Detailed information    

insolico Bioinformatically predicted

Overview


Name   recA   Type   Machinery gene
Locus tag   FOC31_RS07345 Genome accession   NZ_CP046327
Coordinates   1601089..1602135 (-) Length   348 a.a.
NCBI ID   WP_003428218.1    Uniprot ID   A0A031WFI0
Organism   Clostridioides difficile strain FDAARGOS_723     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1552326..1634067 1601089..1602135 within 0


Gene organization within MGE regions


Location: 1552326..1634067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOC31_RS07110 (FOC31_07110) - 1554494..1555480 (-) 987 WP_009889153.1 phage tail protein -
  FOC31_RS07115 (FOC31_07115) - 1555481..1556179 (-) 699 WP_009889149.1 putative phage tail protein -
  FOC31_RS07120 (FOC31_07120) - 1556176..1557228 (-) 1053 WP_009889148.1 baseplate J/gp47 family protein -
  FOC31_RS07125 (FOC31_07125) - 1557221..1557649 (-) 429 WP_003428161.1 DUF2634 domain-containing protein -
  FOC31_RS07130 (FOC31_07130) - 1557649..1557975 (-) 327 WP_003438447.1 DUF2577 domain-containing protein -
  FOC31_RS07135 (FOC31_07135) - 1557991..1559520 (-) 1530 WP_009889145.1 NlpC/P60 family protein -
  FOC31_RS07140 (FOC31_07140) - 1559802..1560224 (-) 423 WP_009889143.1 phage baseplate protein -
  FOC31_RS07145 (FOC31_07145) - 1560224..1562677 (-) 2454 WP_009889141.1 hypothetical protein -
  FOC31_RS07150 (FOC31_07150) - 1562677..1562844 (-) 168 WP_003419648.1 hypothetical protein -
  FOC31_RS07155 (FOC31_07155) - 1562871..1563317 (-) 447 WP_009889139.1 phage tail assembly chaperone -
  FOC31_RS07160 (FOC31_07160) - 1563431..1563859 (-) 429 WP_003419646.1 phage tail tube protein -
  FOC31_RS07165 (FOC31_07165) - 1563873..1564937 (-) 1065 WP_009889137.1 phage tail sheath subtilisin-like domain-containing protein -
  FOC31_RS07170 (FOC31_07170) - 1564942..1565385 (-) 444 WP_009889135.1 phage tail terminator family protein -
  FOC31_RS07175 (FOC31_07175) - 1565634..1566074 (-) 441 WP_009889133.1 hypothetical protein -
  FOC31_RS07180 (FOC31_07180) - 1566179..1566376 (-) 198 WP_003419641.1 helix-turn-helix transcriptional regulator -
  FOC31_RS07185 (FOC31_07185) - 1566545..1566865 (+) 321 WP_003428185.1 XRE family transcriptional regulator -
  FOC31_RS07190 (FOC31_07190) - 1566947..1567453 (+) 507 WP_003428186.1 ImmA/IrrE family metallo-endopeptidase -
  FOC31_RS07200 (FOC31_07200) - 1568154..1568909 (+) 756 WP_009889131.1 peptidylprolyl isomerase -
  FOC31_RS07205 (FOC31_07205) - 1569052..1569480 (-) 429 WP_009889129.1 CBS domain-containing protein -
  FOC31_RS07210 (FOC31_07210) - 1569901..1570101 (-) 201 WP_003419624.1 cold-shock protein -
  FOC31_RS07215 (FOC31_07215) - 1570284..1571438 (-) 1155 WP_009892989.1 amidase domain-containing protein -
  FOC31_RS07220 (FOC31_07220) - 1571689..1572510 (+) 822 WP_003428191.1 pyridoxamine kinase -
  FOC31_RS07225 (FOC31_07225) - 1572586..1573974 (-) 1389 WP_009889126.1 two-component system sensor histidine kinase -
  FOC31_RS07230 (FOC31_07230) - 1574118..1574876 (-) 759 WP_009889124.1 lantibiotic immunity ABC transporter MutG family permease subunit -
  FOC31_RS07235 (FOC31_07235) - 1574878..1575594 (-) 717 WP_009889122.1 lantibiotic immunity ABC transporter MutE/EpiE family permease subunit -
  FOC31_RS07240 (FOC31_07240) - 1575596..1576303 (-) 708 WP_009889121.1 lantibiotic protection ABC transporter ATP-binding protein -
  FOC31_RS07245 (FOC31_07245) - 1576420..1576956 (-) 537 WP_003428196.1 lipoprotein -
  FOC31_RS07250 (FOC31_07250) - 1577058..1578128 (-) 1071 WP_009889118.1 DUF4097 family beta strand repeat-containing protein -
  FOC31_RS07255 (FOC31_07255) - 1578121..1579179 (-) 1059 WP_012816112.1 DUF1700 domain-containing protein -
  FOC31_RS07260 (FOC31_07260) - 1579166..1579498 (-) 333 WP_009889116.1 PadR family transcriptional regulator -
  FOC31_RS07265 (FOC31_07265) - 1579697..1580818 (+) 1122 WP_003428201.1 MGDG synthase family glycosyltransferase -
  FOC31_RS07270 (FOC31_07270) - 1580946..1582844 (-) 1899 WP_003428202.1 glutamine synthetase -
  FOC31_RS07275 (FOC31_07275) - 1583159..1583935 (+) 777 WP_009889114.1 3'-5' exonuclease -
  FOC31_RS07280 (FOC31_07280) - 1584027..1584779 (-) 753 WP_003438367.1 exodeoxyribonuclease III -
  FOC31_RS07285 (FOC31_07285) galT 1584940..1585890 (-) 951 WP_009889113.1 galactose-1-phosphate uridylyltransferase -
