Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   LSI69_RS00945 Genome accession   NZ_CP089032
Coordinates   175893..176561 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli O157:H7 strain 6535WT     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 170893..181561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSI69_RS00915 (LSI69_00915) yicC 170912..171775 (-) 864 WP_000621337.1 YicC/YloC family endoribonuclease -
  LSI69_RS00920 (LSI69_00920) rph 171902..172618 (+) 717 WP_001247093.1 ribonuclease PH -
  LSI69_RS00925 (LSI69_00925) pyrE 172684..173325 (+) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  LSI69_RS00930 (LSI69_00930) slmA 173362..173958 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  LSI69_RS00935 (LSI69_00935) dut 174065..174523 (-) 459 WP_000976070.1 dUTP diphosphatase -
  LSI69_RS00940 (LSI69_00940) coaBC 174501..175721 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  LSI69_RS00945 (LSI69_00945) radC 175893..176561 (+) 669 WP_001297375.1 RadC family protein Machinery gene
  LSI69_RS00950 (LSI69_00950) rpmB 176778..177014 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  LSI69_RS00955 (LSI69_00955) rpmG 177035..177202 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  LSI69_RS00960 (LSI69_00960) mutM 177300..178109 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  LSI69_RS00965 (LSI69_00965) coaD 178148..178627 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  LSI69_RS00970 (LSI69_00970) waaA 178635..179912 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  LSI69_RS00975 (LSI69_00975) rfaQ 180325..181383 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=547762 LSI69_RS00945 WP_001297375.1 175893..176561(+) (radC) [Escherichia coli O157:H7 strain 6535WT]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=547762 LSI69_RS00945 WP_001297375.1 175893..176561(+) (radC) [Escherichia coli O157:H7 strain 6535WT]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACTGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496