SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP007575
Component protein type	TssI
T6SS ID (Type)	T6SS00720 (Type i5)
Strain	Vibrio parahaemolyticus BB22OP
Replicon	chromosome II
Sequence	Protein sequence (612 a.a.); Nucleotide sequence (1836 bp)
Reference	[1] Gode-Potratz CJ, McCarter LL. Quorum sensing and silencing in Vibrio parahaemolyticus. J Bacteriol. 2011 Aug;193(16):4224-37. doi: 10.1128/JB.00432-11. Epub 2011 Jun 24. PMID: 21705592 [2] Fridman CM, Keppel K, Gerlic M, et al. A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors. Nat Commun. 2020 Feb 27;11(1):1085. doi: 10.1038/s41467-020-14951-4. PMID: 32109231

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VPBB_RS19915
Coordinate (Strand)	1048650..1050485 (-)
NCBI ID	WP_015313301.1
RefSeq	NC_019971
Uniprot ID	UPI0002A58045
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	9.7e-75	30..335
Phage_base_V	PF04717.14	1.5e-17	381..461

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-611	MKKASQDNNFIRISSPFGKDALILNLFEYREGISELFSLRAKAYFNDQKNELNEIVGKEVTISVENSSRVSKSPRFFHGIVSAAKLEGQRVMNSHNGENYKNIEIIVEPKVKFAAYRNNCKIFQKKNIKDIISEVLSEHGVAFKFELKNTYPQYSYKVQYEESDLAFVQRLLAEEGLSFCFSHSKSSHVLDIFDDVSFYKPSPEFMVDFDTGSSESSHISAWNETQVLTTKSSQKSGFNMLKPASQPKNVAAGDTALFTVPASEYFEYLGETESDDQYSLRNTHAIESLQQNVYLCSGEASCRTFSVGKCFKFKKHEDKSRVGKEYVLASVTIFASVFNQTGLGGTASQGVRVAFTCVDSKTILRPAVTYPKPQIKGLQTAIVTGNKDGEVYVDKHGRIKVQFHWDRLGKYDVNSSCWIRVAQSVAGNGWGAVFHPRVGQEVIVEFVNGDPDQPIVTGALYNGSQLPPYSLPEKSSQSGFKSRSVQKGNANFNELRFEDKPGEEHIYLHAEKLFQMLVEDCVDIVVENNKVEKVTNDVTQDVGKNAMLKVGENYTSDTGKVLSLNAGKSIEIKVGGASIQMSSSGEINIKGNKISINGSAIALKAGQISLN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9836	-	-

Protein sequence: 612 a.a.

						>T6CP007575 NC_019971:c1050485-1048650 [Vibrio parahaemolyticus BB22OP] [TssI]
MKKASQDNNFIRISSPFGKDALILNLFEYREGISELFSLRAKAYFNDQKNELNEIVGKEVTISVENSSRVSKSPRFFHGI
VSAAKLEGQRVMNSHNGENYKNIEIIVEPKVKFAAYRNNCKIFQKKNIKDIISEVLSEHGVAFKFELKNTYPQYSYKVQY
EESDLAFVQRLLAEEGLSFCFSHSKSSHVLDIFDDVSFYKPSPEFMVDFDTGSSESSHISAWNETQVLTTKSSQKSGFNM
LKPASQPKNVAAGDTALFTVPASEYFEYLGETESDDQYSLRNTHAIESLQQNVYLCSGEASCRTFSVGKCFKFKKHEDKS
RVGKEYVLASVTIFASVFNQTGLGGTASQGVRVAFTCVDSKTILRPAVTYPKPQIKGLQTAIVTGNKDGEVYVDKHGRIK
VQFHWDRLGKYDVNSSCWIRVAQSVAGNGWGAVFHPRVGQEVIVEFVNGDPDQPIVTGALYNGSQLPPYSLPEKSSQSGF
KSRSVQKGNANFNELRFEDKPGEEHIYLHAEKLFQMLVEDCVDIVVENNKVEKVTNDVTQDVGKNAMLKVGENYTSDTGK
VLSLNAGKSIEIKVGGASIQMSSSGEINIKGNKISINGSAIALKAGQISLN*					

