Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   NML26_RS03890 Genome accession   NZ_CP101210
Coordinates   786606..787265 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain EC21Z-147     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 781606..792265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NML26_RS03865 (NML26_03865) parE 781658..783550 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  NML26_RS03870 (NML26_03870) ygiN 783598..783912 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  NML26_RS03875 (NML26_03875) mdaB 783943..784524 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  NML26_RS03880 (NML26_03880) ygiZ 784882..785214 (+) 333 WP_000912091.1 DUF2645 family protein -
  NML26_RS03885 (NML26_03885) qseC 785260..786609 (-) 1350 WP_000673348.1 quorum sensing histidine kinase QseC -
  NML26_RS03890 (NML26_03890) ciaR 786606..787265 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  NML26_RS03895 (NML26_03895) ygiW 787417..787809 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  NML26_RS03900 (NML26_03900) ygiV 787862..788344 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  NML26_RS03905 (NML26_03905) ygiS 788453..790060 (+) 1608 WP_001308070.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=609148 NML26_RS03890 WP_001221502.1 786606..787265(-) (ciaR) [Escherichia coli strain EC21Z-147]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=609148 NML26_RS03890 WP_001221502.1 786606..787265(-) (ciaR) [Escherichia coli strain EC21Z-147]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAACTGCGCCACGGGAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTG
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365