Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   NMD53_RS09400 Genome accession   NZ_CP100909
Coordinates   1914424..1915848 (-) Length   474 a.a.
NCBI ID   WP_085437461.1    Uniprot ID   -
Organism   Escherichia coli strain ET776     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1909424..1920848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD53_RS09370 (NMD53_09315) yeeZ 1909479..1910303 (+) 825 WP_000754758.1 SDR family oxidoreductase -
  NMD53_RS09375 (NMD53_09320) yeeY 1910349..1911278 (+) 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
  NMD53_RS09380 (NMD53_09325) yoeI 1911493..1911555 (+) 63 WP_010723108.1 membrane protein YoeI -
  NMD53_RS09385 (NMD53_09330) plaP 1911545..1912903 (+) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  NMD53_RS09390 (NMD53_09335) tsuA 1913082..1914140 (+) 1059 WP_001759318.1 thiosulfate utilization transporter TsuA/YeeE -
  NMD53_RS09395 (NMD53_09340) tsuB 1914154..1914381 (+) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  NMD53_RS09400 (NMD53_09345) sbcB 1914424..1915848 (-) 1425 WP_085437461.1 exodeoxyribonuclease I Machinery gene
  NMD53_RS09405 (NMD53_09350) - 1916030..1917208 (+) 1179 WP_066019383.1 site-specific integrase -
  NMD53_RS09410 (NMD53_09355) - 1917189..1917380 (-) 192 WP_000132739.1 AlpA family transcriptional regulator -
  NMD53_RS09415 (NMD53_09360) - 1917545..1917844 (-) 300 WP_021559316.1 hypothetical protein -
  NMD53_RS09420 (NMD53_09365) - 1917841..1918008 (-) 168 WP_066019382.1 DUF2737 family protein -
  NMD53_RS09425 (NMD53_09370) - 1918019..1918312 (-) 294 WP_016063329.1 phage anti-RecBCD protein -
  NMD53_RS09430 (NMD53_09375) ssb 1918326..1918832 (-) 507 WP_000168274.1 single-stranded DNA-binding protein -
  NMD53_RS09435 (NMD53_09380) - 1918833..1919540 (-) 708 WP_066019381.1 Rad52/Rad22 family DNA repair protein -
  NMD53_RS09440 (NMD53_09385) - 1919549..1919719 (-) 171 WP_171822469.1 hypothetical protein -
  NMD53_RS09445 (NMD53_09390) kil 1919795..1919947 (-) 153 WP_001243353.1 host cell division inhibitory peptide Kil -
  NMD53_RS09450 (NMD53_09395) - 1919932..1920033 (-) 102 WP_033559553.1 hypothetical protein -
  NMD53_RS09455 (NMD53_09400) - 1920082..1920174 (-) 93 Protein_1852 antitermination protein N -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 54443.68 Da        Isoelectric Point: 5.2416

>NTDB_id=606716 NMD53_RS09400 WP_085437461.1 1914424..1915848(-) (sbcB) [Escherichia coli strain ET776]
MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENE
AAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRG
NTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDAD
RLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDK
RIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDREKMALLKALWQYAEEIV

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=606716 NMD53_RS09400 WP_085437461.1 1914424..1915848(-) (sbcB) [Escherichia coli strain ET776]
ATGACAGACACAGATAAGCAACCTACCTTCCTCTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCC
TGCACAGTTCGCAGCCATTCGCACCGATGACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCGG
ATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCTCGGGCGAAAGGAGAAAACGAA
GCCGCATTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCAAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGA
CGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCT
GGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTA
CCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTA
CGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGCAATAAAC
ACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGGGCATGGCGCGGC
AACACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGACAT
TTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCG
CCGTTCCGGTTAAGCTGGTACACATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGAC
CGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGTGGT
GGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACG
CAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGATAAA
CGAATTGAAAAACTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCT
GGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTACAGGGTTATGCAGATGAATTGCAGATGCTGGCACAACAATATG
CCGATGACAGAGAGAAAATGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(12-187)

Exonuclease C-terminal (ExoI_SH3)

(211-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

59.362

99.156

0.589