Detailed information    

insolico Bioinformatically predicted

Overview


Name   recA   Type   Machinery gene
Locus tag   CDIFJ21_RS06475 Genome accession   NZ_CP134690
Coordinates   1388650..1389696 (+) Length   348 a.a.
NCBI ID   WP_003428218.1    Uniprot ID   A0A031WFI0
Organism   Clostridioides difficile strain J2_1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1360279..1437479 1388650..1389696 within 0


Gene organization within MGE regions


Location: 1360279..1437479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDIFJ21_RS06360 (CDIFJ21_12680) acd 1360354..1362177 (+) 1824 WP_021373393.1 N-acetylglucosaminidase -
  CDIFJ21_RS06365 (CDIFJ21_12690) - 1362392..1366690 (+) 4299 WP_003438301.1 PolC-type DNA polymerase III -
  CDIFJ21_RS06370 (CDIFJ21_12700) rimP 1366894..1367355 (+) 462 WP_003438303.1 ribosome maturation factor RimP -
  CDIFJ21_RS06375 (CDIFJ21_12710) nusA 1367385..1368539 (+) 1155 WP_003428238.1 transcription termination factor NusA -
  CDIFJ21_RS06380 (CDIFJ21_12720) rnpM 1368546..1368827 (+) 282 WP_003419469.1 RNase P modulator RnpM -
  CDIFJ21_RS06385 (CDIFJ21_12730) - 1368817..1369128 (+) 312 WP_003419470.1 L7Ae/L30e/S12e/Gadd45 family ribosomal protein -
  CDIFJ21_RS06390 (CDIFJ21_12740) infB 1369150..1371090 (+) 1941 WP_003438306.1 translation initiation factor IF-2 -
  CDIFJ21_RS06395 (CDIFJ21_12750) rbfA 1371133..1371513 (+) 381 WP_003428234.1 30S ribosome-binding factor RbfA -
  CDIFJ21_RS06400 (CDIFJ21_12760) - 1371494..1372477 (+) 984 WP_003438308.1 DHH family phosphoesterase -
  CDIFJ21_RS06405 (CDIFJ21_12770) truB 1372490..1373395 (+) 906 WP_003428232.1 tRNA pseudouridine(55) synthase TruB -
  CDIFJ21_RS06410 (CDIFJ21_12780) - 1373526..1374455 (+) 930 WP_003438310.1 bifunctional riboflavin kinase/FAD synthetase -
  CDIFJ21_RS06415 (CDIFJ21_12790) rpsO 1374559..1374816 (+) 258 WP_003419491.1 30S ribosomal protein S15 -
  CDIFJ21_RS06420 (CDIFJ21_12800) - 1375056..1375901 (+) 846 WP_009889099.1 DegV family protein -
  CDIFJ21_RS06425 (CDIFJ21_12810) pnp 1376026..1378137 (+) 2112 WP_003438316.1 polyribonucleotide nucleotidyltransferase -
  CDIFJ21_RS06430 (CDIFJ21_12820) - 1378403..1379146 (+) 744 WP_021373395.1 polysaccharide deacetylase family protein -
  CDIFJ21_RS06435 (CDIFJ21_12830) - 1379304..1380551 (+) 1248 WP_021373396.1 M16 family metallopeptidase -
  CDIFJ21_RS06440 (CDIFJ21_12840) - 1380586..1380831 (+) 246 WP_003419503.1 YlmC/YmxH family sporulation protein -
  CDIFJ21_RS06445 (CDIFJ21_12850) dapG 1380841..1382064 (+) 1224 WP_003438324.1 aspartate kinase -
  CDIFJ21_RS06450 (CDIFJ21_12860) - 1382177..1382911 (+) 735 WP_003428224.1 ClpP family protease -
  CDIFJ21_RS06455 (CDIFJ21_12870) - 1383034..1385445 (+) 2412 WP_003438328.1 FtsK/SpoIIIE family DNA translocase -