  FOC31_RS07290 (FOC31_07290) - 1586275..1587471 (+) 1197 WP_003438357.1 pyridoxal phosphate-dependent aminotransferase -
  FOC31_RS07295 (FOC31_07295) - 1587583..1588929 (-) 1347 WP_009892979.1 6-phospho-alpha-glucosidase -
  FOC31_RS07300 (FOC31_07300) - 1589062..1589814 (-) 753 WP_009892977.1 MurR/RpiR family transcriptional regulator -
  FOC31_RS07305 (FOC31_07305) - 1589973..1591550 (-) 1578 WP_009889112.1 PTS transporter subunit EIIC -
  FOC31_RS07310 (FOC31_07310) - 1591963..1592415 (-) 453 WP_003419561.1 MarR family winged helix-turn-helix transcriptional regulator -
  FOC31_RS07315 (FOC31_07315) purB 1592726..1594171 (-) 1446 WP_009889111.1 adenylosuccinate lyase -
  FOC31_RS07320 (FOC31_07320) - 1594315..1595223 (-) 909 WP_009889110.1 tyrosine-type recombinase/integrase -
  FOC31_RS07325 (FOC31_07325) - 1595480..1596304 (+) 825 WP_009889109.1 undecaprenyl-diphosphate phosphatase -
  FOC31_RS07330 (FOC31_07330) lepB 1596344..1596874 (+) 531 WP_009889108.1 signal peptidase I -
  FOC31_RS07335 (FOC31_07335) - 1596956..1598737 (-) 1782 WP_003438344.1 UvrD-helicase domain-containing protein -
  FOC31_RS07340 (FOC31_07340) rny 1599372..1600913 (-) 1542 WP_003428216.1 ribonuclease Y -
  FOC31_RS07345 (FOC31_07345) recA 1601089..1602135 (-) 1047 WP_003428218.1 recombinase RecA Machinery gene
  FOC31_RS07350 (FOC31_07350) pgsA 1602423..1602965 (-) 543 WP_009889106.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  FOC31_RS07355 (FOC31_07355) rimO 1602952..1604286 (-) 1335 WP_009892976.1 30S ribosomal protein S12 methylthiotransferase RimO -
  FOC31_RS07360 (FOC31_07360) mnmH 1604280..1605338 (-) 1059 WP_009889105.1 tRNA 2-selenouridine(34) synthase MnmH -
  FOC31_RS07365 (FOC31_07365) - 1605341..1607752 (-) 2412 WP_009889104.1 FtsK/SpoIIIE family DNA translocase -
  FOC31_RS07370 (FOC31_07370) - 1607875..1608609 (-) 735 WP_003428224.1 ClpP family protease -
  FOC31_RS07375 (FOC31_07375) dapG 1608722..1609945 (-) 1224 WP_009889103.1 aspartate kinase -
  FOC31_RS07380 (FOC31_07380) - 1609955..1610200 (-) 246 WP_003419503.1 YlmC/YmxH family sporulation protein -
  FOC31_RS07385 (FOC31_07385) - 1610235..1611482 (-) 1248 WP_009889101.1 M16 family metallopeptidase -
  FOC31_RS07390 (FOC31_07390) - 1611640..1612383 (-) 744 WP_003438319.1 polysaccharide deacetylase family protein -
  FOC31_RS07395 (FOC31_07395) pnp 1612649..1614760 (-) 2112 WP_003438316.1 polyribonucleotide nucleotidyltransferase -
  FOC31_RS07400 (FOC31_07400) - 1614885..1615730 (-) 846 WP_009889099.1 DegV family protein -
  FOC31_RS07405 (FOC31_07405) rpsO 1615970..1616227 (-) 258 WP_003419491.1 30S ribosomal protein S15 -
  FOC31_RS07415 (FOC31_07415) - 1616331..1617260 (-) 930 WP_009889098.1 bifunctional riboflavin kinase/FAD synthetase -
  FOC31_RS07420 (FOC31_07420) truB 1617391..1618296 (-) 906 WP_009889097.1 tRNA pseudouridine(55) synthase TruB -
  FOC31_RS07425 (FOC31_07425) - 1618309..1619292 (-) 984 WP_095890265.1 DHH family phosphoesterase -
  FOC31_RS07430 (FOC31_07430) rbfA 1619273..1619653 (-) 381 WP_009889095.1 30S ribosome-binding factor RbfA -
  FOC31_RS07435 (FOC31_07435) infB 1619696..1621636 (-) 1941 WP_003438306.1 translation initiation factor IF-2 -
  FOC31_RS07440 (FOC31_07440) - 1621658..1621969 (-) 312 WP_003419470.1 L7Ae/L30e/S12e/Gadd45 family ribosomal protein -
  FOC31_RS07445 (FOC31_07445) rnpM 1621959..1622240 (-) 282 WP_003419469.1 RNase P modulator RnpM -
  FOC31_RS07450 (FOC31_07450) nusA 1622247..1623401 (-) 1155 WP_009889093.1 transcription termination factor NusA -
  FOC31_RS07455 (FOC31_07455) rimP 1623431..1623892 (-) 462 WP_003438303.1 ribosome maturation factor RimP -
  FOC31_RS07460 (FOC31_07460) - 1624096..1628394 (-) 4299 WP_009892973.1 PolC-type DNA polymerase III -
  FOC31_RS07465 (FOC31_07465) acd 1628609..1630432 (-) 1824 WP_009892971.1 N-acetylglucosaminidase -
  FOC31_RS07470 (FOC31_07470) - 1630647..1631384 (+) 738 WP_003428245.1 NAD-dependent protein deacylase -
  FOC31_RS07475 (FOC31_07475) - 1631504..1631824 (+) 321 WP_003428248.1 MazG-like family protein -
  FOC31_RS07480 (FOC31_07480) - 1631999..1632139 (-) 141 WP_003419462.1 hypothetical protein -
  FOC31_RS07485 (FOC31_07485) - 1632408..1633076 (+) 669 WP_009892970.1 YwaF family protein -

