Detailed information    

insolico Bioinformatically predicted

Overview


Name   recA   Type   Machinery gene
Locus tag   WIS29_RS12080 Genome accession   NZ_CP149673
Coordinates   2696064..2697110 (-) Length   348 a.a.
NCBI ID   WP_003428218.1    Uniprot ID   A0A031WFI0
Organism   Clostridioides difficile strain Z1322PCD0060     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2648270..2725482 2696064..2697110 within 0


Gene organization within MGE regions


Location: 2648270..2725482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WIS29_RS11860 (WIS29_11850) - 2651242..2652225 (-) 984 WP_003438452.1 phage tail protein -
  WIS29_RS11865 (WIS29_11855) - 2652226..2652924 (-) 699 WP_003438451.1 putative phage tail protein -
  WIS29_RS11870 (WIS29_11860) - 2652921..2653973 (-) 1053 WP_003438450.1 baseplate J/gp47 family protein -
  WIS29_RS11875 (WIS29_11865) - 2653966..2654394 (-) 429 WP_003438449.1 DUF2634 domain-containing protein -
  WIS29_RS11880 (WIS29_11870) - 2654394..2654720 (-) 327 WP_003438447.1 DUF2577 domain-containing protein -
  WIS29_RS11885 (WIS29_11875) - 2654736..2656265 (-) 1530 WP_003438440.1 NlpC/P60 family protein -
  WIS29_RS11890 (WIS29_11880) - 2656547..2656969 (-) 423 WP_003428168.1 phage baseplate protein -
  WIS29_RS11895 (WIS29_11885) - 2656969..2659422 (-) 2454 WP_003438437.1 hypothetical protein -
  WIS29_RS11900 (WIS29_11890) - 2659422..2659589 (-) 168 WP_003419648.1 hypothetical protein -
  WIS29_RS11905 (WIS29_11895) - 2659616..2660062 (-) 447 WP_003419647.1 phage portal protein -
  WIS29_RS11910 (WIS29_11900) - 2660176..2660604 (-) 429 WP_003419646.1 phage tail tube protein -
  WIS29_RS11915 (WIS29_11905) - 2660618..2661682 (-) 1065 WP_003438414.1 phage tail sheath subtilisin-like domain-containing protein -
  WIS29_RS11920 (WIS29_11910) - 2661687..2662130 (-) 444 WP_003438413.1 DUF6838 family protein -
  WIS29_RS11925 (WIS29_11915) - 2662379..2662819 (-) 441 WP_003428183.1 hypothetical protein -
  WIS29_RS11930 (WIS29_11920) - 2662924..2663121 (-) 198 WP_003419641.1 helix-turn-helix transcriptional regulator -
  WIS29_RS11935 (WIS29_11925) - 2663290..2663610 (+) 321 WP_003428185.1 XRE family transcriptional regulator -
  WIS29_RS11940 (WIS29_11930) - 2663692..2664198 (+) 507 WP_003428186.1 hypothetical protein -
  WIS29_RS11945 (WIS29_11935) - 2664899..2665654 (+) 756 WP_009889131.1 peptidylprolyl isomerase -
  WIS29_RS11950 (WIS29_11940) - 2665797..2666225 (-) 429 WP_009889129.1 CBS domain-containing protein -
  WIS29_RS11955 (WIS29_11945) - 2666646..2666846 (-) 201 WP_003419624.1 cold-shock protein -
