Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   PBU98_RS00505 Genome accession   NZ_CP115821
Coordinates   113155..113823 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain E1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 108155..118823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PBU98_RS00475 yicC 108174..109037 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  PBU98_RS00480 rph 109164..109880 (+) 717 WP_001247093.1 ribonuclease PH -
  PBU98_RS00485 pyrE 109946..110587 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  PBU98_RS00490 slmA 110624..111220 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  PBU98_RS00495 dut 111327..111785 (-) 459 WP_000976070.1 dUTP diphosphatase -
  PBU98_RS00500 coaBC 111763..112983 (-) 1221 WP_000050171.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  PBU98_RS00505 radC 113155..113823 (+) 669 WP_001297375.1 RadC family protein Machinery gene
  PBU98_RS00510 rpmB 114040..114276 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  PBU98_RS00515 rpmG 114297..114464 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  PBU98_RS00520 mutM 114562..115371 (+) 810 WP_001114542.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  PBU98_RS00525 coaD 115410..115889 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  PBU98_RS00530 waaA 115897..117174 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  PBU98_RS00535 rfaQ 117587..118645 (+) 1059 WP_001521608.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=706004 PBU98_RS00505 WP_001297375.1 113155..113823(+) (radC) [Escherichia coli strain E1]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=706004 PBU98_RS00505 WP_001297375.1 113155..113823(+) (radC) [Escherichia coli strain E1]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTAGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGGGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAATGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496