Detailed information    

insolico Bioinformatically predicted

Overview


Name   recA   Type   Machinery gene
Locus tag   PST29_RS06395 Genome accession   NZ_AP014628
Coordinates   1423065..1424117 (+) Length   350 a.a.
NCBI ID   WP_011059579.1    Uniprot ID   A0A2C9EHI1
Organism   Pseudomonas sp. St29     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1365212..1424117 1423065..1424117 within 0


Gene organization within MGE regions


Location: 1365212..1424117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PST29_RS06095 (PST29_1221) - 1365212..1366111 (-) 900 WP_060837774.1 LysR substrate-binding domain-containing protein -
  PST29_RS06100 (PST29_1222) - 1366216..1367328 (+) 1113 WP_060837775.1 S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase -
  PST29_RS06105 (PST29_1223) fghA 1367416..1368261 (+) 846 WP_060842606.1 S-formylglutathione hydrolase -
  PST29_RS06110 (PST29_1224) ispF 1368314..1368787 (+) 474 WP_060837777.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  PST29_RS06115 (PST29_1225) truD 1368784..1369842 (+) 1059 WP_060842607.1 tRNA pseudouridine(13) synthase TruD -
  PST29_RS06120 (PST29_1226) surE 1369830..1370579 (+) 750 WP_047302063.1 5'/3'-nucleotidase SurE -
  PST29_RS06125 (PST29_1227) - 1370621..1371256 (+) 636 WP_173655928.1 protein-L-isoaspartate(D-aspartate) O-methyltransferase -
  PST29_RS30730 (PST29_1228) - 1371468..1372340 (+) 873 WP_082729181.1 peptidoglycan DD-metalloendopeptidase family protein -
  PST29_RS06130 (PST29_1229) rpoS 1372447..1373454 (+) 1008 WP_016964675.1 RNA polymerase sigma factor RpoS -
  PST29_RS06135 (PST29_1230) fdxA 1373972..1374295 (-) 324 WP_016964674.1 ferredoxin FdxA -
  PST29_RS06140 (PST29_1231) mutS 1374438..1377017 (-) 2580 WP_060842608.1 DNA mismatch repair protein MutS -
  PST29_RS06145 (PST29_1232) - 1377204..1377950 (+) 747 WP_060837780.1 XRE family transcriptional regulator -
  PST29_RS06150 (PST29_1233) - 1378469..1378819 (+) 351 WP_016967167.1 phage holin family protein -
  PST29_RS06155 (PST29_1234) - 1378900..1379421 (+) 522 WP_060842609.1 hypothetical protein -
  PST29_RS06160 (PST29_1235) - 1379418..1380008 (+) 591 WP_060842610.1 phage baseplate assembly protein V -
  PST29_RS06165 (PST29_1236) - 1380089..1380292 (+) 204 WP_060837783.1 hypothetical protein -
  PST29_RS06170 (PST29_1237) - 1380297..1380623 (+) 327 WP_060842611.1 GPW/gp25 family protein -
  PST29_RS06175 (PST29_1238) - 1380620..1381501 (+) 882 WP_060842612.1 baseplate J/gp47 family protein -