  CDIFJ21_RS06460 (CDIFJ21_12880) mnmH 1385448..1386506 (+) 1059 WP_003438330.1 tRNA 2-selenouridine(34) synthase MnmH -
  CDIFJ21_RS06465 (CDIFJ21_12890) rimO 1386500..1387834 (+) 1335 WP_003438333.1 30S ribosomal protein S12 methylthiotransferase RimO -
  CDIFJ21_RS06470 (CDIFJ21_12900) pgsA 1387821..1388363 (+) 543 WP_021370268.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  CDIFJ21_RS06475 (CDIFJ21_12910) recA 1388650..1389696 (+) 1047 WP_003428218.1 recombinase RecA Machinery gene
  CDIFJ21_RS06480 (CDIFJ21_12920) rny 1389872..1391413 (+) 1542 WP_003428216.1 ribonuclease Y -
  CDIFJ21_RS06485 (CDIFJ21_12930) - 1391727..1393217 (+) 1491 WP_321570628.1 cache domain-containing protein -
  CDIFJ21_RS19195 (CDIFJ21_12940) - 1393266..1394063 (+) 798 WP_416795310.1 diguanylate cyclase -
  CDIFJ21_RS06500 (CDIFJ21_12950) - 1394445..1396226 (+) 1782 WP_003438344.1 UvrD-helicase domain-containing protein -
  CDIFJ21_RS06505 (CDIFJ21_12960) lepB 1396308..1396838 (-) 531 WP_009902411.1 signal peptidase I -
  CDIFJ21_RS06510 (CDIFJ21_12970) - 1396878..1397702 (-) 825 WP_003438350.1 undecaprenyl-diphosphate phosphatase -
  CDIFJ21_RS06515 (CDIFJ21_12980) - 1397959..1398867 (+) 909 WP_009889110.1 tyrosine-type recombinase/integrase -
  CDIFJ21_RS06520 (CDIFJ21_12990) purB 1399011..1400456 (+) 1446 WP_003438353.1 adenylosuccinate lyase -
  CDIFJ21_RS06525 (CDIFJ21_13000) - 1400767..1401219 (+) 453 WP_003419561.1 MarR family winged helix-turn-helix transcriptional regulator -
  CDIFJ21_RS06530 (CDIFJ21_13010) - 1401554..1402750 (-) 1197 WP_003438357.1 pyridoxal phosphate-dependent aminotransferase -
  CDIFJ21_RS06535 (CDIFJ21_13020) galT 1403135..1404085 (+) 951 WP_003438360.1 galactose-1-phosphate uridylyltransferase -
  CDIFJ21_RS06540 (CDIFJ21_13030) - 1404246..1404998 (+) 753 WP_003438367.1 exodeoxyribonuclease III -
  CDIFJ21_RS06545 (CDIFJ21_13040) - 1405090..1405866 (-) 777 WP_003438370.1 3'-5' exonuclease -
  CDIFJ21_RS06550 (CDIFJ21_13050) - 1406181..1408079 (+) 1899 WP_003428202.1 glutamine synthetase -
  CDIFJ21_RS06555 (CDIFJ21_13060) - 1408207..1409328 (-) 1122 WP_003438374.1 MGDG synthase family glycosyltransferase -
  CDIFJ21_RS06560 (CDIFJ21_13070) - 1409527..1409859 (+) 333 WP_009889116.1 PadR family transcriptional regulator -
  CDIFJ21_RS06565 (CDIFJ21_13080) - 1409846..1410904 (+) 1059 WP_003438377.1 DUF1700 domain-containing protein -
  CDIFJ21_RS06570 (CDIFJ21_13090) - 1410897..1411967 (+) 1071 WP_003438380.1 DUF4097 family beta strand repeat-containing protein -
  CDIFJ21_RS06575 (CDIFJ21_13100) - 1412069..1412605 (+) 537 WP_003428196.1 lipoprotein -
  CDIFJ21_RS06580 (CDIFJ21_13110) - 1412721..1413428 (+) 708 WP_016729592.1 lantibiotic protection ABC transporter ATP-binding protein -
