Detailed information    

insolico Bioinformatically predicted

Overview


Name   recA   Type   Machinery gene
Locus tag   LRO88_RS09595 Genome accession   NZ_CP089148
Coordinates   1819742..1820788 (+) Length   348 a.a.
NCBI ID   WP_003245789.1    Uniprot ID   A0A199WHQ8
Organism   Bacillus subtilis strain NIB353     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1807371..1874847 1819742..1820788 within 0


Gene organization within MGE regions


Location: 1807371..1874847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRO88_RS09540 spoIIIE 1807371..1809734 (+) 2364 WP_021479278.1 DNA translocase SpoIIIE -
  LRO88_RS09545 ymfC 1809878..1810603 (+) 726 WP_003245732.1 GntR family transcriptional regulator -
  LRO88_RS09550 bcbE 1810742..1811953 (+) 1212 WP_031600514.1 bacillibactin exporter BcbE -
  LRO88_RS09555 yfmF 1812133..1813413 (+) 1281 WP_032725709.1 EF-P 5-aminopentanol modification-associated protein YfmF -
  LRO88_RS09560 yfmH 1813410..1814696 (+) 1287 WP_003244823.1 EF-P 5-aminopentanol modification-associated protein YfmH -
  LRO88_RS09565 ymfI 1814751..1815479 (+) 729 WP_015715897.1 elongation factor P 5-aminopentanone reductase -
  LRO88_RS09570 ymfJ 1815560..1815817 (+) 258 WP_003245199.1 DUF3243 domain-containing protein -
  LRO88_RS09575 - 1815947..1816738 (+) 792 WP_003220998.1 DUF3388 domain-containing protein -
  LRO88_RS09580 rodZ 1816757..1817671 (+) 915 WP_003244699.1 cell shape determination protein RodZ -
  LRO88_RS09585 pgsA 1817721..1818302 (+) 582 WP_122894526.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  LRO88_RS09590 cinA 1818320..1819570 (+) 1251 WP_122894525.1 competence/damage-inducible protein A Machinery gene
  LRO88_RS09595 recA 1819742..1820788 (+) 1047 WP_003245789.1 recombinase RecA Machinery gene
  LRO88_RS09600 pbpX 1820956..1822131 (+) 1176 WP_122894524.1 serine hydrolase domain-containing protein -
  LRO88_RS09605 rny 1822407..1823969 (+) 1563 WP_003221010.1 ribonuclease Y -
  LRO88_RS09610 ymdB 1824038..1824832 (+) 795 WP_003245138.1 2',3'-cyclic-nucleotide 2'-phosphodiesterase -
  LRO88_RS09615 spoVS 1825032..1825292 (+) 261 WP_003154135.1 stage V sporulation protein SpoVS -
  LRO88_RS09620 tdh 1825558..1826601 (+) 1044 WP_122894523.1 L-threonine 3-dehydrogenase -
  LRO88_RS09625 kbl 1826614..1827792 (+) 1179 WP_122894522.1 glycine C-acetyltransferase -
  LRO88_RS09630 miaB 1827940..1829469 (+) 1530 WP_231834275.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  LRO88_RS09635 ricA 1829471..1829902 (+) 432 WP_003231834.1 regulatory iron-sulfur-containing complex subunit RicA -
  LRO88_RS09640 cotE 1830163..1830708 (+) 546 WP_003231833.1 outer spore coat protein CotE -
  LRO88_RS09645 mutS 1830841..1833417 (+) 2577 WP_003231832.1 DNA mismatch repair protein MutS -
  LRO88_RS09650 mutL 1833433..1835316 (+) 1884 WP_122894521.1 DNA mismatch repair endonuclease MutL -
  LRO88_RS09655 ymzD 1835716..1836171 (-) 456 WP_122894520.1 regulatory YrvL family protein -
  LRO88_RS09660 - 1836326..1836883 (-) 558 WP_017694906.1 hypothetical protein -
  LRO88_RS09665 pksA 1837004..1837618 (+) 615 WP_231834276.1 TetR/AcrR family transcriptional regulator -
  LRO88_RS09670 - 1837757..1838113 (-) 357 WP_003231786.1 hypothetical protein -
  LRO88_RS09675 ymaE 1838192..1839016 (-) 825 WP_153529745.1 MBL fold metallo-hydrolase -
  LRO88_RS09680 aprX 1839126..1840454 (-) 1329 WP_046381045.1 serine protease AprX -
  LRO88_RS09685 - 1840679..1840912 (+) 234 WP_014476869.1 hypothetical protein -
  LRO88_RS09690 pghC 1841192..1841899 (+) 708 WP_003245758.1 poly-gamma-glutamate hydrolase family protein -
  LRO88_RS09695 ymaD 1841969..1842421 (+) 453 WP_003231775.1 OsmC family protein -
