Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   E0E15_RS08265 Genome accession   NZ_LR027870
Coordinates   1634140..1635231 (-) Length   363 a.a.
NCBI ID   WP_130827171.1    Uniprot ID   -
Organism   Staphylococcus aureus strain BPH2019 isolate BPH2019     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1629140..1640231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0E15_RS08220 gcvT 1629373..1630464 (-) 1092 WP_000093351.1 glycine cleavage system aminomethyltransferase GcvT -
  E0E15_RS08225 - 1630623..1631147 (-) 525 WP_116100530.1 shikimate kinase -
  E0E15_RS08230 - 1631137..1631282 (-) 146 Protein_1544 hypothetical protein -
  E0E15_RS08235 comGF 1631379..1631876 (-) 498 WP_001803317.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0E15_RS08240 comGE 1631794..1632093 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  E0E15_RS08245 comGD 1632080..1632526 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0E15_RS08250 comGC 1632504..1632815 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E0E15_RS08260 - 1632984..1634156 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  E0E15_RS08265 comGB 1634140..1635231 (-) 1092 WP_130827171.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0E15_RS08270 comGA 1635203..1636177 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  E0E15_RS08275 - 1636229..1636852 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  E0E15_RS08280 - 1636849..1637178 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E0E15_RS08285 - 1637178..1638164 (-) 987 WP_000161314.1 ROK family glucokinase -
  E0E15_RS08290 - 1638161..1638364 (-) 204 WP_000087561.1 YqgQ family protein -
  E0E15_RS08295 - 1638345..1639808 (-) 1464 WP_001017867.1 rhomboid family protein -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 42201.64 Da        Isoelectric Point: 10.0088

>NTDB_id=993040 E0E15_RS08265 WP_130827171.1 1634140..1635231(-) (comGB) [Staphylococcus aureus strain BPH2019 isolate BPH2019]
MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKILSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVKSRLLCKMLYNVFTISTYQN

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=993040 E0E15_RS08265 WP_130827171.1 1634140..1635231(-) (comGB) [Staphylococcus aureus strain BPH2019 isolate BPH2019]
GTGAAACTACAATGCATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGACTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATCTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAATATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTCAAGTCCAGACT
CCTGTGTAAAATGCTATACAATGTTTTTACCATTTCTACTTATCAAAATTGA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)

Type II secretion system (T2SS), protein F (T2SSF)

(227-343)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.417

94.49

0.939
  comGB Staphylococcus aureus N315

99.417

94.49

0.939

Multiple sequence alignment