  CDIFJ21_RS06585 (CDIFJ21_13120) - 1413430..1414146 (+) 717 WP_003438387.1 lantibiotic immunity ABC transporter MutE/EpiE family permease subunit -
  CDIFJ21_RS06590 (CDIFJ21_13130) - 1414148..1414906 (+) 759 WP_021363441.1 lantibiotic immunity ABC transporter MutG family permease subunit -
  CDIFJ21_RS06595 (CDIFJ21_13140) - 1415090..1416427 (+) 1338 WP_235665142.1 two-component system sensor histidine kinase -
  CDIFJ21_RS06600 (CDIFJ21_13150) - 1416503..1417324 (-) 822 WP_003438396.1 pyridoxamine kinase -
  CDIFJ21_RS06605 (CDIFJ21_13160) - 1417575..1418729 (+) 1155 WP_009902426.1 amidase domain-containing protein -
  CDIFJ21_RS06610 (CDIFJ21_13170) - 1418912..1419112 (+) 201 WP_003419624.1 cold-shock protein -
  CDIFJ21_RS06615 (CDIFJ21_13180) - 1419533..1419961 (+) 429 WP_009889129.1 CBS domain-containing protein -
  CDIFJ21_RS06620 (CDIFJ21_13190) - 1420104..1420859 (-) 756 WP_009889131.1 peptidylprolyl isomerase -
  CDIFJ21_RS06625 (CDIFJ21_13210) - 1421560..1422066 (-) 507 WP_003428186.1 ImmA/IrrE family metallo-endopeptidase -
  CDIFJ21_RS06630 (CDIFJ21_13220) - 1422148..1422468 (-) 321 WP_003428185.1 XRE family transcriptional regulator -
  CDIFJ21_RS06635 (CDIFJ21_13230) - 1422637..1422834 (+) 198 WP_003419641.1 helix-turn-helix transcriptional regulator -
  CDIFJ21_RS06640 (CDIFJ21_13240) - 1422939..1423379 (+) 441 WP_003428183.1 hypothetical protein -
  CDIFJ21_RS06645 (CDIFJ21_13250) - 1423628..1424071 (+) 444 WP_021373399.1 phage tail terminator family protein -
  CDIFJ21_RS06650 (CDIFJ21_13260) - 1424076..1425140 (+) 1065 WP_011861188.1 phage tail sheath subtilisin-like domain-containing protein -
  CDIFJ21_RS06655 (CDIFJ21_13270) - 1425154..1425582 (+) 429 WP_021373400.1 phage tail tube protein -
  CDIFJ21_RS06660 (CDIFJ21_13280) - 1425708..1426154 (+) 447 WP_003419647.1 phage tail assembly chaperone -
  CDIFJ21_RS06665 (CDIFJ21_13290) - 1426181..1426348 (+) 168 WP_003419648.1 hypothetical protein -
  CDIFJ21_RS06670 (CDIFJ21_13300) - 1426348..1428801 (+) 2454 WP_021373401.1 hypothetical protein -
  CDIFJ21_RS06675 (CDIFJ21_13310) - 1428801..1429223 (+) 423 WP_003428168.1 phage baseplate protein -
  CDIFJ21_RS06680 (CDIFJ21_13320) - 1429505..1431034 (+) 1530 WP_009902435.1 NlpC/P60 family protein -
  CDIFJ21_RS06685 (CDIFJ21_13330) - 1431050..1431376 (+) 327 WP_003438447.1 DUF2577 domain-containing protein -
  CDIFJ21_RS06690 (CDIFJ21_13340) - 1431376..1431804 (+) 429 WP_003428161.1 DUF2634 domain-containing protein -
  CDIFJ21_RS06695 (CDIFJ21_13350) - 1431797..1432828 (+) 1032 WP_231309988.1 baseplate J/gp47 family protein -
  CDIFJ21_RS06700 (CDIFJ21_13360) - 1432825..1433523 (+) 699 WP_003428156.1 putative phage tail protein -
  CDIFJ21_RS06705 (CDIFJ21_13370) - 1433524..1434507 (+) 984 WP_021366456.1 phage tail protein -