  WIS29_RS11960 (WIS29_11950) - 2667029..2668183 (-) 1155 WP_009902426.1 amidase domain-containing protein -
  WIS29_RS11965 (WIS29_11955) - 2668434..2669255 (+) 822 WP_003438396.1 pyridoxamine kinase -
  WIS29_RS11970 (WIS29_11960) - 2669331..2670719 (-) 1389 WP_021368953.1 two-component system sensor histidine kinase -
  WIS29_RS11975 (WIS29_11965) - 2670854..2671612 (-) 759 WP_021363441.1 lantibiotic immunity ABC transporter MutG family permease subunit -
  WIS29_RS11980 (WIS29_11970) - 2671614..2672330 (-) 717 WP_003438387.1 lantibiotic immunity ABC transporter MutE/EpiE family permease subunit -
  WIS29_RS11985 (WIS29_11975) - 2672332..2673039 (-) 708 WP_016729592.1 lantibiotic protection ABC transporter ATP-binding protein -
  WIS29_RS11990 (WIS29_11980) - 2673155..2673691 (-) 537 WP_003428196.1 lipoprotein -
  WIS29_RS11995 (WIS29_11985) - 2673793..2674863 (-) 1071 WP_003438380.1 DUF4097 family beta strand repeat-containing protein -
  WIS29_RS12000 (WIS29_11990) - 2674856..2675914 (-) 1059 WP_003438377.1 DUF1700 domain-containing protein -
  WIS29_RS12005 (WIS29_11995) - 2675901..2676233 (-) 333 WP_009889116.1 PadR family transcriptional regulator -
  WIS29_RS12010 (WIS29_12000) - 2676432..2677553 (+) 1122 WP_003438374.1 glycosyltransferase -
  WIS29_RS12015 (WIS29_12005) - 2677681..2679579 (-) 1899 WP_003428202.1 glutamine synthetase -
  WIS29_RS12020 (WIS29_12010) - 2679894..2680670 (+) 777 WP_003438370.1 3'-5' exonuclease -
  WIS29_RS12025 (WIS29_12015) - 2680762..2681514 (-) 753 WP_003438367.1 exodeoxyribonuclease III -
  WIS29_RS12030 (WIS29_12020) galT 2681675..2682625 (-) 951 WP_003438360.1 galactose-1-phosphate uridylyltransferase -
  WIS29_RS12035 (WIS29_12025) - 2683010..2684206 (+) 1197 WP_003438357.1 pyridoxal phosphate-dependent aminotransferase -
  WIS29_RS12040 (WIS29_12030) - 2684541..2684993 (-) 453 WP_003419561.1 MarR family transcriptional regulator -
  WIS29_RS12045 (WIS29_12035) purB 2685304..2686749 (-) 1446 WP_003438353.1 adenylosuccinate lyase -
  WIS29_RS12050 (WIS29_12040) - 2686893..2687801 (-) 909 WP_009889110.1 tyrosine-type recombinase/integrase -
  WIS29_RS12055 (WIS29_12045) - 2688058..2688882 (+) 825 WP_003438350.1 undecaprenyl-diphosphate phosphatase -
  WIS29_RS12060 (WIS29_12050) lepB 2688922..2689452 (+) 531 WP_009902411.1 signal peptidase I -
  WIS29_RS12065 (WIS29_12055) - 2689534..2691315 (-) 1782 WP_003438344.1 UvrD-helicase domain-containing protein -
  WIS29_RS12070 (WIS29_12060) - 2691697..2694033 (-) 2337 WP_003438341.1 diguanylate cyclase -
  WIS29_RS12075 (WIS29_12065) rny 2694347..2695888 (-) 1542 WP_003428216.1 ribonuclease Y -
