Detailed information    

insolico Bioinformatically predicted

Overview


Name   recA   Type   Machinery gene
Locus tag   WIR68_RS06965 Genome accession   NZ_CP149698
Coordinates   1445410..1446456 (+) Length   348 a.a.
NCBI ID   WP_021364420.1    Uniprot ID   A0A9X8RFQ1
Organism   Clostridioides difficile strain Z1322PCD0028     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1420028..1484196 1445410..1446456 within 0


Gene organization within MGE regions


Location: 1420028..1484196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WIR68_RS06850 (WIR68_06850) rimP 1422444..1422905 (+) 462 WP_003438303.1 ribosome maturation factor RimP -
  WIR68_RS06855 (WIR68_06855) nusA 1422935..1424089 (+) 1155 WP_003428238.1 transcription termination factor NusA -
  WIR68_RS06860 (WIR68_06860) - 1424096..1424377 (+) 282 WP_003419469.1 YlxR family protein -
  WIR68_RS06865 (WIR68_06865) - 1424367..1424678 (+) 312 WP_003419470.1 ribosomal L7Ae/L30e/S12e/Gadd45 family protein -
  WIR68_RS06870 (WIR68_06870) infB 1424700..1426640 (+) 1941 WP_003438306.1 translation initiation factor IF-2 -
  WIR68_RS06875 (WIR68_06875) rbfA 1426683..1427063 (+) 381 WP_003428234.1 30S ribosome-binding factor RbfA -
  WIR68_RS06880 (WIR68_06880) - 1427044..1427721 (+) 678 Protein_1263 DHH family phosphoesterase -
  WIR68_RS06885 (WIR68_06885) tnpB 1428361..1429479 (+) 1119 WP_032548186.1 IS200/IS605 family element RNA-guided endonuclease TnpB -
  WIR68_RS06890 (WIR68_06890) - 1429597..1429902 (+) 306 Protein_1265 DHHA1 domain-containing protein -
  WIR68_RS06895 (WIR68_06895) truB 1429915..1430820 (+) 906 WP_003428232.1 tRNA pseudouridine(55) synthase TruB -
  WIR68_RS06900 (WIR68_06900) - 1430951..1431880 (+) 930 WP_003438310.1 bifunctional riboflavin kinase/FAD synthetase -
  WIR68_RS06905 (WIR68_06905) rpsO 1431984..1432241 (+) 258 WP_003419491.1 30S ribosomal protein S15 -
  WIR68_RS06910 (WIR68_06910) - 1432481..1433326 (+) 846 WP_009889099.1 DegV family protein -
  WIR68_RS06915 (WIR68_06915) pnp 1433451..1435562 (+) 2112 WP_003438316.1 polyribonucleotide nucleotidyltransferase -
  WIR68_RS06920 (WIR68_06920) - 1435828..1436571 (+) 744 WP_003438319.1 polysaccharide deacetylase family protein -
  WIR68_RS06925 (WIR68_06925) - 1436729..1437976 (+) 1248 WP_009902409.1 pitrilysin family protein -
  WIR68_RS06930 (WIR68_06930) - 1438011..1438256 (+) 246 WP_021364423.1 YlmC/YmxH family sporulation protein -
  WIR68_RS06935 (WIR68_06935) dapG 1438266..1439489 (+) 1224 WP_003438324.1 aspartate kinase -
  WIR68_RS06940 (WIR68_06940) - 1439602..1440336 (+) 735 WP_021364422.1 ATP-dependent Clp protease proteolytic subunit -
  WIR68_RS06945 (WIR68_06945) - 1440459..1442870 (+) 2412 WP_003438328.1 DNA translocase FtsK -
  WIR68_RS06950 (WIR68_06950) mnmH 1442873..1443931 (+) 1059 WP_003433322.1 tRNA 2-selenouridine(34) synthase MnmH -
  WIR68_RS06955 (WIR68_06955) - 1443925..1444593 (+) 669 WP_021402035.1 TRAM domain-containing protein -
  WIR68_RS06960 (WIR68_06960) pgsA 1444580..1445122 (+) 543 WP_009889106.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  WIR68_RS06965 (WIR68_06965) recA 1445410..1446456 (+) 1047 WP_021364420.1 recombinase RecA Machinery gene
  WIR68_RS06970 (WIR68_06970) rny 1446632..1448173 (+) 1542 WP_003428216.1 ribonuclease Y -
  WIR68_RS06975 (WIR68_06975) - 1448808..1450589 (+) 1782 WP_003438344.1 UvrD-helicase domain-containing protein -
  WIR68_RS06980 (WIR68_06980) lepB 1450671..1451201 (-) 531 WP_009902411.1 signal peptidase I -
  WIR68_RS06985 (WIR68_06985) - 1451241..1452065 (-) 825 WP_003438350.1 undecaprenyl-diphosphate phosphatase -
