Detailed information    

insolico Bioinformatically predicted

Overview


Name   recA   Type   Machinery gene
Locus tag   WIR89_RS11745 Genome accession   NZ_CP149711
Coordinates   2628162..2629208 (-) Length   348 a.a.
NCBI ID   WP_021364420.1    Uniprot ID   A0A9X8RFQ1
Organism   Clostridioides difficile strain Z1322PCD0001     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2581913..2647564 2628162..2629208 within 0


Gene organization within MGE regions


Location: 2581913..2647564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WIR89_RS11520 (WIR89_11520) - 2582564..2583262 (-) 699 WP_003438451.1 putative phage tail protein -
  WIR89_RS11525 (WIR89_11525) - 2583259..2584311 (-) 1053 WP_003438450.1 baseplate J/gp47 family protein -
  WIR89_RS11530 (WIR89_11530) - 2584304..2584732 (-) 429 WP_003438449.1 DUF2634 domain-containing protein -
  WIR89_RS11535 (WIR89_11535) - 2584732..2585058 (-) 327 WP_003438447.1 DUF2577 domain-containing protein -
  WIR89_RS11540 (WIR89_11540) - 2585074..2586603 (-) 1530 WP_009889145.1 NlpC/P60 family protein -
  WIR89_RS11545 (WIR89_11545) - 2586885..2587307 (-) 423 WP_003428168.1 phage baseplate protein -
  WIR89_RS11550 (WIR89_11550) - 2587307..2589760 (-) 2454 WP_003438437.1 hypothetical protein -
  WIR89_RS11555 (WIR89_11555) - 2589760..2589927 (-) 168 WP_003419648.1 hypothetical protein -
  WIR89_RS11560 (WIR89_11560) - 2589954..2590400 (-) 447 WP_009902432.1 phage portal protein -
  WIR89_RS11565 (WIR89_11565) - 2590526..2590954 (-) 429 WP_003419646.1 phage tail tube protein -
  WIR89_RS11570 (WIR89_11570) - 2590968..2592032 (-) 1065 WP_011861188.1 phage tail sheath subtilisin-like domain-containing protein -
  WIR89_RS11575 (WIR89_11575) - 2592037..2592480 (-) 444 WP_009896258.1 DUF6838 family protein -
  WIR89_RS11580 (WIR89_11580) - 2592729..2593169 (-) 441 WP_003428183.1 hypothetical protein -
  WIR89_RS11585 (WIR89_11585) - 2593274..2593471 (-) 198 WP_011861187.1 helix-turn-helix transcriptional regulator -
  WIR89_RS11590 (WIR89_11590) - 2593640..2593960 (+) 321 WP_003428185.1 XRE family transcriptional regulator -
  WIR89_RS11595 (WIR89_11595) - 2594042..2594548 (+) 507 WP_011861186.1 hypothetical protein -
  WIR89_RS11600 (WIR89_11600) - 2595249..2596004 (+) 756 WP_009889131.1 peptidylprolyl isomerase -
  WIR89_RS11605 (WIR89_11605) - 2596147..2596575 (-) 429 WP_009889129.1 CBS domain-containing protein -
  WIR89_RS11610 (WIR89_11610) - 2596996..2597196 (-) 201 WP_003419624.1 cold-shock protein -
  WIR89_RS11615 (WIR89_11615) - 2597379..2598533 (-) 1155 WP_009902426.1 amidase domain-containing protein -
  WIR89_RS11620 (WIR89_11620) - 2598784..2599605 (+) 822 WP_003428191.1 pyridoxamine kinase -
  WIR89_RS11625 (WIR89_11625) - 2599681..2601069 (-) 1389 WP_011861185.1 two-component system sensor histidine kinase -
  WIR89_RS11630 (WIR89_11630) - 2601204..2601962 (-) 759 WP_021421479.1 lantibiotic immunity ABC transporter MutG family permease subunit -
  WIR89_RS11635 (WIR89_11635) - 2601964..2602680 (-) 717 WP_016729591.1 lantibiotic immunity ABC transporter MutE/EpiE family permease subunit -
  WIR89_RS11640 (WIR89_11640) - 2602682..2603389 (-) 708 WP_021363440.1 lantibiotic protection ABC transporter ATP-binding protein -
  WIR89_RS11645 (WIR89_11645) - 2603505..2604041 (-) 537 WP_003428196.1 lipoprotein -
  WIR89_RS11650 (WIR89_11650) - 2604143..2605213 (-) 1071 WP_009896243.1 DUF4097 family beta strand repeat-containing protein -
  WIR89_RS11655 (WIR89_11655) - 2605206..2606264 (-) 1059 WP_021367719.1 DUF1700 domain-containing protein -
  WIR89_RS11660 (WIR89_11660) - 2606251..2606583 (-) 333 WP_009889116.1 PadR family transcriptional regulator -
