Detailed information    

insolico Bioinformatically predicted

Overview


Name   recA   Type   Machinery gene
Locus tag   HO397_RS12830 Genome accession   NZ_CP053601
Coordinates   2633134..2634195 (-) Length   353 a.a.
NCBI ID   WP_000963143.1    Uniprot ID   A0AAV3H3S5
Organism   Escherichia coli BL21     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2592984..2641205 2633134..2634195 within 0


Gene organization within MGE regions


Location: 2592984..2641205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HO397_RS12610 (HO397_12610) - 2592984..2594213 (+) 1230 WP_001062342.1 integrase domain-containing protein -
  HO397_RS12615 (HO397_12615) - 2594496..2596052 (+) 1557 WP_000135616.1 recombinase family protein -
  HO397_RS12620 (HO397_12620) - 2596042..2596938 (+) 897 WP_000936465.1 ParB/RepB/Spo0J family partition protein -
  HO397_RS12625 (HO397_12625) - 2596935..2597831 (+) 897 WP_000614784.1 plasmid partitioning protein RepB C-terminal domain-containing protein -
  HO397_RS12630 (HO397_12630) - 2598230..2598927 (+) 698 WP_094096600.1 IS1-like element IS1A family transposase -
  HO397_RS12635 (HO397_12635) glaH 2599233..2600210 (+) 978 WP_000993087.1 glutarate dioxygenase GlaH -
  HO397_RS12640 (HO397_12640) lhgO 2600229..2601497 (+) 1269 WP_000271963.1 L-2-hydroxyglutarate oxidase -
  HO397_RS12645 (HO397_12645) gabD 2601520..2602968 (+) 1449 WP_000772820.1 NADP-dependent succinate-semialdehyde dehydrogenase -
  HO397_RS12650 (HO397_12650) gabT 2602982..2604262 (+) 1281 WP_000097642.1 4-aminobutyrate--2-oxoglutarate transaminase -
  HO397_RS12655 (HO397_12655) gabP 2604573..2605973 (+) 1401 WP_001301367.1 GABA permease -
  HO397_RS12660 (HO397_12660) csiR 2605994..2606656 (+) 663 WP_000156811.1 DNA-binding transcriptional regulator CsiR -
  HO397_RS12665 (HO397_12665) kbp 2606657..2607106 (-) 450 WP_000522424.1 potassium binding protein Kbp -
  HO397_RS12670 (HO397_12670) yqaE 2607190..2607348 (-) 159 WP_000508177.1 YqaE/Pmp3 family membrane protein -
  HO397_RS12675 (HO397_12675) ygaV 2607531..2607830 (+) 300 WP_000137280.1 metalloregulator ArsR/SmtB family transcription factor -
  HO397_RS12680 (HO397_12680) ygaP 2607840..2608364 (+) 525 WP_001229445.1 thiosulfate sulfurtransferase YgaP -
  HO397_RS12685 (HO397_12685) stpA 2608411..2608815 (-) 405 WP_000115383.1 DNA-binding protein StpA -
  HO397_RS12690 (HO397_12690) alaE 2609483..2609932 (+) 450 WP_000492656.1 L-alanine exporter AlaE -
  HO397_RS12695 (HO397_12695) ygaC 2609969..2610313 (-) 345 WP_000281320.1 DUF2002 family protein -
  HO397_RS12700 (HO397_12700) ygaM 2610465..2610794 (+) 330 WP_001295174.1 DUF883 domain-containing protein -
  HO397_RS12705 (HO397_12705) nrdH 2611042..2611287 (+) 246 WP_001223227.1 glutaredoxin-like protein NrdH -
  HO397_RS12710 (HO397_12710) nrdI 2611284..2611694 (+) 411 WP_000080947.1 class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI -
  HO397_RS12715 (HO397_12715) nrdE 2611667..2613811 (+) 2145 WP_000246527.1 class 1b ribonucleoside-diphosphate reductase subunit alpha -
  HO397_RS12720 (HO397_12720) nrdF 2613821..2614780 (+) 960 WP_000777972.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