  PST29_RS06180 (PST29_1239) - 1381503..1382108 (+) 606 WP_060842613.1 phage tail protein I -
  PST29_RS31560 (PST29_1240) - 1382105..1384525 (+) 2421 WP_148659596.1 phage tail protein -
  PST29_RS30735 (PST29_1241) - 1384536..1384961 (+) 426 WP_232513205.1 phage tail assembly chaperone -
  PST29_RS06195 (PST29_1242) - 1385064..1386230 (+) 1167 WP_060842615.1 phage tail sheath family protein -
  PST29_RS06200 (PST29_1243) - 1386243..1386752 (+) 510 WP_016965333.1 phage major tail tube protein -
  PST29_RS06205 (PST29_1244) - 1386765..1387070 (+) 306 WP_060842616.1 phage tail assembly protein -
  PST29_RS06210 (PST29_1245) - 1387212..1389767 (+) 2556 WP_060842617.1 phage tail tape measure protein -
  PST29_RS06215 (PST29_1246) - 1389777..1390622 (+) 846 WP_060842618.1 phage tail protein -
  PST29_RS06220 (PST29_1247) - 1390597..1390803 (+) 207 WP_060842619.1 tail protein X -
  PST29_RS06225 (PST29_1248) - 1390865..1391920 (+) 1056 WP_060842620.1 contractile injection system protein, VgrG/Pvc8 family -
  PST29_RS06230 (PST29_1249) - 1392025..1393176 (+) 1152 WP_060842621.1 glycosyl hydrolase family 18 protein -
  PST29_RS06235 (PST29_1250) - 1393243..1393434 (+) 192 WP_019093183.1 hypothetical protein -
  PST29_RS06240 (PST29_1251) - 1393525..1394016 (+) 492 WP_016964892.1 phage tail tube protein -
  PST29_RS06245 (PST29_1252) - 1394025..1394375 (+) 351 WP_016964891.1 phage tail assembly chaperone family protein, TAC -
  PST29_RS06250 (PST29_1253) - 1394405..1394659 (+) 255 WP_060845073.1 hypothetical protein -
  PST29_RS06255 (PST29_1254) - 1394719..1396128 (+) 1410 WP_060842622.1 phage tail tape measure protein -
  PST29_RS06260 (PST29_1255) - 1396168..1396506 (+) 339 WP_060842623.1 phage tail protein -
  PST29_RS32310 (PST29_1256) - 1396503..1397747 (+) 1245 WP_177342978.1 hypothetical protein -
  PST29_RS30745 (PST29_1257) - 1397748..1398044 (+) 297 WP_082729182.1 hypothetical protein -
  PST29_RS06265 (PST29_1258) - 1398044..1398307 (+) 264 WP_060842624.1 hypothetical protein -
  PST29_RS06270 (PST29_1259) - 1398356..1399039 (+) 684 WP_019093175.1 phage minor tail protein L -
  PST29_RS06275 (PST29_1260) - 1399042..1399818 (+) 777 WP_060842625.1 Mov34/MPN/PAD-1 family protein -
  PST29_RS06280 (PST29_1261) - 1399874..1400506 (+) 633 WP_060842626.1 tail assembly protein -
  PST29_RS06285 (PST29_1262) - 1400573..1401181 (+) 609 WP_060842627.1 tail assembly protein -
  PST29_RS06290 (PST29_1263) - 1401242..1404334 (+) 3093 WP_060842628.1 host specificity protein J -
  PST29_RS31565 (PST29_1264) - 1404343..1404624 (+) 282 WP_148659597.1 hypothetical protein -