  WIR68_RS06990 (WIR68_06990) - 1452322..1453230 (+) 909 WP_009889110.1 tyrosine-type recombinase/integrase -
  WIR68_RS06995 (WIR68_06995) purB 1453374..1454819 (+) 1446 WP_003438353.1 adenylosuccinate lyase -
  WIR68_RS07000 (WIR68_07000) - 1455130..1455582 (+) 453 WP_003419561.1 MarR family transcriptional regulator -
  WIR68_RS07005 (WIR68_07005) - 1455994..1457571 (+) 1578 WP_021364419.1 PTS transporter subunit EIIC -
  WIR68_RS07010 (WIR68_07010) - 1457719..1458471 (+) 753 WP_009892977.1 MurR/RpiR family transcriptional regulator -
  WIR68_RS07015 (WIR68_07015) - 1458604..1459950 (+) 1347 WP_009902417.1 6-phospho-alpha-glucosidase -
  WIR68_RS07020 (WIR68_07020) - 1460062..1461258 (-) 1197 WP_003438357.1 pyridoxal phosphate-dependent aminotransferase -
  WIR68_RS07025 (WIR68_07025) galT 1461643..1462593 (+) 951 WP_009889113.1 galactose-1-phosphate uridylyltransferase -
  WIR68_RS07030 (WIR68_07030) - 1462754..1463506 (+) 753 WP_003438367.1 exodeoxyribonuclease III -
  WIR68_RS07035 (WIR68_07035) - 1463598..1464374 (-) 777 WP_009896237.1 3'-5' exonuclease -
  WIR68_RS07040 (WIR68_07040) - 1464689..1466587 (+) 1899 WP_003428202.1 glutamine synthetase -
  WIR68_RS07045 (WIR68_07045) - 1466715..1467836 (-) 1122 WP_003438374.1 glycosyltransferase -
  WIR68_RS07050 (WIR68_07050) - 1468035..1468367 (+) 333 WP_009889116.1 PadR family transcriptional regulator -
  WIR68_RS07055 (WIR68_07055) - 1468354..1469412 (+) 1059 WP_021367719.1 DUF1700 domain-containing protein -
  WIR68_RS07060 (WIR68_07060) - 1469405..1470475 (+) 1071 WP_021364425.1 DUF4097 family beta strand repeat-containing protein -
  WIR68_RS07065 (WIR68_07065) - 1470577..1471113 (+) 537 WP_003428196.1 lipoprotein -
  WIR68_RS07070 (WIR68_07070) - 1471229..1471936 (+) 708 WP_009902422.1 lantibiotic protection ABC transporter ATP-binding protein -
  WIR68_RS07075 (WIR68_07075) - 1471938..1472654 (+) 717 WP_009896246.1 lantibiotic immunity ABC transporter MutE/EpiE family permease subunit -
  WIR68_RS07080 (WIR68_07080) - 1472656..1473414 (+) 759 WP_009902423.1 lantibiotic immunity ABC transporter MutG family permease subunit -
  WIR68_RS07085 (WIR68_07085) - 1473549..1474937 (+) 1389 WP_021364432.1 two-component system sensor histidine kinase -
  WIR68_RS07090 (WIR68_07090) - 1475013..1475834 (-) 822 WP_021364435.1 pyridoxamine kinase -
  WIR68_RS07095 (WIR68_07095) - 1476085..1477239 (+) 1155 WP_009902426.1 amidase domain-containing protein -
  WIR68_RS07100 (WIR68_07100) - 1477422..1477622 (+) 201 WP_003419624.1 cold-shock protein -
  WIR68_RS07105 (WIR68_07105) - 1478043..1478471 (+) 429 WP_009889129.1 CBS domain-containing protein -
  WIR68_RS07110 (WIR68_07110) - 1478614..1479369 (-) 756 WP_009889131.1 peptidylprolyl isomerase -
  WIR68_RS07115 (WIR68_07115) - 1480070..1480576 (-) 507 WP_003428186.1 hypothetical protein -
  WIR68_RS07120 (WIR68_07120) - 1480658..1480978 (-) 321 WP_003428185.1 XRE family transcriptional regulator -
  WIR68_RS07125 (WIR68_07125) - 1481147..1481344 (+) 198 WP_003419641.1 helix-turn-helix transcriptional regulator -
  WIR68_RS07130 (WIR68_07130) - 1481449..1481889 (+) 441 WP_003428183.1 hypothetical protein -
  WIR68_RS07135 (WIR68_07135) - 1482138..1482581 (+) 444 WP_003438413.1 DUF6838 family protein -
  WIR68_RS07140 (WIR68_07140) - 1482586..1483650 (+) 1065 WP_003438414.1 phage tail sheath subtilisin-like domain-containing protein -
  WIR68_RS07145 (WIR68_07145) - 1483664..1484092 (+) 429 WP_003419646.1 phage tail tube protein -

Sequence


Protein


Download         Length: 348 a.a.        Molecular weight: 37389.74 Da        Isoelectric Point: 4.8236