  WIR89_RS11665 (WIR89_11665) - 2606782..2607903 (+) 1122 WP_003438374.1 glycosyltransferase -
  WIR89_RS11670 (WIR89_11670) - 2608031..2609929 (-) 1899 WP_003428202.1 glutamine synthetase -
  WIR89_RS11675 (WIR89_11675) - 2610244..2611020 (+) 777 WP_009896237.1 3'-5' exonuclease -
  WIR89_RS11680 (WIR89_11680) - 2611112..2611864 (-) 753 WP_003438367.1 exodeoxyribonuclease III -
  WIR89_RS11685 (WIR89_11685) galT 2612025..2612975 (-) 951 WP_009889113.1 galactose-1-phosphate uridylyltransferase -
  WIR89_RS11690 (WIR89_11690) - 2613360..2614556 (+) 1197 WP_074105864.1 pyridoxal phosphate-dependent aminotransferase -
  WIR89_RS11695 (WIR89_11695) - 2614668..2616014 (-) 1347 WP_009902417.1 6-phospho-alpha-glucosidase -
  WIR89_RS11700 (WIR89_11700) - 2616147..2616899 (-) 753 WP_009892977.1 MurR/RpiR family transcriptional regulator -
  WIR89_RS11705 (WIR89_11705) - 2617047..2618624 (-) 1578 WP_009902415.1 PTS transporter subunit EIIC -
  WIR89_RS11710 (WIR89_11710) - 2619036..2619488 (-) 453 WP_003419561.1 MarR family transcriptional regulator -
  WIR89_RS11715 (WIR89_11715) purB 2619799..2621244 (-) 1446 WP_003438353.1 adenylosuccinate lyase -
  WIR89_RS11720 (WIR89_11720) - 2621388..2622296 (-) 909 WP_009889110.1 tyrosine-type recombinase/integrase -
  WIR89_RS11725 (WIR89_11725) - 2622553..2623377 (+) 825 WP_074105863.1 undecaprenyl-diphosphate phosphatase -
  WIR89_RS11730 (WIR89_11730) lepB 2623417..2623947 (+) 531 WP_009902411.1 signal peptidase I -
  WIR89_RS11735 (WIR89_11735) - 2624029..2625810 (-) 1782 WP_003438344.1 UvrD-helicase domain-containing protein -
  WIR89_RS11740 (WIR89_11740) rny 2626445..2627986 (-) 1542 WP_003428216.1 ribonuclease Y -
  WIR89_RS11745 (WIR89_11745) recA 2628162..2629208 (-) 1047 WP_021364420.1 recombinase RecA Machinery gene
  WIR89_RS11750 (WIR89_11750) pgsA 2629496..2630038 (-) 543 WP_009889106.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  WIR89_RS11755 (WIR89_11755) rimO 2630025..2631359 (-) 1335 WP_003433320.1 30S ribosomal protein S12 methylthiotransferase RimO -
  WIR89_RS11760 (WIR89_11760) mnmH 2631353..2632411 (-) 1059 WP_003433322.1 tRNA 2-selenouridine(34) synthase MnmH -
  WIR89_RS11765 (WIR89_11765) - 2632414..2634825 (-) 2412 WP_003438328.1 DNA translocase FtsK -
  WIR89_RS11770 (WIR89_11770) - 2634948..2635682 (-) 735 WP_021364422.1 ATP-dependent Clp protease proteolytic subunit -
  WIR89_RS11775 (WIR89_11775) dapG 2635795..2637018 (-) 1224 WP_003438324.1 aspartate kinase -
  WIR89_RS11780 (WIR89_11780) - 2637028..2637273 (-) 246 WP_021364423.1 YlmC/YmxH family sporulation protein -
  WIR89_RS11785 (WIR89_11785) - 2637308..2638555 (-) 1248 WP_009902409.1 pitrilysin family protein -
  WIR89_RS11790 (WIR89_11790) - 2638713..2639456 (-) 744 WP_003438319.1 polysaccharide deacetylase family protein -
  WIR89_RS11795 (WIR89_11795) pnp 2639722..2641833 (-) 2112 WP_003438316.1 polyribonucleotide nucleotidyltransferase -
  WIR89_RS11800 (WIR89_11800) - 2641958..2642803 (-) 846 WP_009889099.1 DegV family protein -
  WIR89_RS11805 (WIR89_11805) rpsO 2643043..2643300 (-) 258 WP_003419491.1 30S ribosomal protein S15 -
  WIR89_RS11810 (WIR89_11810) - 2643404..2644333 (-) 930 WP_003438310.1 bifunctional riboflavin kinase/FAD synthetase -
  WIR89_RS11815 (WIR89_11815) truB 2644464..2645369 (-) 906 WP_003428232.1 tRNA pseudouridine(55) synthase TruB -
  WIR89_RS11820 (WIR89_11820) - 2645382..2645687 (-) 306 Protein_2353 DHHA1 domain-containing protein -
  WIR89_RS11825 (WIR89_11825) tnpB 2645805..2646923 (-) 1119 WP_032548186.1 IS200/IS605 family element RNA-guided endonuclease TnpB -