  HO397_RS12725 (HO397_12725) proV 2615134..2616336 (+) 1203 WP_000985494.1 glycine betaine/L-proline ABC transporter ATP-binding protein ProV -
  HO397_RS12730 (HO397_12730) proW 2616329..2617393 (+) 1065 WP_000774988.1 glycine betaine/L-proline ABC transporter permease ProW -
  HO397_RS12735 (HO397_12735) proX 2617451..2618443 (+) 993 WP_001216521.1 glycine betaine/L-proline ABC transporter substrate-binding protein ProX -
  HO397_RS12740 (HO397_12740) - 2618735..2619919 (+) 1185 WP_000165699.1 MFS transporter -
  HO397_RS12745 (HO397_12745) ygaZ 2620043..2620780 (+) 738 WP_000445651.1 L-valine exporter subunit YgaZ -
  HO397_RS12750 (HO397_12750) ygaH 2620770..2621105 (+) 336 WP_000119763.1 L-valine transporter subunit YgaH -
  HO397_RS12755 (HO397_12760) emrR 2621196..2621726 (+) 531 WP_000378442.1 multidrug efflux transporter EmrAB transcriptional repressor EmrR -
  HO397_RS12760 (HO397_12765) emrA 2621853..2623025 (+) 1173 WP_001295175.1 multidrug efflux MFS transporter periplasmic adaptor subunit EmrA -
  HO397_RS12765 (HO397_12770) emrB 2623042..2624580 (+) 1539 WP_001295176.1 multidrug efflux MFS transporter permease subunit EmrB -
  HO397_RS12770 (HO397_12775) luxS 2624644..2625159 (-) 516 WP_001130211.1 S-ribosylhomocysteine lyase -
  HO397_RS12775 (HO397_12780) gshA 2625309..2626865 (-) 1557 WP_000611804.1 glutamate--cysteine ligase -
  HO397_RS12780 (HO397_12785) yqaA 2626938..2627366 (-) 429 WP_001287454.1 YqaA family protein -
  HO397_RS12785 (HO397_12790) yqaB 2627363..2627929 (-) 567 WP_000273290.1 fructose-1-phosphate/6-phosphogluconate phosphatase -
  HO397_RS12815 (HO397_12820) csrA 2629387..2629572 (-) 186 WP_000906486.1 carbon storage regulator CsrA -
  HO397_RS12820 (HO397_12825) alaS 2629807..2632437 (-) 2631 WP_000047185.1 alanine--tRNA ligase -
  HO397_RS12825 (HO397_12830) recX 2632565..2633065 (-) 501 WP_000140508.1 recombination regulator RecX -
  HO397_RS12830 (HO397_12835) recA 2633134..2634195 (-) 1062 WP_000963143.1 recombinase RecA Machinery gene
  HO397_RS12835 (HO397_12840) pncC 2634275..2634772 (-) 498 WP_000132231.1 nicotinamide-nucleotide amidase -
  HO397_RS12840 (HO397_12845) mltB 2634917..2636002 (-) 1086 WP_001301333.1 lytic murein transglycosylase B -
  HO397_RS12845 (HO397_12850) srlA 2636258..2636821 (+) 564 WP_000573321.1 PTS glucitol/sorbitol transporter subunit IIC -
  HO397_RS12850 (HO397_12855) srlE 2636818..2637777 (+) 960 WP_000148867.1 PTS glucitol/sorbitol transporter subunit IIB -
  HO397_RS12855 (HO397_12860) srlB 2637788..2638159 (+) 372 WP_000216210.1 PTS glucitol/sorbitol transporter subunit IIA -
  HO397_RS12860 (HO397_12865) srlD 2638163..2638942 (+) 780 WP_001077351.1 sorbitol-6-phosphate dehydrogenase -
  HO397_RS12865 (HO397_12870) gutM 2639048..2639407 (+) 360 WP_000252907.1 transcriptional regulator GutM -
  HO397_RS12870 (HO397_12875) srlR 2639474..2640247 (+) 774 WP_000804550.1 glucitol operon DNA-binding transcriptional repressor SrlR -
  HO397_RS12875 (HO397_12880) gutQ 2640240..2641205 (+) 966 WP_001287420.1 arabinose-5-phosphate isomerase GutQ -