  WIS29_RS12080 (WIS29_12070) recA 2696064..2697110 (-) 1047 WP_003428218.1 recombinase RecA Machinery gene
  WIS29_RS12085 (WIS29_12075) pgsA 2697398..2697940 (-) 543 WP_021370268.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  WIS29_RS12090 (WIS29_12080) rimO 2697927..2699261 (-) 1335 WP_003438333.1 30S ribosomal protein S12 methylthiotransferase RimO -
  WIS29_RS12095 (WIS29_12085) mnmH 2699255..2700313 (-) 1059 WP_003438330.1 tRNA 2-selenouridine(34) synthase MnmH -
  WIS29_RS12100 (WIS29_12090) - 2700316..2702727 (-) 2412 WP_003438328.1 DNA translocase FtsK -
  WIS29_RS12105 (WIS29_12095) - 2702850..2703584 (-) 735 WP_003428224.1 ATP-dependent Clp protease proteolytic subunit -
  WIS29_RS12110 (WIS29_12100) dapG 2703697..2704920 (-) 1224 WP_003438324.1 aspartate kinase -
  WIS29_RS12115 (WIS29_12105) - 2704930..2705175 (-) 246 WP_003419503.1 YlmC/YmxH family sporulation protein -
  WIS29_RS12120 (WIS29_12110) - 2705210..2706457 (-) 1248 WP_003438321.1 pitrilysin family protein -
  WIS29_RS12125 (WIS29_12115) - 2706615..2707358 (-) 744 WP_003438319.1 polysaccharide deacetylase family protein -
  WIS29_RS12130 (WIS29_12120) pnp 2707624..2709735 (-) 2112 WP_003438316.1 polyribonucleotide nucleotidyltransferase -
  WIS29_RS12135 (WIS29_12125) - 2709860..2710705 (-) 846 WP_009889099.1 DegV family protein -
  WIS29_RS12140 (WIS29_12130) rpsO 2710945..2711202 (-) 258 WP_003419491.1 30S ribosomal protein S15 -
  WIS29_RS12145 (WIS29_12135) - 2711306..2712235 (-) 930 WP_003438310.1 bifunctional riboflavin kinase/FAD synthetase -
  WIS29_RS12150 (WIS29_12140) truB 2712366..2713271 (-) 906 WP_003428232.1 tRNA pseudouridine(55) synthase TruB -
  WIS29_RS12155 (WIS29_12145) - 2713284..2714267 (-) 984 WP_003438308.1 bifunctional oligoribonuclease/PAP phosphatase NrnA -
  WIS29_RS12160 (WIS29_12150) rbfA 2714248..2714628 (-) 381 WP_003428234.1 30S ribosome-binding factor RbfA -
  WIS29_RS12165 (WIS29_12155) infB 2714671..2716611 (-) 1941 WP_003438306.1 translation initiation factor IF-2 -
  WIS29_RS12170 (WIS29_12160) - 2716633..2716944 (-) 312 WP_003419470.1 ribosomal L7Ae/L30e/S12e/Gadd45 family protein -
  WIS29_RS12175 (WIS29_12165) - 2716934..2717215 (-) 282 WP_003419469.1 YlxR family protein -
  WIS29_RS12180 (WIS29_12170) nusA 2717222..2718376 (-) 1155 WP_003428238.1 transcription termination factor NusA -
  WIS29_RS12185 (WIS29_12175) rimP 2718406..2718867 (-) 462 WP_003438303.1 ribosome maturation factor RimP -
  WIS29_RS12190 (WIS29_12180) - 2719071..2723369 (-) 4299 WP_003438301.1 PolC-type DNA polymerase III -
  WIS29_RS12195 (WIS29_12185) acd 2723584..2725407 (-) 1824 WP_003438294.1 N-acetylglucosaminidase -

