Detailed information NTG0528057

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BAJP3144_RS13040
• Genome accession: NZ_CP082283
• Coordinates: 2668221..2668835 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain JP3144
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2663221..2673835

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BAJP3144_RS13010 (BAJP3144_13010)	rpsT	2663525..2663791 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BAJP3144_RS13015 (BAJP3144_13015)	holA	2663808..2664849 (-)	1042	Protein_2548	DNA polymerase III subunit delta	-
BAJP3144_RS13020 (BAJP3144_13020)	-	2664889..2665039 (-)	151	Protein_2549	hypothetical protein	-
BAJP3144_RS13025 (BAJP3144_13025)	-	2665080..2665214 (+)	135	WP_003152870.1	YqzM family protein	-
BAJP3144_RS13030 (BAJP3144_13030)	comEC	2665233..2667584 (-)	2352	WP_174747051.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BAJP3144_RS13035 (BAJP3144_13035)	-	2667585..2668154 (-)	570	WP_003152868.1	ComE operon protein 2	-
BAJP3144_RS13040 (BAJP3144_13040)	comEA	2668221..2668835 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
BAJP3144_RS13045 (BAJP3144_13045)	comER	2668894..2669715 (+)	822	WP_014418420.1	late competence protein ComER	-
BAJP3144_RS13050 (BAJP3144_13050)	-	2669784..2670521 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
BAJP3144_RS13055 (BAJP3144_13055)	rsfS	2670518..2670874 (-)	357	WP_007408260.1	ribosome silencing factor	-
BAJP3144_RS13060 (BAJP3144_13060)	yqeK	2670892..2671452 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BAJP3144_RS13065 (BAJP3144_13065)	-	2671442..2672011 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
BAJP3144_RS13070 (BAJP3144_13070)	yhbY	2672022..2672312 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BAJP3144_RS13075 (BAJP3144_13075)	aroE	2672306..2673148 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=528057 BAJP3144_RS13040 WP_014418419.1 2668221..2668835(-) (comEA) [Bacillus amyloliquefaciens strain JP3144]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=528057 BAJP3144_RS13040 WP_014418419.1 2668221..2668835(-) (comEA) [Bacillus amyloliquefaciens strain JP3144]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterProScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363