Sequence


Protein


Download         Length: 353 a.a.        Molecular weight: 37973.37 Da        Isoelectric Point: 4.8171

>NTDB_id=445414 HO397_RS12830 WP_000963143.1 2633134..2634195(-) (recA) [Escherichia coli BL21]
MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTGSLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQV
IAAAQREGKTCAFIDAEHALDPIYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRIGAVKEGENVVG
SETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELVDLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEI
EKKVRELLLSNPNSTPDFSVDDSEGVAETNEDF

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=445414 HO397_RS12830 WP_000963143.1 2633134..2634195(-) (recA) [Escherichia coli BL21]
ATGGCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGATTGAGAAACAATTTGGTAAAGGCTCCAT
CATGCGCCTGGGTGAAGACCGTTCCATGGATGTGGAAACCATCTCTACCGGTTCGCTTTCACTGGATATCGCGCTTGGGG
CAGGTGGTCTGCCGATGGGCCGTATCGTCGAAATCTACGGACCGGAATCTTCCGGTAAAACCACGCTGACGCTGCAGGTG
ATCGCCGCAGCGCAGCGTGAAGGTAAAACCTGTGCGTTTATCGATGCTGAACACGCGCTGGACCCAATCTACGCACGTAA
ACTGGGCGTCGATATCGATAACCTGCTGTGCTCCCAGCCGGACACCGGCGAGCAGGCACTGGAAATCTGTGACGCCCTGG
CGCGTTCTGGCGCAGTAGACGTTATCGTCGTTGACTCCGTGGCGGCACTGACGCCGAAAGCGGAAATCGAAGGCGAAATC
GGCGACTCTCACATGGGCCTTGCGGCACGTATGATGAGCCAGGCGATGCGTAAGCTGGCGGGTAACCTGAAGCAGTCCAA
CACGCTGCTGATCTTCATCAACCAGATCCGTATGAAAATTGGTGTGATGTTCGGTAACCCGGAAACCACTACCGGTGGTA
ACGCGCTGAAATTCTACGCCTCTGTTCGTCTCGACATCCGTCGTATCGGCGCGGTGAAAGAGGGCGAAAACGTGGTGGGT
AGCGAAACCCGCGTGAAAGTGGTGAAGAACAAAATCGCTGCGCCGTTTAAACAGGCTGAATTCCAGATCCTCTACGGCGA
AGGTATCAACTTCTACGGCGAACTGGTTGACCTGGGCGTAAAAGAGAAGCTGATCGAGAAAGCAGGCGCGTGGTACAGCT
ACAAAGGTGAGAAGATCGGTCAGGGTAAAGCGAATGCGACTGCCTGGCTGAAAGATAACCCGGAAACCGCGAAAGAGATC
GAGAAGAAAGTACGTGAGTTGCTGCTGAGCAACCCGAACTCAACGCCGGATTTCTCTGTAGATGATAGCGAAGGCGTAGC
AGAAACTAACGAAGATTTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Vibrio cholerae O1 biovar El Tor strain E7946

84.29

93.768

0.79

  recA Vibrio cholerae strain A1552

84.29

93.768

0.79

  recA Pseudomonas stutzeri DSM 10701

74.184

95.467

0.708

  recA Acinetobacter baumannii D1279779

74.085

92.918

0.688

  recA Acinetobacter baylyi ADP1

73.78

92.918

0.686

  recA Glaesserella parasuis strain SC1401

69.452

98.3

0.683

  recA Neisseria gonorrhoeae MS11

69.018

92.351

0.637

  recA Neisseria gonorrhoeae MS11

69.018

92.351

0.637

  recA Neisseria gonorrhoeae strain FA1090

69.018

92.351

0.637

  recA Ralstonia pseudosolanacearum GMI1000

70.159

89.235

0.626

  recA Streptococcus mitis SK321

60.286

99.15

0.598

  recA Streptococcus mitis NCTC 12261

60.286

99.15

0.598

  recA Helicobacter pylori strain NCTC11637

61.721

95.467

0.589

  recA Helicobacter pylori 26695

61.424

95.467

0.586

  recA Streptococcus pneumoniae TIGR4

63.467

91.501

0.581

  recA Streptococcus pneumoniae Rx1

63.467

91.501

0.581

  recA Streptococcus pneumoniae D39

63.467

91.501

0.581

  recA Streptococcus pneumoniae R6

63.467

91.501

0.581

  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

62.654

91.785

0.575

  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

60.661

94.334

0.572

  recA Streptococcus mutans UA159

62.154

92.068

0.572

  recA Streptococcus pyogenes NZ131

62.154

92.068

0.572

  recA Bacillus subtilis subsp. subtilis str. 168

62.305

90.935

0.567

  recA Latilactobacillus sakei subsp. sakei 23K

61.538

92.068

0.567

  recA Lactococcus lactis subsp. cremoris KW2

61.61

91.501

0.564

  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

61.059

90.935

0.555