Detailed information    

insolico Bioinformatically predicted

Overview


Name   recA   Type   Machinery gene
Locus tag   JMW51_RS14175 Genome accession   NZ_CP068557
Coordinates   3112610..3113656 (-) Length   348 a.a.
NCBI ID   WP_003419530.1    Uniprot ID   A0AAX3GVV5
Organism   Clostridioides difficile strain CD4401     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 3071000..3138520 3112610..3113656 within 0


Gene organization within MGE regions


Location: 3071000..3138520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JMW51_RS13980 (JMW51_13980) - 3071273..3071695 (-) 423 WP_003419651.1 phage baseplate protein -
  JMW51_RS13985 (JMW51_13985) - 3071695..3074148 (-) 2454 WP_003419649.1 hypothetical protein -
  JMW51_RS13990 (JMW51_13990) - 3074342..3074788 (-) 447 WP_003419647.1 phage tail assembly chaperone -
  JMW51_RS13995 (JMW51_13995) - 3074902..3075330 (-) 429 WP_003419646.1 phage tail tube protein -
  JMW51_RS14000 (JMW51_14000) - 3075344..3076408 (-) 1065 WP_003419645.1 phage tail sheath subtilisin-like domain-containing protein -
  JMW51_RS14005 (JMW51_14005) - 3076413..3076856 (-) 444 WP_003419644.1 phage tail terminator family protein -
  JMW51_RS14010 (JMW51_14010) - 3077105..3077545 (-) 441 WP_003419643.1 hypothetical protein -
  JMW51_RS14015 (JMW51_14015) - 3077649..3077846 (-) 198 WP_003419641.1 helix-turn-helix transcriptional regulator -
  JMW51_RS14020 (JMW51_14020) - 3078015..3078335 (+) 321 WP_003419639.1 XRE family transcriptional regulator -
  JMW51_RS14025 (JMW51_14025) - 3078417..3078923 (+) 507 WP_003419636.1 ImmA/IrrE family metallo-endopeptidase -
  JMW51_RS14030 (JMW51_14030) - 3079624..3080379 (+) 756 WP_009889131.1 peptidylprolyl isomerase -
  JMW51_RS14035 (JMW51_14035) - 3080522..3080950 (-) 429 WP_009889129.1 CBS domain-containing protein -
  JMW51_RS14040 (JMW51_14040) - 3081371..3081571 (-) 201 WP_003419624.1 cold-shock protein -
  JMW51_RS14045 (JMW51_14045) - 3081754..3082908 (-) 1155 WP_009905420.1 amidase domain-containing protein -
  JMW51_RS14050 (JMW51_14050) - 3083159..3083980 (+) 822 WP_003419615.1 pyridoxamine kinase -
  JMW51_RS14055 (JMW51_14055) - 3084056..3085447 (-) 1392 WP_029158131.1 sensor histidine kinase -
  JMW51_RS14060 (JMW51_14060) - 3085581..3086351 (-) 771 WP_003419609.1 lantibiotic immunity ABC transporter MutG family permease subunit -
  JMW51_RS14065 (JMW51_14065) - 3086353..3087072 (-) 720 WP_003419603.1 lantibiotic immunity ABC transporter MutE/EpiE family permease subunit -
  JMW51_RS14070 (JMW51_14070) - 3087074..3087781 (-) 708 WP_009905418.1 lantibiotic protection ABC transporter ATP-binding protein -
  JMW51_RS14075 (JMW51_14075) - 3087901..3088437 (-) 537 WP_003419598.1 hypothetical protein -
  JMW51_RS14080 (JMW51_14080) - 3088539..3089609 (-) 1071 WP_003419596.1 DUF4097 family beta strand repeat-containing protein -
  JMW51_RS14085 (JMW51_14085) - 3089602..3090660 (-) 1059 WP_003419595.1 DUF1700 domain-containing protein -
  JMW51_RS14090 (JMW51_14090) - 3090647..3090979 (-) 333 WP_009905416.1 PadR family transcriptional regulator -
  JMW51_RS14095 (JMW51_14095) - 3091178..3092299 (+) 1122 WP_003419591.1 MGDG synthase family glycosyltransferase -
  JMW51_RS14100 (JMW51_14100) - 3092427..3094325 (-) 1899 WP_003419583.1 glutamine synthetase -
  JMW51_RS14105 (JMW51_14105) - 3094640..3095416 (+) 777 WP_003419578.1 3'-5' exonuclease -
  JMW51_RS14110 (JMW51_14110) - 3095508..3096260 (-) 753 WP_003419575.1 exodeoxyribonuclease III -
  JMW51_RS14115 (JMW51_14115) galT 3096381..3097331 (-) 951 WP_003419573.1 galactose-1-phosphate uridylyltransferase -