Sequence


Protein


Download         Length: 348 a.a.        Molecular weight: 37377.68 Da        Isoelectric Point: 4.8236

>NTDB_id=960670 WIS29_RS12080 WP_003428218.1 2696064..2697110(-) (recA) [Clostridioides difficile strain Z1322PCD0060]
MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTGAIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSC
VASAQKDGGIAAFIDAEHALDPVYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKFYSSVRLDVRKIDTIKQGDKVIG
SRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLLDIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEI
DEKVRAFYNLNEEHEESGTSVSKEIVEE

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=960670 WIS29_RS12080 WP_003428218.1 2696064..2697110(-) (recA) [Clostridioides difficile strain Z1322PCD0060]
ATGAGTGTAGATCAAGAAAAATTAAAAGCGTTGAATGAAGCTTTAGGTAAAATTGAAAAAGATTTTGGTAAAGGTTCAGT
AATGAAATTGGGAGAAGCAACATCTATGTCTATAGATGTTATATCAACAGGAGCGATTGGTTTAGACATAGCTATTGGTA
TAGGAGGTCTACCTAGAGGGAGAATAGTTGAAGTATATGGTCCAGAATCTTCTGGTAAGACTACTGTTGCGCTTAGTTGT
GTAGCATCAGCTCAAAAAGATGGAGGAATAGCTGCATTTATAGATGCAGAACATGCACTTGACCCAGTATATGCAAAAGC
TTTGGGTGTGGATGTTGATAACCTAATAATATCTCAACCAGATACAGGTGAACAGGCTTTAGAGATAGCAGAGGCATTGA
TAAGAAGTGGAGCGATAGATATAATAGTAATAGACTCAGTAGCAGCATTAGTTCCAAAGGCTGAAATAGATGGAGATATG
GGTGATTCTCATGTAGGTTTACAAGCTAGACTTATGTCACAAGCACTTAGAAAGTTAACTGGTTCAATTAAAAAATCAAA
TTGTGTTGCTATATTTATAAACCAGTTAAGAGAGAAAGTAGGAATAATGTTTGGAAACCCAGAAACTACTACTGGAGGAC
GTGCACTAAAATTCTATTCATCAGTTAGATTGGATGTTAGAAAAATAGATACAATAAAACAAGGTGATAAAGTTATAGGT
AGTAGAACTAGAGTTAAAGTTGTTAAAAACAAAGTAGCACCACCATTTAAGCAAGCTGAATTTGATATAATGTATGGAGA
AGGGATTTCAAAAATTGGAGACCTTTTAGATATAGCTGCTGATGTAGATATAGTTAAAAAATCAGGTTCATGGTATAGTT
ACAATGATACTAAACTTGGACAAGGAAGAGAAAATGTTAAAAAATTCTTGGAAGATAATTTAGATTTAACTACTGAAATA
GATGAGAAAGTTAGAGCATTTTACAATTTAAATGAAGAACATGAAGAATCAGGTACTTCAGTATCAAAAGAAATTGTAGA
AGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A031WFI0

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Streptococcus mutans UA159

66.762

100

0.67

  recA Streptococcus mitis SK321

67.456

97.126

0.655

  recA Streptococcus pneumoniae Rx1

65.507

99.138

0.649

  recA Streptococcus pneumoniae R6

65.507

99.138

0.649

  recA Streptococcus pneumoniae D39

65.507

99.138

0.649

  recA Streptococcus pneumoniae TIGR4

65.507

99.138

0.649

  recA Streptococcus mitis NCTC 12261

68.085

94.54

0.644

  recA Bacillus subtilis subsp. subtilis str. 168

69.565

92.529

0.644

  recA Lactococcus lactis subsp. cremoris KW2

65.569

95.977

0.629

  recA Streptococcus pyogenes NZ131

66.972

93.966

0.629

  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

65.758

94.828

0.624

  recA Latilactobacillus sakei subsp. sakei 23K

64.955

95.115

0.618

  recA Neisseria gonorrhoeae MS11

66.049

93.103

0.615

  recA Neisseria gonorrhoeae MS11

66.049

93.103

0.615

  recA Neisseria gonorrhoeae strain FA1090

66.049

93.103

0.615

  recA Acinetobacter baylyi ADP1

64.396

92.816

0.598

  recA Pseudomonas stutzeri DSM 10701

63.889

93.103

0.595

  recA Helicobacter pylori 26695

61.976

95.977

0.595

  recA Helicobacter pylori strain NCTC11637

61.976

95.977

0.595

  recA Acinetobacter baumannii D1279779

63.777

92.816

0.592

  recA Vibrio cholerae O1 biovar El Tor strain E7946

62.048

95.402

0.592

  recA Vibrio cholerae strain A1552

62.048

95.402

0.592

  recA Glaesserella parasuis strain SC1401

64.174

92.241

0.592

  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

63.272

93.103

0.589

  recA Ralstonia pseudosolanacearum GMI1000

66.558

88.506

0.589

  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

60.681

92.816

0.563