  LRO88_RS09700 ebrB 1842435..1842788 (-) 354 WP_014476870.1 multidrug efflux SMR transporter subunit EbrB -
  LRO88_RS09705 ebrA 1842802..1843119 (-) 318 WP_015483253.1 multidrug efflux SMR transporter subunit EbrA -
  LRO88_RS09710 - 1843254..1843529 (-) 276 WP_003245252.1 hypothetical protein -
  LRO88_RS09715 ymaF 1843618..1844031 (+) 414 WP_003231769.1 YmaF family protein -
  LRO88_RS09720 miaA 1844131..1845075 (+) 945 WP_021479248.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  LRO88_RS09725 hfq 1845115..1845336 (+) 222 WP_003221097.1 RNA chaperone Hfq -
  LRO88_RS09730 ymzC 1845531..1845803 (+) 273 WP_014479841.1 YmzC family protein -
  LRO88_RS09735 - 1845885..1846115 (+) 231 WP_003245262.1 hypothetical protein -
  LRO88_RS09740 nrdI 1846357..1846749 (+) 393 WP_159376809.1 class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI -
  LRO88_RS09745 nrdE 1846709..1848811 (+) 2103 WP_014479842.1 class 1b ribonucleoside-diphosphate reductase subunit alpha -
  LRO88_RS09750 nrdF 1848829..1849818 (+) 990 WP_072174042.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
  LRO88_RS09755 - 1849868..1850488 (+) 621 WP_072174041.1 hypothetical protein -
  LRO88_RS09760 cwlC 1850552..1851319 (-) 768 WP_032725747.1 sporulation-specific N-acetylmuramoyl-L-alanine amidase -
  LRO88_RS09765 spoVK 1851947..1852915 (+) 969 WP_159376811.1 stage V sporulation protein K -
  LRO88_RS09770 hflX 1853048..1854310 (+) 1263 WP_032725748.1 GTPase HflX -
  LRO88_RS09775 efpB 1854328..1855593 (+) 1266 WP_029317829.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -
  LRO88_RS09780 glnR 1855703..1856110 (+) 408 WP_014476884.1 transcriptional repressor GlnR -
  LRO88_RS09785 glnA 1856169..1857503 (+) 1335 WP_015252037.1 type I glutamate--ammonia ligase -
  LRO88_RS09790 - 1857622..1857756 (-) 135 WP_231834277.1 hypothetical protein -
  LRO88_RS09795 - 1857794..1858240 (-) 447 WP_231834278.1 site-specific integrase -
  LRO88_RS09800 - 1858371..1859126 (+) 756 Protein_1881 DnaB-like helicase C-terminal domain-containing protein -
  LRO88_RS09805 - 1859438..1860235 (+) 798 WP_038828302.1 hypothetical protein -
  LRO88_RS09810 - 1860336..1860803 (+) 468 WP_038828303.1 ArpU family phage packaging/lysis transcriptional regulator -
  LRO88_RS09815 - 1860968..1861753 (+) 786 WP_072174040.1 FRG domain-containing protein -
  LRO88_RS09820 - 1862112..1862477 (+) 366 WP_038828305.1 HNH endonuclease -
  LRO88_RS09825 - 1862514..1863215 (+) 702 WP_038828306.1 agmatine deiminase family protein -
  LRO88_RS09830 - 1863181..1863540 (+) 360 WP_231834279.1 hypothetical protein -
  LRO88_RS09835 - 1863565..1863865 (+) 301 Protein_1888 T7SS effector LXG polymorphic toxin -
  LRO88_RS09840 - 1864572..1866389 (+) 1818 WP_231834280.1 NTTRR-F1 domain -
  LRO88_RS09845 - 1866494..1867165 (-) 672 WP_124048669.1 glycosyltransferase family protein -
  LRO88_RS09850 - 1867162..1867869 (-) 708 WP_032725786.1 class I SAM-dependent methyltransferase -
  LRO88_RS09855 - 1867856..1868962 (-) 1107 WP_077671699.1 glycosyltransferase -
  LRO88_RS09860 - 1868959..1870359 (-) 1401 WP_396129889.1 glycosyltransferase -
  LRO88_RS09865 ynaD 1871006..1871542 (+) 537 WP_015483262.1 GNAT family N-acetyltransferase -
  LRO88_RS09870 - 1871621..1872523 (+) 903 WP_159376813.1 CPBP family intramembrane glutamic endopeptidase -
  LRO88_RS09875 - 1872612..1872992 (+) 381 WP_029726895.1 UPF0715 family protein -
  LRO88_RS09880 - 1873331..1873495 (+) 165 WP_154213091.1 hypothetical protein -
  LRO88_RS09885 - 1873571..1873801 (+) 231 WP_032725791.1 helix-turn-helix domain-containing protein -
  LRO88_RS09890 ynaE 1873798..1874433 (+) 636 WP_032725792.1 DUF3885 domain-containing protein -