  JMW51_RS14120 (JMW51_14120) - 3097716..3098912 (+) 1197 WP_003419570.1 pyridoxal phosphate-dependent aminotransferase -
  JMW51_RS14125 (JMW51_14125) - 3099131..3099583 (-) 453 WP_003419561.1 MarR family winged helix-turn-helix transcriptional regulator -
  JMW51_RS14130 (JMW51_14130) purB 3099884..3101329 (-) 1446 WP_003419557.1 adenylosuccinate lyase -
  JMW51_RS14135 (JMW51_14135) - 3101468..3102379 (-) 912 WP_022615208.1 tyrosine-type recombinase/integrase -
  JMW51_RS14140 (JMW51_14140) - 3102636..3103460 (+) 825 WP_009905410.1 undecaprenyl-diphosphate phosphatase -
  JMW51_RS14145 (JMW51_14145) lepB 3103500..3104030 (+) 531 WP_009905409.1 signal peptidase I -
  JMW51_RS14150 (JMW51_14150) - 3104110..3105891 (-) 1782 WP_003419546.1 UvrD-helicase domain-containing protein -
  JMW51_RS14155 (JMW51_14155) tnpB 3106223..3107341 (-) 1119 WP_041160402.1 IS200/IS605 family element RNA-guided endonuclease TnpB -
  JMW51_RS14160 (JMW51_14160) - 3107343..3107441 (-) 99 Protein_2738 transposase -
  JMW51_RS14165 (JMW51_14165) - 3108247..3110583 (-) 2337 WP_003419542.1 diguanylate cyclase -
  JMW51_RS14170 (JMW51_14170) rny 3110893..3112434 (-) 1542 WP_003428216.1 ribonuclease Y -
  JMW51_RS14175 (JMW51_14175) recA 3112610..3113656 (-) 1047 WP_003419530.1 recombinase RecA Machinery gene
  JMW51_RS14180 (JMW51_14180) pgsA 3113923..3114465 (-) 543 WP_009905407.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  JMW51_RS14185 (JMW51_14185) rimO 3114452..3115786 (-) 1335 WP_003419527.1 30S ribosomal protein S12 methylthiotransferase RimO -
  JMW51_RS14190 (JMW51_14190) mnmH 3115780..3116838 (-) 1059 WP_003419524.1 tRNA 2-selenouridine(34) synthase MnmH -
  JMW51_RS14195 (JMW51_14195) - 3116841..3119249 (-) 2409 WP_003419521.1 FtsK/SpoIIIE family DNA translocase -
  JMW51_RS14200 (JMW51_14200) - 3119372..3120106 (-) 735 WP_009905406.1 ClpP family protease -
  JMW51_RS14205 (JMW51_14205) dapG 3120220..3121443 (-) 1224 WP_003419506.1 aspartate kinase -
  JMW51_RS14210 (JMW51_14210) - 3121453..3121698 (-) 246 WP_003419503.1 YlmC/YmxH family sporulation protein -
  JMW51_RS14215 (JMW51_14215) - 3121733..3122980 (-) 1248 WP_003419500.1 M16 family metallopeptidase -
  JMW51_RS14220 (JMW51_14220) - 3123138..3123881 (-) 744 WP_003419498.1 polysaccharide deacetylase family protein -
  JMW51_RS14225 (JMW51_14225) pnp 3124004..3126115 (-) 2112 WP_003419494.1 polyribonucleotide nucleotidyltransferase -
  JMW51_RS14230 (JMW51_14230) - 3126240..3127085 (-) 846 WP_003419493.1 DegV family protein -
  JMW51_RS14235 (JMW51_14235) rpsO 3127325..3127582 (-) 258 WP_003419491.1 30S ribosomal protein S15 -
  JMW51_RS14240 (JMW51_14240) - 3127686..3128615 (-) 930 WP_003419488.1 bifunctional riboflavin kinase/FAD synthetase -
  JMW51_RS14245 (JMW51_14245) truB 3128745..3129650 (-) 906 WP_003419486.1 tRNA pseudouridine(55) synthase TruB -
  JMW51_RS14250 (JMW51_14250) - 3129663..3130646 (-) 984 WP_003419484.1 DHH family phosphoesterase -
  JMW51_RS14255 (JMW51_14255) rbfA 3130627..3131007 (-) 381 WP_003419483.1 30S ribosome-binding factor RbfA -
  JMW51_RS14260 (JMW51_14260) infB 3131050..3132990 (-) 1941 WP_003419480.1 translation initiation factor IF-2 -
  JMW51_RS14265 (JMW51_14265) - 3133012..3133323 (-) 312 WP_003419470.1 L7Ae/L30e/S12e/Gadd45 family ribosomal protein -
  JMW51_RS14270 (JMW51_14270) rnpM 3133313..3133594 (-) 282 WP_003419469.1 RNase P modulator RnpM -
  JMW51_RS14275 (JMW51_14275) nusA 3133601..3134755 (-) 1155 WP_003419468.1 transcription termination factor NusA -
  JMW51_RS14280 (JMW51_14280) rimP 3134785..3135246 (-) 462 WP_003419467.1 ribosome maturation factor RimP -

