Detailed information NTG0905970

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ABLU29_RS05150
• Genome accession: NZ_CP157498
• Coordinates: 914985..917324 (+)
• Length: 779 a.a.
• NCBI ID: WP_085624108.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain 2B-5
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 909985..922324

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABLU29_RS05130 (ABLU29_05120)	-	911009..911722 (+)	714	WP_003131466.1	glucosamine-6-phosphate deaminase	-
ABLU29_RS05135 (ABLU29_05125)	rny	911953..913548 (+)	1596	WP_003131467.1	ribonuclease Y	-
ABLU29_RS05140 (ABLU29_05130)	gmk	913696..914313 (+)	618	WP_003131468.1	guanylate kinase	-
ABLU29_RS05145 (ABLU29_05135)	rpoZ	914493..914846 (+)	354	WP_010906189.1	DNA-directed RNA polymerase subunit omega	-
ABLU29_RS05150 (ABLU29_05140)	priA	914985..917324 (+)	2340	WP_085624108.1	primosomal protein N'	Machinery gene
ABLU29_RS05155 (ABLU29_05145)	-	917383..917757 (+)	375	WP_228248357.1	nuclear transport factor 2 family protein	-
ABLU29_RS05160 (ABLU29_05150)	-	917770..918291 (+)	522	WP_260317749.1	NUDIX hydrolase	-
ABLU29_RS05165 (ABLU29_05155)	-	918257..918721 (+)	465	WP_012898447.1	VOC family protein	-
ABLU29_RS05170 (ABLU29_05160)	fmt	918718..919677 (+)	960	WP_033899823.1	methionyl-tRNA formyltransferase	-
ABLU29_RS05175 (ABLU29_05165)	-	919852..920094 (+)	243	WP_003131476.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
ABLU29_RS05180 (ABLU29_05170)	amaP	920122..920682 (+)	561	WP_010906186.1	alkaline shock response membrane anchor protein AmaP	-
ABLU29_RS05185 (ABLU29_05175)	-	920693..920881 (+)	189	WP_012898445.1	DUF2273 domain-containing protein	-
ABLU29_RS05190 (ABLU29_05180)	-	920902..921408 (+)	507	WP_012898444.1	Asp23/Gls24 family envelope stress response protein	-

Sequence

Protein

• Length: 779 a.a.
• Molecular weight: 88918.19 Da
• Isoelectric Point: 6.5580

>NTDB_id=905970 ABLU29_RS05150 WP_085624108.1 914985..917324(+) (priA) [Lactococcus lactis strain 2B-5]
MKNAKVIVDVPLMQTDKPFSYSLSAEFENLLEVGMRVHVPFGSGNRLVQGIVIGFEDEELEPSVNLKGISELLDVEPVLT
DEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKSTDGTSVKWSQLTEAEKISSAKAVRLGQLSVEYLAKSKENK
KTEKFLSIPDIEKLAEFEIEKRAKKRLELKYFLLRLQEEKIAVKSLNFSSQIIKFFVEHDLVKIEEVEISRTKEKFEDVE
NDSAKILNSEQNSAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLKLGKTAIMLVPEISLTPQITNRFIARFGDKVA
IMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFAPVENLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGARLVL
GSATPSLETRARAMRGVYELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLTKIKEKIDKDEQVILMLNRRGYS
SFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVEGIPQTCPNCHSRKIRYYGSGTQKVEEELREIMPEARVLRMD
VDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRADKK
GEVLIQTFNPHHYAIEMTKAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEIAQLLKQNLTEKAKIL
GPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRVLIDSEPQNFV

Nucleotide

• Length: 2340 bp

>NTDB_id=905970 ABLU29_RS05150 WP_085624108.1 914985..917324(+) (priA) [Lactococcus lactis strain 2B-5]
ATGAAAAATGCTAAAGTCATCGTTGATGTGCCTCTGATGCAGACGGATAAACCCTTTTCTTACAGCCTTTCAGCTGAATT
TGAAAATTTGCTTGAAGTGGGCATGCGGGTCCATGTTCCTTTTGGAAGTGGAAACCGGCTTGTTCAAGGAATTGTCATTG
GATTTGAAGATGAAGAACTTGAGCCGTCAGTAAATTTGAAAGGAATTTCTGAACTACTCGACGTTGAGCCAGTCCTAACG
GATGAACAGCTTTGGTTGGCAGATGAAATGCGTCATTCAGTATTTTCTTATAAAATTTCATGTCTAAAAGCCATGTTACC
TAATCTTTTGAATTCTAATTATGACAAGCTCCTAAAATCGACTGACGGAACTTCTGTCAAATGGTCACAACTGACAGAAG
CAGAAAAAATCTCGTCAGCAAAGGCTGTGCGTTTGGGTCAGCTGTCAGTAGAATATTTAGCAAAATCTAAAGAAAATAAA
AAAACAGAAAAATTCTTATCTATTCCAGATATCGAAAAACTAGCTGAATTTGAAATTGAAAAGAGAGCTAAAAAACGCTT
AGAGTTGAAATATTTTCTATTAAGACTTCAAGAAGAAAAAATTGCTGTCAAATCATTAAATTTTTCTTCACAAATCATTA
AATTTTTCGTTGAGCATGACCTAGTCAAAATTGAAGAGGTTGAAATTTCGAGGACGAAAGAAAAATTTGAAGATGTGGAA
AATGACAGTGCTAAAATTCTCAACTCTGAACAAAATTCAGCTTACGAAAAAATAATTGCATCTTCTGACAAACCATTTTT
GTTGGAAGGAATTACTGGTTCAGGTAAAACAGAAGTTTACTTACAGACCATTGACCATTTTCTCAAACTCGGTAAAACAG
CAATTATGCTTGTGCCAGAAATTTCTTTGACGCCACAGATTACTAATCGATTTATTGCTCGTTTTGGAGATAAAGTGGCG
ATTATGCATTCTGGTTTATCTGATGGTGAAAAATATGATGAATGGCGAAAAATTAAATCTGGCAAAGCTCAGGTAGTTGT
CGGTGCACGTTCAGCTATTTTTGCACCTGTTGAAAATTTGGGAATTATCATCATTGATGAAGAACATGAAACTTCATATA
AGCAAGATTCAAGTCCCCGTTATCATGCAAGAGATATTGCGCTTTTGCGTGCAAAATGGCATGGTGCAAGACTTGTTTTA
GGTTCTGCAACGCCAAGTTTAGAAACGCGAGCACGTGCCATGCGCGGAGTTTATGAACTTTTATTACTGACAAAAAGGGC
AAATGAAGCAGCAATCCTGCCAGAAATAAAAATCATTGATATGCGCAGTGAAATTTCTGACCAATCTGCTAATTTTTCAC
AATCTTTACTGACAAAAATAAAAGAAAAAATTGACAAAGATGAACAAGTCATTTTGATGTTGAATCGAAGGGGTTATTCT
TCATTTGTCATGTGTCGTGAATGTGGTTTTGTTGTTGATTGTCCCAATTGCGATATTTCCTTGACCCTTCACATGGATAC
CCGAACTTTGAATTGTCATTATTGTGGTCATGTCGAAGGAATTCCGCAAACTTGTCCTAATTGTCATTCACGAAAAATTC
GTTATTACGGTTCAGGAACGCAAAAAGTTGAAGAAGAACTTCGAGAAATCATGCCTGAAGCACGAGTTTTGCGAATGGAT
GTAGATACGACTCGGACAAAAAATGCTCACGAAAAAATTCTTGAAAAGTTTGGTAATCATGAAGCTGATATCCTCTTAGG
AACTCAGATGATTGCCAAAGGTTTAGATTTTCCAAACGTAACCTTAGTGGGTGTCATTAACGCAGATACTGGCCTAAATC
TACCTGATTTTAGAGCGAGTGAGCGTTCCTTTGAATTGTTGATGCAGGTGGCTGGACGAGCTGGACGAGCAGATAAAAAA
GGTGAAGTATTAATTCAAACTTTCAATCCACATCATTATGCTATTGAAATGACCAAGGCTCAAGATTACGAAAAATTTTA
TGAAATGGAAATGAGATTTCGTCGTGAACTTTCCTATCCACCTTATTATTTCACCGTTCAAGTGCTTGTTTCTCATCAAG
ATGAAGAAGTTGCCATTCAAAAATCTTATGAGATCGCTCAATTACTCAAGCAAAATTTGACAGAAAAAGCTAAAATATTA
GGGCCAACCGCCAGACCAATTGCCAGAACACATAACCTTTATCATTATCAAATCTTAGTTAAATATCGTTTTGAAGAAGA
ATTAGAATCAGCTTTAAATCAAGTGCTTGAATTAACCCAACTTCGGGATAACAAAAATTTACGAGTATTGATTGACAGTG
AGCCACAAAATTTTGTATAA

Domains

Predicted by InterProScan.

• Type III restriction enzyme, res subunit (ResIII) (247-405)

• Primosomal protein N C-terminal domain (PriA_C) (681-775)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-107)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (494-518)

• Helicase conserved C-terminal domain (Helicase_C) (561-637)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	47.512	100	0.49