Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q5417_RS09585 Genome accession   NZ_CP130750
Coordinates   2023462..2024970 (+) Length   502 a.a.
NCBI ID   WP_079759847.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain AU10241     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2018462..2029970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5417_RS09565 (Q5417_09565) - 2018494..2019703 (+) 1210 Protein_1892 O-succinylhomoserine sulfhydrylase -
  Q5417_RS09570 (Q5417_09570) - 2019700..2020467 (+) 768 WP_003103539.1 SDR family oxidoreductase -
  Q5417_RS09575 (Q5417_09575) xcpQ 2020554..2022530 (-) 1977 WP_003103536.1 GspD family T2SS secretin variant XcpQ -
  Q5417_RS09580 (Q5417_09580) xcpP 2022535..2023242 (-) 708 WP_003119750.1 type II secretion system protein N -
  Q5417_RS09585 (Q5417_09585) pilB 2023462..2024970 (+) 1509 WP_079759847.1 GspE family T2SS ATPase variant XcpR Machinery gene
  Q5417_RS09590 (Q5417_09590) xcpS 2024970..2026187 (+) 1218 WP_003091376.1 GspF family T2SS innner membrane protein variant XcpS -
  Q5417_RS09595 (Q5417_09595) xcpT 2026192..2026638 (+) 447 WP_003119751.1 GspG family T2SS major pseudopilin variant XcpT -
  Q5417_RS09600 (Q5417_09600) xcpU 2026645..2027163 (+) 519 WP_003103530.1 GspH family T2SS minor pseudopilin variant XcpU -
  Q5417_RS09605 (Q5417_09605) xcpV 2027160..2027549 (+) 390 WP_003103528.1 GspI family T2SS minor pseudopilin variant XcpV -
  Q5417_RS09610 (Q5417_09610) xcpW 2027546..2028259 (+) 714 WP_003103526.1 GspJ family T2SS minor pseudopilin variant XcpW -
  Q5417_RS09615 (Q5417_09615) xcpX 2028256..2029257 (+) 1002 WP_003103524.1 GspK family T2SS minor pseudopilin variant XcpX -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55612.84 Da        Isoelectric Point: 6.2074

>NTDB_id=787624 Q5417_RS09585 WP_079759847.1 2023462..2024970(+) (pilB) [Pseudomonas aeruginosa strain AU10241]
MMTAPLPDIAAPAAPPRRLPFSFAKRQGLLFLCLEEQYWLACRPQVELAAIAEAQRFAGRRLPLRALGEDAFNQALAASY
QHDSSAAMQLAEDLGGSLDLAALADQVPETEDLMEQEDDAPIIRLINAILGEAIRENASDIHLETFEKRLVVRFRVDGVL
REVLEPKRELAALLVSRIKVMARLDIAEKRIPQDGRISLRVGGREVDIRVSTLPSANGERVVLRLLDKQAGRLNLQHLGM
SERDRKLMDETVRKPHGILLVTGPTGSGKTTTLYASLTTLNDRTRNILTVEDPIEYHLEGIGQTQVNAKVDMTFARGLRA
ILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTNSAIGAITRLVDMGIEPFLLFSSMLGVLAQRLVRVLCPACK
EPYRADEAECALLGVDPAAPPTLHRARGCGECHQHGYRGRTGIYELVVFDDHMRSLIHNESSEQEMTRHARTSGPSIRDD
GRRKVLEGVTTVEEVLRVTREE

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=787624 Q5417_RS09585 WP_079759847.1 2023462..2024970(+) (pilB) [Pseudomonas aeruginosa strain AU10241]
ATGATGACAGCCCCATTACCCGACATCGCCGCGCCGGCCGCACCGCCACGGCGTCTGCCGTTCAGCTTCGCCAAGCGCCA
GGGCCTGTTGTTCCTGTGCCTGGAGGAGCAGTACTGGCTGGCCTGCCGGCCGCAGGTGGAGCTGGCGGCGATCGCCGAGG
CGCAGCGCTTCGCCGGCCGCCGCCTGCCGCTCAGGGCGCTCGGCGAGGACGCCTTCAACCAGGCCCTGGCGGCCTCCTAC
CAGCACGACTCCTCGGCGGCCATGCAGCTCGCCGAGGACCTCGGCGGCAGCCTGGACCTCGCCGCGCTGGCCGACCAGGT
CCCGGAAACCGAGGACCTGATGGAGCAGGAGGACGACGCGCCGATCATTCGCCTGATCAACGCGATCCTCGGCGAGGCGA
TCCGCGAGAACGCCTCGGACATCCACCTGGAAACCTTCGAGAAGCGCCTGGTGGTGCGCTTCCGCGTCGACGGCGTGCTG
CGCGAGGTGCTGGAGCCCAAGCGCGAGCTGGCGGCCCTGCTGGTATCGCGGATCAAGGTCATGGCGCGGCTGGACATCGC
CGAGAAGCGTATTCCCCAGGATGGGCGGATTTCCCTGCGGGTCGGCGGGCGCGAGGTGGACATCCGCGTCTCGACCCTGC
CCTCGGCCAACGGTGAACGGGTGGTGCTGCGTCTGCTCGACAAGCAGGCCGGGCGCCTGAACCTGCAGCACCTGGGCATG
AGCGAGCGCGACCGCAAGCTGATGGACGAAACGGTGCGCAAGCCGCACGGCATCCTGCTGGTCACCGGCCCCACCGGCTC
GGGCAAGACCACCACCCTGTACGCCAGCCTGACCACGCTCAACGACCGCACGCGGAACATCCTCACCGTCGAGGATCCGA
TCGAATACCACCTGGAGGGCATCGGCCAGACCCAGGTCAACGCCAAGGTCGACATGACCTTCGCCCGCGGCCTGCGGGCG
ATCCTCCGGCAGGACCCGGACGTGGTGATGGTCGGCGAGATCCGCGACCGGGAGACCGCCGAGATCGCGGTGCAGGCTTC
GCTGACCGGCCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCATCGGTGCGATCACCCGCCTGGTGGACATGGGCA
TCGAGCCGTTCCTGCTGTTCTCCTCCATGCTTGGCGTGCTGGCCCAGCGCCTGGTGCGGGTGCTCTGCCCGGCGTGCAAG
GAGCCCTACCGGGCCGACGAGGCCGAGTGCGCGCTGCTCGGCGTCGATCCCGCCGCGCCGCCGACCCTGCATCGCGCCCG
CGGCTGCGGCGAGTGCCACCAGCATGGCTATCGCGGGCGTACCGGTATCTATGAGCTGGTGGTGTTCGACGACCACATGC
GCTCGCTGATCCACAACGAATCCTCCGAGCAGGAAATGACCCGGCATGCCCGCACCAGCGGACCGAGCATCCGCGACGAC
GGTCGGCGCAAGGTGCTGGAAGGCGTCACCACGGTCGAGGAAGTCCTGCGCGTGACCCGGGAAGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.737

97.012

0.424
  pilB Vibrio campbellii strain DS40M4

43.949

93.825

0.412
  pilB/pilB1 Synechocystis sp. PCC 6803

42.041

97.61

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

47.045

84.263

0.396
  pilB Acinetobacter baumannii D1279779

47.88

79.88

0.382
  pilF Thermus thermophilus HB27

42.921

88.645

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.187

76.892

0.371
  pilF Neisseria gonorrhoeae MS11

46.954

78.486

0.369
  pilB Acinetobacter baylyi ADP1

47.68

77.291

0.369
  pilB Legionella pneumophila strain ERS1305867

47.423

77.291

0.367