANLEDAIHNLYNRQTSSFLKPNAAIKEAFNDIENHEQALMQGV
AGTVSSVMSLVEPNTIYSDALAKDNVINKVIPARKYAQSWKRFEGIHSQLTDELVNKESPFYLEDFAKHYELALKNKG*					

Nucleotide sequence: 1197 bp

						>T6CP012219 NC_019971:c1071915-1070719 [Vibrio parahaemolyticus BB22OP] [FHA]
ATGAAATTGGTATTGACCATAACGAGTTTCCACAAATTTACTCCGGAAATAGAGAGTGAGTTTGTGTTTGAATCAAGTGA
TGAGCATGCAAAGGTAACCTTCGGACGCTCAGAACAATGTGACTGGACGTTACCAGACCCTGAGAGAGTTATTTCTGGGA
CTCACGGCGAATTGATTAAATTTGGCGATAAATATCTAATTAAAGATTTATCAACAAATGGCACATTTGTTAATAATGCA
GTTACTCCAATCGGCCAAGGGAATGAGCTTGCTTTGTCCCATGGTGATACTGTTGCTTTAGGCGATTATCAAATACTAAT
TAGTATTAGTGATATCCCTGAAGATAGGACAGAGCATCAATTTACATCTAAACCAGAGGTTAAGACGCCAGTTGAATTCG
AACAGCACGTAGAATCGATTCCAGAGGTGGAAGATTTTGGTTTAAGCGCTGCCGAGTTGATGGATGAAAGTGTCCCATCT
GATTTTCAGTTAGACATTGGTCTAGTAGATGATTTTCTAGAAATTCCGAGTCACGAAACTGAAGTGCCACAACCTAAAGT
TGCATCAATGCAGCCTGATACCAACGCTAAAAGTCAAAAACCTACAGTGCATCGAGGTGAACTGGAAGCCTTCATTTATG
GCATGGGGATCGACCCCAATATGGTACCAAGCCAAAATCGAGAGCAGTGGTTTGAACAATTAGGTCAGTCGTTTTCTCTC
ATGCTTATTGGGCTAATGGAGACTCTTCACAATCGGGCTGTATTTAAACAAACCAATCGCGTCAACCATACTGCATTTAG
AAAGTCTGAAAATAATCCACTTAAGTTTTCAGCAAATTTGGAAGATGCAATCCACAATTTGTATAACCGTCAAACGTCAA
GTTTTTTAAAGCCTAATGCCGCAATAAAAGAAGCATTTAACGATATTGAAAATCATGAGCAGGCTTTAATGCAAGGAGTC
GCGGGTACGGTATCGAGTGTCATGTCTCTCGTTGAACCTAATACTATTTATTCAGATGCATTGGCTAAAGACAACGTAAT
TAATAAGGTTATTCCAGCAAGAAAATATGCTCAGAGTTGGAAGCGCTTTGAAGGTATTCATAGCCAATTAACAGATGAAC
TTGTTAATAAAGAGTCTCCATTTTATTTAGAAGATTTCGCTAAGCACTACGAATTAGCGCTCAAGAATAAAGGTTAA					