Detailed information    

insolico Bioinformatically predicted

Overview

Name   recA   Type   Machinery gene
Locus tag   POF62_RS10335 Genome accession   NZ_CP116707
Coordinates   2024130..2025269 (-) Length   379 a.a.
NCBI ID   WP_001085741.1    Uniprot ID   A0AAV3JP53
Organism   Streptococcus agalactiae strain A909 sCas9revertant     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2019130..2030269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF62_RS10305 (POF62_10305) - 2020494..2022035 (-) 1542 WP_000230040.1 membrane protein -
  POF62_RS10310 (POF62_10310) - 2022081..2022203 (-) 123 WP_001285708.1 hypothetical protein -
  POF62_RS10315 (POF62_10315) - 2022286..2022603 (-) 318 WP_000940931.1 DUF1292 domain-containing protein -
  POF62_RS10320 (POF62_10320) ruvX 2022629..2023048 (-) 420 WP_001222109.1 Holliday junction resolvase RuvX -
  POF62_RS10325 (POF62_10325) - 2023048..2023314 (-) 267 WP_000507056.1 IreB family regulatory phosphoprotein -
  POF62_RS10330 (POF62_10330) spx 2023516..2023914 (-) 399 WP_000591161.1 transcriptional regulator Spx -
  POF62_RS10335 (POF62_10335) recA 2024130..2025269 (-) 1140 WP_001085741.1 recombinase RecA Machinery gene
  POF62_RS10340 (POF62_10340) cinA 2025343..2026602 (-) 1260 WP_001200975.1 competence/damage-inducible protein A Machinery gene
  POF62_RS10345 (POF62_10345) - 2026691..2027242 (-) 552 WP_000817341.1 DNA-3-methyladenine glycosylase I -
  POF62_RS10350 (POF62_10350) ruvA 2027265..2027855 (-) 591 WP_000272490.1 Holliday junction branch migration protein RuvA -
  POF62_RS10355 (POF62_10355) - 2027857..2029089 (-) 1233 WP_000657472.1 MFS transporter -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 40917.44 Da        Isoelectric Point: 5.0474

>NTDB_id=779285 POF62_RS10335 WP_001085741.1 2024130..2025269(-) (recA) [Streptococcus agalactiae strain A909 sCas9revertant]
MAKKTKKAEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGERAEQKVQVMSSGSLALDIALGAGGYPKGRIVEIYGP
ESSGKTTVALHAVAQAQKEGGIAAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGNPETTPGGRALKFYSSVRLDVRG
NTQIKGTGEHKDHNVGKETKIKVVKNKVAPPFREAFVEIMYGEGISRTGELIKIASDLDIIQKAGAWYSYNGEKIGQGSE
NAKKYLADNPAIFDEIDHKVRVHFGMTEDDSPVQSELVEEKNEADDLVLDLDNAIEIEE

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=779285 POF62_RS10335 WP_001085741.1 2024130..2025269(-) (recA) [Streptococcus agalactiae strain A909 sCas9revertant]
TTGGCTAAAAAAACGAAAAAAGCAGAAGAAATTACTAAGAAATTCGGTGATGAACGTCGAAAAGCCTTAGATGATGCTTT
GAAAAATATTGAAAAAGATTTTGGTAAGGGCGCAGTGATGCGCTTAGGTGAGCGAGCTGAACAAAAGGTTCAAGTAATGA
GTTCAGGTTCTCTTGCTTTAGATATCGCTTTAGGAGCAGGTGGATATCCTAAAGGACGTATCGTCGAAATTTATGGACCA
GAGTCATCTGGTAAAACAACGGTTGCCCTTCATGCCGTAGCTCAAGCCCAAAAGGAAGGTGGGATTGCTGCCTTTATTGA
TGCTGAGCACGCTCTTGACCCAGCCTATGCTGCGGCTCTTGGAGTTAATATTGATGAGTTACTCTTGTCTCAACCTGACT
CAGGTGAGCAAGGTCTTGAAATTGCAGGTAAATTGATTGATTCAGGCGCAGTTGATTTAGTTGTTGTCGATTCAGTTGCA
GCCCTTGTTCCTCGTGCTGAGATTGATGGTGATATTGGTGACAGCCACGTTGGTTTGCAAGCTCGTATGATGAGTCAAGC
AATGCGTAAATTGTCAGCATCCATTAACAAGACAAAGACAATCGCAATTTTTATCAACCAGCTAAGGGAAAAAGTAGGCG
TTATGTTTGGTAACCCTGAAACAACACCTGGTGGTCGTGCTTTGAAATTTTATTCATCGGTTCGTCTAGATGTTCGTGGT
AATACTCAAATCAAGGGGACTGGTGAGCATAAAGATCATAATGTTGGTAAAGAGACAAAAATCAAAGTTGTTAAAAATAA
AGTTGCTCCACCATTCCGTGAAGCTTTTGTCGAAATTATGTATGGTGAAGGTATTTCTCGCACGGGTGAGTTAATTAAAA
TTGCTAGTGATTTGGATATTATCCAAAAAGCAGGTGCATGGTACTCATATAATGGTGAGAAAATTGGACAAGGTTCTGAA
AATGCCAAAAAATATTTAGCAGATAATCCAGCAATTTTTGATGAAATTGACCATAAAGTGCGCGTGCACTTTGGAATGAC
TGAAGATGATTCGCCAGTTCAGTCTGAGCTCGTTGAAGAAAAAAATGAAGCTGATGATCTTGTTTTAGATTTGGACAATG
CTATTGAAATTGAGGAATAA

Domains

Predicted by InterproScan.

recA bacterial DNA recombination protein (RecA)

(21-286)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Streptococcus mutans UA159

89.034

100

0.9
  recA Streptococcus pyogenes NZ131

89.182

100

0.892
  recA Streptococcus mitis NCTC 12261

85.564

100

0.86
  recA Streptococcus mitis SK321

85.039

100

0.855
  recA Streptococcus pneumoniae Rx1

83.636

100

0.85
  recA Streptococcus pneumoniae D39

83.636

100

0.85
  recA Streptococcus pneumoniae R6

83.636

100

0.85
  recA Streptococcus pneumoniae TIGR4

83.636

100

0.85
  recA Lactococcus lactis subsp. cremoris KW2

76.724

91.821

0.704
  recA Latilactobacillus sakei subsp. sakei 23K

69.118

89.71

0.62
  recA Bacillus subtilis subsp. subtilis str. 168

68.997

86.807

0.599
  recA Acinetobacter baylyi ADP1

59.659

92.876

0.554
  recA Neisseria gonorrhoeae MS11

63.142

87.335

0.551
  recA Neisseria gonorrhoeae MS11

63.142

87.335

0.551
  recA Neisseria gonorrhoeae strain FA1090

63.142

87.335

0.551
  recA Vibrio cholerae strain A1552

59.429

92.348

0.549
  recA Vibrio cholerae O1 biovar El Tor strain E7946

59.429

92.348

0.549
  recA Acinetobacter baumannii D1279779

59.091

92.876

0.549
  recA Glaesserella parasuis strain SC1401

60.294

89.71

0.541
  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

61.398

86.807

0.533
  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

62.229

85.224

0.53
  recA Pseudomonas stutzeri DSM 10701

57.514

91.293

0.525
  recA Ralstonia pseudosolanacearum GMI1000

59.819

87.335

0.522
  recA Helicobacter pylori strain NCTC11637

56.812

91.029

0.517
  recA Helicobacter pylori 26695

56.812

91.029

0.517
  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

57.478

89.974

0.517