Sequence


Protein


Download         Length: 348 a.a.        Molecular weight: 37389.74 Da        Isoelectric Point: 4.8236

>NTDB_id=960916 WIR89_RS11745 WP_021364420.1 2628162..2629208(-) (recA) [Clostridioides difficile strain Z1322PCD0001]
MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTGAIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSC
VASAQKDGGIAAFIDAEHALDPVYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKFYSSVRLDVRKIDTIKQGDKVIG
SRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLLDIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEI
DEKVRAFYNLNEEHEESGISVSKEIVEE

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=960916 WIR89_RS11745 WP_021364420.1 2628162..2629208(-) (recA) [Clostridioides difficile strain Z1322PCD0001]
ATGAGTGTAGATCAAGAAAAATTAAAAGCGTTGAATGAAGCTTTAGGTAAAATTGAAAAAGATTTTGGTAAAGGTTCAGT
AATGAAATTGGGAGAAGCAACATCTATGTCTATAGATGTTATATCAACAGGAGCGATTGGTTTAGACATAGCTATTGGTA
TAGGAGGTCTACCTAGAGGGAGAATAGTTGAAGTATATGGTCCAGAATCTTCTGGTAAAACTACTGTTGCGCTTAGTTGT
GTAGCATCAGCTCAAAAAGATGGAGGAATAGCTGCATTTATAGATGCAGAACATGCACTTGACCCAGTATATGCAAAAGC
TTTGGGTGTGGATGTTGATAACCTAATAATATCTCAACCAGATACAGGTGAACAGGCTTTAGAGATAGCAGAGGCATTGA
TAAGAAGTGGAGCGATAGATATAATAGTAATAGACTCAGTAGCAGCATTAGTTCCAAAGGCTGAAATAGATGGAGATATG
GGTGATTCTCATGTAGGTTTACAAGCTAGACTTATGTCACAAGCACTTAGAAAGTTAACTGGTTCAATTAAAAAATCAAA
TTGTGTTGCTATATTTATAAACCAGTTAAGAGAGAAAGTAGGAATAATGTTTGGAAACCCGGAAACTACTACTGGAGGAC
GTGCACTAAAATTCTATTCATCAGTTAGATTGGATGTTAGAAAAATAGATACAATAAAACAAGGTGATAAAGTTATAGGT
AGTAGAACTAGAGTTAAAGTTGTTAAAAACAAAGTAGCACCACCATTTAAGCAAGCTGAATTTGATATAATGTATGGAGA
AGGAATTTCAAAAATTGGAGACCTTTTAGATATAGCTGCTGATGTAGATATAGTTAAAAAATCAGGTTCATGGTATAGTT
ACAATGATACTAAACTTGGACAAGGAAGAGAAAATGTTAAAAAATTCTTGGAAGATAATTTAGATTTAACTACTGAAATA
GATGAGAAAGTTAGAGCATTTTACAATTTAAATGAAGAACATGAAGAATCAGGTATTTCAGTATCAAAAGAAATTGTAGA
AGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Streptococcus mutans UA159

67.055

98.563

0.661

  recA Streptococcus mitis SK321

67.456

97.126

0.655

  recA Streptococcus mitis NCTC 12261

68.085

94.54

0.644

  recA Bacillus subtilis subsp. subtilis str. 168

69.565

92.529

0.644

  recA Streptococcus pneumoniae Rx1

67.173

94.54

0.635

  recA Streptococcus pneumoniae TIGR4

67.173

94.54

0.635

  recA Streptococcus pneumoniae R6

67.173

94.54

0.635

  recA Streptococcus pneumoniae D39

67.173

94.54

0.635

  recA Lactococcus lactis subsp. cremoris KW2

65.569

95.977

0.629

  recA Streptococcus pyogenes NZ131

66.972

93.966

0.629

  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

65.758

94.828

0.624

  recA Latilactobacillus sakei subsp. sakei 23K

64.955

95.115

0.618

  recA Neisseria gonorrhoeae MS11

66.987

89.655

0.601

  recA Neisseria gonorrhoeae MS11

66.987

89.655

0.601

  recA Neisseria gonorrhoeae strain FA1090

66.987

89.655

0.601

  recA Acinetobacter baylyi ADP1

64.396

92.816

0.598

  recA Pseudomonas stutzeri DSM 10701

63.889

93.103

0.595

  recA Helicobacter pylori strain NCTC11637

61.976

95.977

0.595

  recA Helicobacter pylori 26695

61.976

95.977

0.595

  recA Acinetobacter baumannii D1279779

63.777

92.816

0.592

  recA Vibrio cholerae O1 biovar El Tor strain E7946

62.048

95.402

0.592

  recA Vibrio cholerae strain A1552

62.048

95.402

0.592

  recA Glaesserella parasuis strain SC1401

64.174

92.241

0.592

  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

63.272

93.103

0.589

  recA Ralstonia pseudosolanacearum GMI1000

66.558

88.506

0.589

  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

60.681

92.816

0.563