Detailed information    

insolico Bioinformatically predicted

Overview

Name   recA   Type   Machinery gene
Locus tag   WLF14_RS06300 Genome accession   NZ_CP151816
Coordinates   1387878..1388936 (+) Length   352 a.a.
NCBI ID   WP_024073752.1    Uniprot ID   A0AB36YB49
Organism   Pseudomonas fluorescens strain IMGN2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1374014..1388936 1387878..1388936 within 0


Gene organization within MGE regions


Location: 1374014..1388936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WLF14_RS06195 (WLF14_06195) - 1374014..1374748 (+) 735 WP_024073730.1 XRE family transcriptional regulator -
  WLF14_RS06200 (WLF14_06200) - 1375343..1375687 (+) 345 WP_168232394.1 phage holin family protein -
  WLF14_RS06205 (WLF14_06205) - 1375709..1376224 (+) 516 WP_024073733.1 hypothetical protein -
  WLF14_RS06210 (WLF14_06210) - 1376228..1376839 (+) 612 WP_024073734.1 phage baseplate assembly protein V -
  WLF14_RS06215 (WLF14_06215) - 1376849..1377181 (+) 333 WP_024073735.1 phage baseplate protein -
  WLF14_RS06220 (WLF14_06220) - 1377178..1378173 (+) 996 WP_024073736.1 baseplate J/gp47 family protein -
  WLF14_RS06225 (WLF14_06225) - 1378170..1378799 (+) 630 WP_342309113.1 phage tail protein I -
  WLF14_RS06230 (WLF14_06230) - 1378800..1380251 (+) 1452 WP_342309115.1 phage tail protein -
  WLF14_RS06235 (WLF14_06235) - 1380253..1380660 (+) 408 WP_342309116.1 hypothetical protein -
  WLF14_RS06240 (WLF14_06240) - 1380759..1380968 (+) 210 WP_342309117.1 hypothetical protein -
  WLF14_RS06245 (WLF14_06245) - 1380968..1382134 (+) 1167 WP_342309119.1 phage tail protein -
  WLF14_RS06250 (WLF14_06250) - 1382134..1382640 (+) 507 WP_342309120.1 phage major tail tube protein -
  WLF14_RS06255 (WLF14_06255) - 1382690..1383262 (+) 573 WP_342309122.1 phage tail assembly protein -
  WLF14_RS06260 (WLF14_06260) - 1383259..1383384 (+) 126 WP_024073744.1 hypothetical protein -
  WLF14_RS06265 (WLF14_06265) - 1383391..1384536 (+) 1146 WP_342309124.1 hypothetical protein -
  WLF14_RS06270 (WLF14_06270) - 1384536..1384919 (+) 384 WP_024073746.1 phage tail protein -
  WLF14_RS06275 (WLF14_06275) - 1384912..1385124 (+) 213 WP_069075367.1 tail protein X -
  WLF14_RS06280 (WLF14_06280) - 1385134..1386144 (+) 1011 WP_069075368.1 contractile injection system protein, VgrG/Pvc8 family -
  WLF14_RS06285 (WLF14_06285) - 1386167..1386724 (+) 558 WP_024073749.1 glycoside hydrolase family 19 protein -
  WLF14_RS06290 (WLF14_06290) - 1386712..1387212 (+) 501 WP_024073750.1 lysis system i-spanin subunit Rz -
  WLF14_RS06295 (WLF14_06295) - 1387294..1387794 (+) 501 WP_024073751.1 nicotinamide-nucleotide amidohydrolase family protein -
  WLF14_RS06300 (WLF14_06300) recA 1387878..1388936 (+) 1059 WP_024073752.1 recombinase RecA Machinery gene

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37721.29 Da        Isoelectric Point: 5.3036

>NTDB_id=980997 WLF14_RS06300 WP_024073752.1 1387878..1388936(+) (recA) [Pseudomonas fluorescens strain IMGN2]
MDDNKKKALAAALGQIERQFGKGAVMRMGDHDRQAIPAISTGSLGLDIALGIGGLPKGRIVEIYGPESSGKTTLTLSVIA
QAQKMGATCAFVDAEHALDPEYAGKLGVNVDDLLVSQPDTGEQALEITDMLVRSNAIDVIVVDSVAALVPKAEIEGEMGD
MHVGLQARLMSQALRKITGNIKNANCLVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRTGAVKEGDEVVGSE
TRVKVVKNKVAPPFRQAEFQILYGKGIYLNGEMIDLGVLHGFVEKSGAWYAYNGSKIGQGKANSAKFLQDNPDIAATLEK
QIRDKLLTPTPDVKAAANREPVEEVEEVDTDI