>NTDB_id=960796 WIR68_RS06965 WP_021364420.1 1445410..1446456(+) (recA) [Clostridioides difficile strain Z1322PCD0028]
MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTGAIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSC
VASAQKDGGIAAFIDAEHALDPVYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKFYSSVRLDVRKIDTIKQGDKVIG
SRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLLDIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEI
DEKVRAFYNLNEEHEESGISVSKEIVEE

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=960796 WIR68_RS06965 WP_021364420.1 1445410..1446456(+) (recA) [Clostridioides difficile strain Z1322PCD0028]
ATGAGTGTAGATCAAGAAAAATTAAAAGCGTTGAATGAAGCTTTAGGTAAAATTGAAAAAGATTTTGGTAAAGGTTCAGT
AATGAAATTGGGAGAAGCAACATCTATGTCTATAGATGTTATATCAACAGGAGCGATTGGTTTAGACATAGCTATTGGTA
TAGGAGGTCTACCTAGAGGGAGAATAGTTGAAGTATATGGTCCAGAATCTTCTGGTAAAACTACTGTTGCGCTTAGTTGT
GTAGCATCAGCTCAAAAAGATGGAGGAATAGCTGCATTTATAGATGCAGAACATGCACTTGACCCAGTATATGCAAAAGC
TTTGGGTGTGGATGTTGATAACCTAATAATATCTCAACCAGATACAGGTGAACAGGCTTTAGAGATAGCAGAGGCATTGA
TAAGAAGTGGAGCGATAGATATAATAGTAATAGACTCAGTAGCAGCATTAGTTCCAAAGGCTGAAATAGATGGAGATATG
GGTGATTCTCATGTAGGTTTACAAGCTAGACTTATGTCACAAGCACTTAGAAAGTTAACTGGTTCAATTAAAAAATCAAA
TTGTGTTGCTATATTTATAAACCAGTTAAGAGAGAAAGTAGGAATAATGTTTGGAAACCCGGAAACTACTACTGGAGGAC
GTGCACTAAAATTCTATTCATCAGTTAGATTGGATGTTAGAAAAATAGATACAATAAAACAAGGTGATAAAGTTATAGGT
AGTAGAACTAGAGTTAAAGTTGTTAAAAACAAAGTAGCACCACCATTTAAGCAAGCTGAATTTGATATAATGTATGGAGA
AGGAATTTCAAAAATTGGAGACCTTTTAGATATAGCTGCTGATGTAGATATAGTTAAAAAATCAGGTTCATGGTATAGTT
ACAATGATACTAAACTTGGACAAGGAAGAGAAAATGTTAAAAAATTCTTGGAAGATAATTTAGATTTAACTACTGAAATA
GATGAGAAAGTTAGAGCATTTTACAATTTAAATGAAGAACATGAAGAATCAGGTATTTCAGTATCAAAAGAAATTGTAGA
AGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Streptococcus mutans UA159

67.055

98.563

0.661

  recA Streptococcus mitis SK321

67.456

97.126

0.655

  recA Streptococcus mitis NCTC 12261

68.085

94.54

0.644

  recA Bacillus subtilis subsp. subtilis str. 168

69.565

92.529

0.644

  recA Streptococcus pneumoniae Rx1

67.173

94.54

0.635

  recA Streptococcus pneumoniae TIGR4

67.173

94.54

0.635

  recA Streptococcus pneumoniae R6

67.173

94.54

0.635

  recA Streptococcus pneumoniae D39

67.173

94.54

0.635

  recA Lactococcus lactis subsp. cremoris KW2

65.569

95.977

0.629

  recA Streptococcus pyogenes NZ131

66.972

93.966

0.629

  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

65.758

94.828

0.624

  recA Latilactobacillus sakei subsp. sakei 23K

64.955

95.115

0.618

  recA Neisseria gonorrhoeae MS11

66.987

89.655

0.601

  recA Neisseria gonorrhoeae MS11

66.987

89.655

0.601

  recA Neisseria gonorrhoeae strain FA1090

66.987

89.655

0.601

  recA Acinetobacter baylyi ADP1

64.396

92.816

0.598

  recA Pseudomonas stutzeri DSM 10701

63.889

93.103

0.595

  recA Helicobacter pylori strain NCTC11637

61.976

95.977

0.595

  recA Helicobacter pylori 26695

61.976

95.977

0.595

  recA Acinetobacter baumannii D1279779

63.777

92.816

0.592

  recA Vibrio cholerae O1 biovar El Tor strain E7946

62.048

95.402

0.592

  recA Vibrio cholerae strain A1552

62.048

95.402

0.592

  recA Glaesserella parasuis strain SC1401

64.174

92.241

0.592

  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

63.272

93.103

0.589

  recA Ralstonia pseudosolanacearum GMI1000

66.558

88.506

0.589

  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

60.681

92.816

0.563