Sequence


Protein


Download         Length: 348 a.a.        Molecular weight: 37377.68 Da        Isoelectric Point: 4.8236

>NTDB_id=882842 CDIFJ21_RS06475 WP_003428218.1 1388650..1389696(+) (recA) [Clostridioides difficile strain J2_1]
MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTGAIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSC
VASAQKDGGIAAFIDAEHALDPVYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKFYSSVRLDVRKIDTIKQGDKVIG
SRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLLDIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEI
DEKVRAFYNLNEEHEESGTSVSKEIVEE

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=882842 CDIFJ21_RS06475 WP_003428218.1 1388650..1389696(+) (recA) [Clostridioides difficile strain J2_1]
ATGAGTGTAGATCAAGAAAAATTAAAAGCGTTGAATGAAGCTTTAGGTAAAATTGAAAAAGATTTTGGTAAAGGTTCAGT
AATGAAATTGGGAGAAGCAACATCTATGTCTATAGATGTTATATCAACAGGAGCGATTGGTTTAGACATAGCTATTGGTA
TAGGAGGTCTACCTAGAGGGAGAATAGTTGAAGTATATGGTCCAGAATCTTCTGGTAAGACTACTGTTGCGCTTAGTTGT
GTAGCATCAGCTCAAAAAGATGGAGGAATAGCTGCATTTATAGATGCAGAACATGCACTTGACCCAGTATATGCAAAAGC
TTTGGGTGTGGATGTTGATAACCTAATAATATCTCAACCAGATACAGGTGAACAGGCTTTAGAGATAGCAGAGGCATTGA
TAAGAAGTGGAGCGATAGATATAATAGTAATAGACTCAGTAGCAGCATTAGTTCCAAAGGCTGAAATAGATGGAGATATG
GGTGATTCTCATGTAGGTTTACAAGCTAGACTTATGTCACAAGCACTTAGAAAGTTAACTGGTTCAATTAAAAAATCAAA
TTGTGTTGCTATATTTATAAACCAGTTAAGAGAGAAAGTAGGAATAATGTTTGGAAACCCAGAAACTACTACTGGAGGAC
GTGCACTAAAATTCTATTCATCAGTTAGATTGGATGTTAGAAAAATAGATACAATAAAACAAGGTGATAAAGTTATAGGT
AGTAGAACTAGAGTTAAAGTTGTTAAAAACAAAGTAGCACCACCATTTAAGCAAGCTGAATTTGATATAATGTATGGAGA
AGGGATTTCAAAAATTGGAGACCTTTTAGATATAGCTGCTGATGTAGATATAGTTAAAAAATCAGGTTCATGGTATAGTT
ACAATGATACTAAACTTGGACAAGGAAGAGAAAATGTTAAAAAATTCTTGGAAGATAATTTAGATTTAACTACTGAAATA
GATGAGAAAGTTAGAGCATTTTACAATTTAAATGAAGAACATGAAGAATCAGGTACTTCAGTATCAAAAGAAATTGTAGA
AGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A031WFI0

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Streptococcus mutans UA159

66.762

100

0.67

  recA Streptococcus mitis SK321

67.456

97.126

0.655

  recA Streptococcus pneumoniae Rx1

65.507

99.138

0.649

  recA Streptococcus pneumoniae R6

65.507

99.138

0.649

  recA Streptococcus pneumoniae D39

65.507

99.138

0.649

  recA Streptococcus pneumoniae TIGR4

65.507

99.138

0.649

  recA Streptococcus mitis NCTC 12261

68.085

94.54

0.644

  recA Bacillus subtilis subsp. subtilis str. 168

69.565

92.529

0.644

  recA Lactococcus lactis subsp. cremoris KW2

65.569

95.977

0.629

  recA Streptococcus pyogenes NZ131

66.972

93.966

0.629

  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

65.758

94.828

0.624

  recA Latilactobacillus sakei subsp. sakei 23K

64.955

95.115

0.618

  recA Neisseria gonorrhoeae MS11

66.049

93.103

0.615

  recA Neisseria gonorrhoeae MS11

66.049

93.103

0.615

  recA Neisseria gonorrhoeae strain FA1090

66.049

93.103

0.615

  recA Acinetobacter baylyi ADP1

64.396

92.816

0.598

  recA Pseudomonas stutzeri DSM 10701

63.889

93.103

0.595

  recA Helicobacter pylori 26695

61.976

95.977

0.595

  recA Helicobacter pylori strain NCTC11637

61.976

95.977

0.595

  recA Acinetobacter baumannii D1279779

63.777

92.816

0.592

  recA Vibrio cholerae O1 biovar El Tor strain E7946

62.048

95.402

0.592

  recA Vibrio cholerae strain A1552

62.048

95.402

0.592

  recA Glaesserella parasuis strain SC1401

64.174

92.241

0.592

  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

63.272

93.103

0.589

  recA Ralstonia pseudosolanacearum GMI1000

66.558

88.506

0.589

  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

60.681

92.816

0.563