Nucleotide sequence: 1836 bp

						>T6CP007575 NC_019971:c1050485-1048650 [Vibrio parahaemolyticus BB22OP] [TssI]
ATGAAAAAAGCAAGTCAAGACAACAACTTTATCCGTATTTCTTCTCCGTTTGGAAAAGATGCTCTTATTCTCAATTTGTT
CGAATATAGAGAAGGGATCTCGGAGCTTTTTTCATTGCGTGCTAAAGCTTATTTTAATGATCAAAAAAATGAGCTCAATG
AAATCGTGGGTAAAGAAGTAACGATTTCAGTAGAAAATAGTAGTCGTGTTTCTAAAAGTCCGCGTTTTTTCCATGGCATT
GTCTCTGCTGCAAAGCTCGAAGGCCAGAGAGTGATGAATTCTCATAATGGAGAAAACTACAAAAATATTGAAATTATTGT
TGAACCGAAAGTTAAGTTCGCAGCGTACAGAAATAATTGTAAGATCTTTCAGAAGAAAAATATAAAAGACATTATTTCCG
AAGTGCTTTCAGAGCATGGTGTTGCATTTAAATTTGAACTAAAAAATACGTATCCCCAATATTCCTACAAAGTTCAGTAT
GAAGAATCTGATCTTGCTTTTGTTCAGCGCCTCCTAGCCGAAGAAGGGCTCTCTTTTTGCTTTAGTCACTCTAAATCATC
CCATGTCCTAGATATTTTTGATGATGTAAGTTTTTACAAACCCAGTCCTGAGTTTATGGTCGATTTCGATACAGGCAGTT
CAGAGTCGAGTCACATTTCTGCTTGGAACGAAACACAAGTTTTAACGACTAAATCAAGCCAGAAGTCTGGCTTCAATATG
CTAAAACCAGCCTCACAACCCAAAAACGTAGCTGCTGGTGATACTGCACTGTTTACCGTACCTGCGAGTGAGTATTTTGA
GTATCTGGGTGAAACAGAAAGTGACGATCAGTACTCGCTTCGAAACACTCACGCAATTGAATCCCTTCAACAGAACGTTT
ATCTATGTAGCGGAGAGGCGAGTTGTCGAACCTTTTCTGTGGGTAAATGTTTTAAGTTCAAAAAACACGAAGATAAATCG
CGAGTAGGAAAAGAATATGTCTTGGCCTCTGTGACGATTTTTGCTTCTGTGTTTAATCAAACTGGTCTGGGTGGCACCGC
TTCTCAAGGTGTTCGTGTTGCGTTTACTTGTGTGGACTCTAAAACCATTTTAAGGCCAGCGGTTACTTACCCTAAACCGC
AAATTAAAGGTCTTCAAACAGCCATTGTGACTGGTAATAAAGACGGTGAGGTATACGTTGATAAACATGGAAGAATAAAA
GTACAGTTCCATTGGGATCGTTTGGGTAAGTACGATGTAAATAGTTCGTGTTGGATTCGAGTTGCTCAAAGTGTTGCCGG
AAATGGATGGGGTGCGGTGTTTCATCCTCGGGTAGGGCAAGAGGTTATCGTAGAGTTTGTGAATGGCGACCCAGATCAAC
CTATTGTCACGGGTGCGCTGTATAACGGGAGCCAGTTGCCCCCGTATTCTCTTCCAGAGAAAAGTTCTCAAAGCGGTTTT
AAGTCCCGCTCAGTTCAAAAAGGAAACGCCAATTTCAACGAGTTACGCTTTGAAGATAAACCTGGCGAAGAACACATTTA
CCTTCACGCAGAAAAGTTGTTCCAAATGTTAGTCGAAGACTGCGTGGATATTGTGGTTGAGAACAATAAAGTTGAGAAAG
TAACCAACGATGTAACTCAAGACGTTGGAAAAAATGCGATGTTAAAGGTGGGAGAGAACTATACCTCTGACACTGGAAAA
GTGTTGTCCCTCAATGCAGGGAAAAGTATTGAAATTAAAGTCGGTGGAGCCTCAATTCAAATGTCGAGTTCTGGCGAAAT
CAACATTAAGGGCAACAAGATTTCTATCAATGGCTCTGCAATCGCATTGAAAGCAGGACAAATCTCTTTGAATTAA					