Sequence


Protein


Download         Length: 348 a.a.        Molecular weight: 37387.68 Da        Isoelectric Point: 4.8236

>NTDB_id=529342 JMW51_RS14175 WP_003419530.1 3112610..3113656(-) (recA) [Clostridioides difficile strain CD4401]
MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTGAIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSC
VASAQKDGGIAAFIDAEHALDPVYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKFYSSVRLDVRKIDTIKQGDKVIG
SRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLLDIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTNEI
DEKVRAFYNLNEEHEEAGNSVSKEIVEE

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=529342 JMW51_RS14175 WP_003419530.1 3112610..3113656(-) (recA) [Clostridioides difficile strain CD4401]
ATGAGTGTAGATCAAGAAAAATTAAAAGCGTTGAATGAAGCTTTAGGTAAAATTGAAAAAGATTTTGGTAAAGGTTCAGT
AATGAAATTGGGAGAAGCAACATCTATGTCTATAGATGTTATATCAACAGGAGCGATTGGTTTAGATATAGCTATTGGTA
TAGGAGGTCTACCTAGAGGGAGAATAGTTGAAGTATATGGTCCAGAATCTTCTGGTAAGACTACTGTTGCACTTAGTTGT
GTAGCATCAGCTCAAAAAGATGGAGGAATAGCTGCATTTATAGATGCAGAACATGCACTTGACCCAGTATACGCAAAAGC
TTTGGGCGTGGATGTTGATAACCTAATAATATCTCAACCAGATACAGGTGAACAGGCTTTAGAGATAGCAGAGGCATTGA
TAAGAAGTGGAGCGATAGATATAATAGTAATAGACTCAGTAGCAGCATTAGTTCCAAAGGCTGAAATAGATGGAGATATG
GGTGACTCTCACGTAGGGTTACAAGCTAGACTTATGTCGCAAGCTCTTAGAAAGTTAACTGGTTCAATTAAAAAATCAAA
TTGTGTTGCTATATTTATAAACCAGTTAAGAGAGAAAGTAGGAATAATGTTTGGAAACCCAGAAACTACTACTGGAGGAC
GTGCATTAAAATTCTATTCATCAGTTAGATTGGATGTTAGAAAAATAGACACAATAAAACAAGGAGATAAAGTTATAGGT
AGTAGAACTAGAGTTAAAGTCGTTAAAAATAAAGTAGCACCTCCATTTAAGCAAGCTGAATTTGATATAATGTATGGAGA
AGGGATTTCAAAAATTGGAGACCTTCTAGATATAGCTGCTGATGTAGATATAGTAAAAAAATCAGGTTCATGGTATAGTT
ACAATGATACTAAACTTGGACAAGGAAGAGAAAATGTTAAAAAATTCTTGGAGGATAATTTAGATTTAACTAATGAAATA
GATGAGAAAGTTAGAGCATTTTACAATTTAAATGAAGAACATGAAGAAGCAGGTAATTCAGTATCAAAAGAAATTGTAGA
AGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Streptococcus mutans UA159

66.762

100

0.67

  recA Streptococcus mitis SK321

66.185

99.425

0.658

  recA Streptococcus mitis NCTC 12261

68.085

94.54

0.644

  recA Bacillus subtilis subsp. subtilis str. 168

69.565

92.529

0.644

  recA Streptococcus pneumoniae D39

67.173

94.54

0.635

  recA Streptococcus pneumoniae Rx1

67.173

94.54

0.635

  recA Streptococcus pneumoniae R6

67.173

94.54

0.635

  recA Streptococcus pneumoniae TIGR4

67.173

94.54

0.635

  recA Lactococcus lactis subsp. cremoris KW2

65.476

96.552

0.632

  recA Streptococcus pyogenes NZ131

66.972

93.966

0.629

  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

65.758

94.828

0.624

  recA Latilactobacillus sakei subsp. sakei 23K

64.955

95.115

0.618

  recA Neisseria gonorrhoeae MS11

66.987

89.655

0.601

  recA Neisseria gonorrhoeae MS11

66.987

89.655

0.601

  recA Neisseria gonorrhoeae strain FA1090

66.987

89.655

0.601

  recA Acinetobacter baylyi ADP1

64.087

92.816

0.595

  recA Pseudomonas stutzeri DSM 10701

63.889

93.103

0.595

  recA Helicobacter pylori 26695

61.976

95.977

0.595

  recA Helicobacter pylori strain NCTC11637

61.976

95.977

0.595

  recA Acinetobacter baumannii D1279779

63.777

92.816

0.592

  recA Vibrio cholerae O1 biovar El Tor strain E7946

62.048

95.402

0.592

  recA Vibrio cholerae strain A1552

62.048

95.402

0.592

  recA Glaesserella parasuis strain SC1401

64.174

92.241

0.592

  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

63.272

93.103

0.589

  recA Ralstonia pseudosolanacearum GMI1000

66.558

88.506

0.589

  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

60.681

92.816

0.563