Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M5R25_RS20045 Genome accession   NZ_CP103657
Coordinates   4151939..4152379 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain 3150     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4146939..4157379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5R25_RS20025 (M5R25_20025) aroP 4147937..4149310 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  M5R25_RS20030 (M5R25_20030) ampE 4149353..4150207 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  M5R25_RS20035 (M5R25_20035) ampD 4150204..4150755 (-) 552 WP_022645179.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M5R25_RS20040 (M5R25_20040) nadC 4150843..4151736 (+) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M5R25_RS20045 (M5R25_20045) pilA 4151939..4152379 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  M5R25_RS20050 (M5R25_20050) pilB 4152389..4153774 (+) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  M5R25_RS20055 (M5R25_20055) hofC 4153764..4154966 (+) 1203 WP_000157236.1 protein transport protein HofC -
  M5R25_RS20060 (M5R25_20060) guaC 4155001..4156044 (-) 1044 WP_001217338.1 GMP reductase -
  M5R25_RS20065 (M5R25_20065) - 4156200..4156244 (-) 45 WP_120795372.1 protein YacM -
  M5R25_RS20070 (M5R25_20070) coaE 4156269..4156889 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=624073 M5R25_RS20045 WP_000360895.1 4151939..4152379(+) (pilA) [Escherichia coli strain 3150]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=624073 M5R25_RS20045 WP_000360895.1 4151939..4152379(+) (pilA) [Escherichia coli strain 3150]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363