Sequence


Protein


Download         Length: 348 a.a.        Molecular weight: 38059.21 Da        Isoelectric Point: 4.7315

>NTDB_id=635299 LRO88_RS09595 WP_003245789.1 1819742..1820788(+) (recA) [Bacillus subtilis strain NIB353]
MSDRQAALDMALKQIEKQFGKGSIMKLGEKTDTRISTVPSGSLALDTALGIGGYPRGRIIEVYGPESSGKTTVALHAIAE
VQQQGGQAAFIDAEHALDPVYAQKLGVNIEELLLSQPDTGEQALEIAEALVRSGAVDIVVVDSVAALVPKAEIEGDMGDS
HVGLQARLMSQALRKLSGAINKSKTIAIFINQIREKVGVMFGNPETTPGGRALKFYSSVRLEVRRAEQLKQGNDVMGNKT
KIKVVKNKVAPPFRTAEVDIMYGEGISKEGEIIDLGTELDIVQKSGSWYSYEEERLGQGRENAKQFLKENKDIMLMIQEQ
IREHYGLDNNGVVQQQAEETQEELEFEE

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=635299 LRO88_RS09595 WP_003245789.1 1819742..1820788(+) (recA) [Bacillus subtilis strain NIB353]
ATGAGTGATCGTCAGGCAGCCTTAGATATGGCTCTTAAACAAATAGAAAAACAGTTCGGCAAAGGTTCCATTATGAAACT
GGGAGAAAAGACAGATACAAGAATTTCTACTGTCCCAAGCGGCTCTCTAGCTCTTGATACAGCACTGGGAATTGGCGGAT
ATCCTCGCGGACGGATTATTGAAGTATACGGTCCTGAAAGCTCAGGTAAAACAACTGTGGCGCTTCATGCGATTGCTGAA
GTTCAGCAGCAGGGCGGACAAGCCGCGTTTATCGATGCGGAGCATGCGTTAGATCCGGTATACGCGCAAAAGCTCGGTGT
TAACATAGAAGAGCTTTTACTGTCTCAGCCTGACACAGGCGAGCAGGCGCTTGAAATTGCGGAAGCATTGGTTCGAAGCG
GGGCAGTTGACATTGTCGTTGTCGACTCTGTAGCCGCTCTCGTTCCGAAAGCGGAAATTGAAGGCGACATGGGAGATTCG
CATGTCGGTTTACAAGCACGCTTAATGTCTCAAGCGCTTCGTAAGCTTTCAGGGGCCATTAACAAATCGAAGACAATCGC
GATTTTCATTAACCAAATTCGTGAAAAAGTCGGTGTTATGTTCGGGAACCCGGAAACAACTCCTGGCGGCCGTGCGTTGA
AATTCTATTCTTCCGTGCGTCTTGAAGTGCGCCGTGCTGAACAGCTGAAACAAGGCAACGACGTAATGGGGAACAAAACG
AAAATCAAAGTCGTGAAAAACAAAGTGGCTCCGCCGTTCCGTACAGCCGAGGTTGACATTATGTACGGAGAAGGCATTTC
AAAAGAAGGCGAAATCATTGATCTAGGAACTGAACTTGATATCGTGCAAAAAAGCGGTTCATGGTACTCTTATGAAGAAG
AGCGTCTTGGCCAAGGCCGTGAAAATGCAAAACAATTCCTGAAAGAAAATAAAGATATCATGCTGATGATCCAGGAGCAA
ATTCGCGAACATTACGGCTTGGATAATAACGGAGTAGTGCAGCAGCAAGCTGAAGAGACACAAGAAGAACTCGAATTTGA
AGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A199WHQ8

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Bacillus subtilis subsp. subtilis str. 168

100

100

1

  recA Latilactobacillus sakei subsp. sakei 23K

74.924

93.966

0.704

  recA Streptococcus pneumoniae D39

66.477

100

0.672

  recA Streptococcus pneumoniae R6

66.477

100

0.672

  recA Streptococcus pneumoniae TIGR4

66.477

100

0.672

  recA Streptococcus pneumoniae Rx1

66.477

100

0.672

  recA Streptococcus mutans UA159

66.092

100

0.661

  recA Streptococcus mitis SK321

65.994

99.713

0.658

  recA Streptococcus mitis NCTC 12261

68.788

94.828

0.652

  recA Streptococcus pyogenes NZ131

67.683

94.253

0.638

  recA Neisseria gonorrhoeae strain FA1090

63.584

99.425

0.632

  recA Neisseria gonorrhoeae MS11

63.584

99.425

0.632

  recA Neisseria gonorrhoeae MS11

63.584

99.425

0.632

  recA Lactococcus lactis subsp. cremoris KW2

65.758

94.828

0.624

  recA Helicobacter pylori 26695

63.081

98.851

0.624

  recA Helicobacter pylori strain NCTC11637

63.081

98.851

0.624

  recA Ralstonia pseudosolanacearum GMI1000

64.742

94.54

0.612

  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

61.988

98.276

0.609

  recA Glaesserella parasuis strain SC1401

60.58

99.138

0.601

  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

63.914

93.966

0.601

  recA Vibrio cholerae O1 biovar El Tor strain E7946

60.465

98.851

0.598

  recA Vibrio cholerae strain A1552

60.465

98.851

0.598

  recA Acinetobacter baumannii D1279779

61.243

97.126

0.595

  recA Pseudomonas stutzeri DSM 10701

62.813

91.954

0.578

  recA Acinetobacter baylyi ADP1

62.422

92.529

0.578

  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

60.681

92.816

0.563