Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5O92_RS18975 Genome accession   NZ_CP104721
Coordinates   3955619..3956059 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain ECO3183     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3950619..3961059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O92_RS18955 (N5O92_18955) aroP 3951617..3952990 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  N5O92_RS18960 (N5O92_18960) ampE 3953033..3953887 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  N5O92_RS18965 (N5O92_18965) ampD 3953884..3954435 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N5O92_RS18970 (N5O92_18970) nadC 3954523..3955416 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N5O92_RS18975 (N5O92_18975) pilA 3955619..3956059 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  N5O92_RS18980 (N5O92_18980) pilB 3956069..3957454 (+) 1386 WP_001025146.1 type II secretion system protein GspE Machinery gene
  N5O92_RS18985 (N5O92_18985) hofC 3957444..3958646 (+) 1203 WP_000157266.1 protein transport protein HofC -
  N5O92_RS18990 (N5O92_18990) guaC 3958681..3959724 (-) 1044 WP_001217338.1 GMP reductase -
  N5O92_RS18995 (N5O92_18995) - 3959880..3959924 (-) 45 WP_120795372.1 protein YacM -
  N5O92_RS19000 (N5O92_19000) coaE 3959949..3960569 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=632582 N5O92_RS18975 WP_000360904.1 3955619..3956059(+) (pilA) [Escherichia coli strain ECO3183]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=632582 N5O92_RS18975 WP_000360904.1 3955619..3956059(+) (pilA) [Escherichia coli strain ECO3183]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363