  PST29_RS31570 (PST29_1265) - 1404621..1405340 (+) 720 WP_148659598.1 hypothetical protein -
  PST29_RS06295 (PST29_1266) - 1405760..1406344 (+) 585 WP_060842629.1 hypothetical protein -
  PST29_RS06300 (PST29_1267) - 1406341..1406523 (+) 183 WP_047302105.1 DUF2635 domain-containing protein -
  PST29_RS06305 (PST29_1268) - 1406523..1408019 (+) 1497 WP_060842630.1 phage tail sheath subtilisin-like domain-containing protein -
  PST29_RS06310 (PST29_1269) - 1408086..1408433 (+) 348 WP_016965085.1 phage tail tube protein -
  PST29_RS06315 (PST29_1270) - 1408430..1408726 (+) 297 WP_060842631.1 phage tail assembly protein -
  PST29_RS06320 (PST29_1271) - 1408857..1410578 (+) 1722 WP_060842632.1 phage tail protein -
  PST29_RS06325 (PST29_1272) - 1410568..1411812 (+) 1245 WP_060842633.1 DNA circularization N-terminal domain-containing protein -
  PST29_RS06330 (PST29_1273) - 1411816..1412856 (+) 1041 WP_060842634.1 baseplate protein -
  PST29_RS06335 (PST29_1274) - 1412965..1413471 (+) 507 WP_060837800.1 phage baseplate assembly protein -
  PST29_RS06340 (PST29_1275) - 1413474..1413872 (+) 399 WP_060837801.1 phage GP46 family protein -
  PST29_RS06345 (PST29_1276) - 1413862..1414902 (+) 1041 WP_060842635.1 baseplate J/gp47 family protein -
  PST29_RS06350 (PST29_1277) - 1414890..1415489 (+) 600 WP_060842636.1 putative phage tail protein -
  PST29_RS06355 (PST29_1278) - 1415505..1417292 (+) 1788 WP_060842637.1 phage tail protein -
  PST29_RS30750 (PST29_1279) - 1417289..1417651 (+) 363 WP_082729183.1 tail fiber assembly protein -
  PST29_RS06360 (PST29_1280) - 1417750..1418979 (+) 1230 WP_232513206.1 phage tail protein -
  PST29_RS30755 (PST29_1281) - 1418992..1419420 (+) 429 WP_082729184.1 tail fiber assembly protein -
  PST29_RS06365 (PST29_1282) - 1419471..1420034 (+) 564 WP_016966077.1 glycoside hydrolase family 19 protein -
  PST29_RS06370 (PST29_1283) - 1420191..1420697 (+) 507 WP_060842638.1 hypothetical protein -
  PST29_RS30760 - 1420768..1420953 (+) 186 WP_082729320.1 Com family DNA-binding transcriptional regulator -
  PST29_RS06375 (PST29_1284) - 1420962..1421804 (+) 843 WP_060837808.1 putidacin L1 family lectin-like bacteriocin -
  PST29_RS06385 (PST29_1285) - 1421982..1422314 (+) 333 WP_020372545.1 hypothetical protein -
  PST29_RS06390 (PST29_1286) - 1422481..1422981 (+) 501 WP_060842639.1 nicotinamide-nucleotide amidohydrolase family protein -
  PST29_RS06395 (PST29_1287) recA 1423065..1424117 (+) 1053 WP_011059579.1 recombinase RecA Machinery gene