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=980997 WLF14_RS06300 WP_024073752.1 1387878..1388936(+) (recA) [Pseudomonas fluorescens strain IMGN2]
ATGGACGACAACAAGAAGAAAGCCTTGGCTGCGGCCCTGGGTCAGATCGAACGTCAATTCGGCAAGGGTGCCGTAATGCG
TATGGGCGATCATGACCGTCAGGCGATCCCGGCTATCTCTACTGGCTCTCTGGGTCTGGACATCGCGCTCGGCATTGGCG
GCCTGCCAAAAGGCCGTATCGTTGAAATCTACGGTCCTGAATCTTCCGGTAAAACCACCCTGACCTTGTCGGTGATTGCC
CAGGCGCAAAAAATGGGCGCCACTTGCGCGTTCGTCGACGCCGAGCACGCCCTTGACCCTGAATATGCCGGCAAGCTGGG
CGTCAACGTTGACGACCTGCTGGTTTCCCAACCGGACACCGGTGAGCAAGCCCTCGAAATCACCGACATGCTGGTGCGCT
CCAACGCCATCGACGTGATCGTGGTCGACTCCGTGGCTGCCCTGGTACCGAAGGCTGAAATCGAAGGCGAAATGGGTGAT
ATGCACGTGGGCCTGCAAGCCCGCCTCATGTCCCAGGCGCTGCGTAAAATCACCGGTAACATCAAGAATGCCAACTGCCT
GGTGATCTTCATCAACCAGATCCGGATGAAGATCGGCGTCATGTTCGGCAGCCCGGAAACCACCACCGGTGGTAACGCGC
TGAAGTTCTACGCTTCGGTTCGTCTGGATATCCGTCGTACTGGCGCGGTGAAGGAAGGTGACGAGGTTGTCGGTAGCGAA
ACCCGCGTCAAGGTCGTGAAGAATAAAGTGGCCCCGCCATTCCGTCAGGCCGAGTTCCAGATTCTCTACGGCAAGGGTAT
CTACCTGAACGGCGAGATGATCGACCTGGGCGTGCTGCACGGCTTTGTCGAGAAGTCCGGTGCCTGGTATGCCTACAACG
GCAGCAAAATCGGCCAGGGCAAGGCCAACTCGGCCAAGTTCCTGCAAGACAACCCGGATATCGCGGCGACCCTTGAGAAG
CAGATTCGCGACAAGCTGCTGACCCCTACGCCAGACGTGAAAGCGGCCGCCAATCGCGAGCCGGTTGAAGAAGTAGAAGA
AGTCGACACTGACATCTGA

Domains

Predicted by InterproScan.

recA bacterial DNA recombination protein (RecA)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Pseudomonas stutzeri DSM 10701

87.216

100

0.872
  recA Acinetobacter baylyi ADP1

74.709

97.727

0.73
  recA Acinetobacter baumannii D1279779

75.61

93.182

0.705
  recA Vibrio cholerae O1 biovar El Tor strain E7946

75.076

93.466

0.702
  recA Vibrio cholerae strain A1552

75.076

93.466

0.702
  recA Ralstonia pseudosolanacearum GMI1000

68.347

100

0.693
  recA Glaesserella parasuis strain SC1401

69.429

99.432

0.69
  recA Neisseria gonorrhoeae MS11

72.222

92.045

0.665
  recA Neisseria gonorrhoeae MS11

72.222

92.045

0.665
  recA Neisseria gonorrhoeae strain FA1090

72.222

92.045

0.665
  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

64.526

92.898

0.599
  recA Helicobacter pylori strain NCTC11637

59.943

100

0.599
  recA Helicobacter pylori 26695

59.375

100

0.594
  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

61.677

94.886

0.585
  recA Bacillus subtilis subsp. subtilis str. 168

61.682

91.193

0.562
  recA Streptococcus mutans UA159

59.692

92.33

0.551
  recA Streptococcus mitis SK321

54.83

100

0.548
  recA Streptococcus pyogenes NZ131

57.927

93.182

0.54
  recA Streptococcus pneumoniae TIGR4

58.282

92.614

0.54
  recA Streptococcus pneumoniae R6

58.282

92.614

0.54
  recA Streptococcus pneumoniae Rx1

58.282

92.614

0.54
  recA Streptococcus pneumoniae D39

58.282

92.614

0.54
  recA Streptococcus mitis NCTC 12261

58.514

91.761

0.537
  recA Latilactobacillus sakei subsp. sakei 23K

58.567

91.193

0.534
  recA Lactococcus lactis subsp. cremoris KW2

57.276

91.761

0.526
  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.481

92.045

0.52

Multiple sequence alignment