Sequence


Protein


Download         Length: 350 a.a.        Molecular weight: 37462.95 Da        Isoelectric Point: 5.5108

>NTDB_id=66327 PST29_RS06395 WP_011059579.1 1423065..1424117(+) (recA) [Pseudomonas sp. St29]
MDDNKKKALAAALGQIERQFGKGAVMRMGDHDRQAIPAISTGSLGLDIALGIGGLPKGRIVEIYGPESSGKTTLTLSVIA
QAQKAGATCAFVDAEHALDPEYAGKLGVNVDDLLVSQPDTGEQALEITDMLVRSNAVDVIIVDSVAALVPKAEIEGEMGD
MHVGLQARLMSQALRKITGNIKNANCLVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRTGAVKEGDEVVGSE
TRVKIVKNKVAPPFRQAEFQILYGKGIYLNGEIIDLGVLHGFLEKSGAWYSYQGNKIGQGKANSAKFLQDNPEIGNALEK
QIREKLLTASPDVKANPVKETEDDMADADI

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=66327 PST29_RS06395 WP_011059579.1 1423065..1424117(+) (recA) [Pseudomonas sp. St29]
ATGGACGACAACAAGAAGAAAGCCTTGGCTGCGGCCCTGGGTCAGATCGAACGTCAATTCGGCAAGGGTGCCGTAATGCG
TATGGGCGATCATGACCGTCAGGCGATCCCGGCCATTTCCACCGGCTCTCTGGGTCTGGACATCGCTCTGGGCATTGGCG
GTCTGCCAAAAGGCCGTATCGTTGAAATCTACGGTCCTGAATCGTCCGGTAAGACCACCCTGACCCTGTCGGTCATCGCT
CAGGCGCAGAAAGCCGGTGCCACTTGTGCCTTCGTCGATGCCGAGCACGCGCTGGACCCGGAATACGCCGGCAAGCTGGG
GGTCAATGTCGACGATCTGCTGGTTTCTCAGCCGGATACCGGCGAGCAGGCCCTGGAAATCACCGACATGCTGGTGCGTT
CCAACGCGGTTGACGTGATCATCGTCGACTCCGTGGCGGCTCTGGTGCCCAAGGCTGAAATCGAAGGCGAAATGGGCGAC
ATGCACGTGGGCCTGCAAGCCCGCCTGATGTCCCAGGCGCTGCGTAAAATCACCGGTAACATCAAGAACGCCAACTGCCT
GGTGATCTTCATCAACCAGATCCGCATGAAGATCGGCGTGATGTTCGGCAGTCCGGAAACCACCACCGGTGGTAACGCCC
TGAAGTTCTACGCTTCGGTTCGTCTGGACATCCGTCGTACCGGCGCGGTGAAGGAAGGCGATGAGGTGGTCGGTAGCGAA
ACCCGAGTCAAGATCGTCAAGAACAAGGTGGCTCCGCCGTTCCGTCAGGCCGAGTTCCAGATTCTTTACGGCAAGGGTAT
CTACCTCAACGGCGAGATCATCGATCTTGGCGTGCTGCACGGTTTCCTGGAAAAATCCGGTGCCTGGTACAGCTACCAGG
GCAACAAGATCGGTCAGGGCAAGGCCAACTCGGCCAAGTTCCTGCAGGACAACCCGGAAATCGGCAACGCGTTGGAGAAG
CAGATTCGCGAGAAGCTGCTGACAGCCTCGCCAGACGTCAAAGCCAATCCGGTCAAAGAGACCGAAGACGATATGGCCGA
CGCTGATATCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A2C9EHI1

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Pseudomonas stutzeri DSM 10701

88.857

100

0.889

  recA Acinetobacter baylyi ADP1

73.926

99.714

0.737

  recA Acinetobacter baumannii D1279779

72.464

98.571

0.714

  recA Vibrio cholerae strain A1552

74.618

93.429

0.697

  recA Vibrio cholerae O1 biovar El Tor strain E7946

74.618

93.429

0.697

  recA Glaesserella parasuis strain SC1401

69.164

99.143

0.686

  recA Ralstonia pseudosolanacearum GMI1000

67.705

100

0.683

  recA Neisseria gonorrhoeae MS11

71.605

92.571

0.663

  recA Neisseria gonorrhoeae MS11

71.605

92.571

0.663

  recA Neisseria gonorrhoeae strain FA1090

71.605

92.571

0.663

  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

63.314

96.571

0.611

  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

62.575

95.429

0.597

  recA Helicobacter pylori strain NCTC11637

59.483

99.429

0.591

  recA Helicobacter pylori 26695

58.908

99.429

0.586

  recA Bacillus subtilis subsp. subtilis str. 168

61.682

91.714

0.566

  recA Latilactobacillus sakei subsp. sakei 23K

56.686

98.286

0.557

  recA Streptococcus mutans UA159

59.692

92.857

0.554

  recA Streptococcus pneumoniae D39

58.589

93.143

0.546

  recA Streptococcus pneumoniae Rx1

58.589

93.143

0.546

  recA Streptococcus pneumoniae R6

58.589

93.143

0.546

  recA Streptococcus pneumoniae TIGR4

58.589

93.143

0.546

  recA Streptococcus mitis NCTC 12261

58.824

92.286

0.543

  recA Streptococcus mitis SK321

58.514

92.286

0.54

  recA Streptococcus pyogenes NZ131

57.622

93.714

0.54

  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.442

93.143

0.526

  recA Lactococcus lactis subsp. cremoris KW2

56.037

92.286

0.517


Multiple sequence alignment