Sequence


Protein


Download         Length: 348 a.a.        Molecular weight: 37377.68 Da        Isoelectric Point: 4.8236

>NTDB_id=402643 FOC31_RS07345 WP_003428218.1 1601089..1602135(-) (recA) [Clostridioides difficile strain FDAARGOS_723]
MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTGAIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSC
VASAQKDGGIAAFIDAEHALDPVYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKFYSSVRLDVRKIDTIKQGDKVIG
SRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLLDIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEI
DEKVRAFYNLNEEHEESGTSVSKEIVEE

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=402643 FOC31_RS07345 WP_003428218.1 1601089..1602135(-) (recA) [Clostridioides difficile strain FDAARGOS_723]
ATGAGTGTAGATCAAGAAAAATTAAAAGCGTTGAATGAAGCTTTAGGTAAAATTGAAAAAGATTTTGGTAAAGGTTCAGT
AATGAAATTGGGAGAAGCAACATCTATGTCTATAGATGTTATATCAACAGGAGCGATTGGTTTAGACATAGCTATTGGTA
TAGGAGGTCTACCTAGAGGGAGAATAGTTGAAGTATATGGTCCAGAATCTTCTGGTAAGACTACTGTTGCGCTTAGTTGT
GTAGCATCAGCTCAAAAAGATGGAGGAATAGCTGCATTTATAGATGCAGAACATGCACTTGACCCAGTATATGCAAAAGC
TTTGGGTGTGGATGTTGATAACCTAATAATATCTCAACCAGATACAGGTGAACAGGCTTTAGAGATAGCAGAGGCATTGA
TAAGAAGTGGAGCGATAGATATAATAGTAATAGACTCAGTAGCAGCATTAGTTCCAAAGGCTGAAATAGATGGAGATATG
GGTGATTCTCATGTAGGTTTACAAGCTAGACTTATGTCACAAGCACTTAGAAAGTTAACTGGTTCAATTAAAAAATCAAA
TTGTGTTGCTATATTTATAAACCAGTTAAGAGAGAAAGTAGGAATAATGTTTGGAAACCCAGAAACTACTACTGGAGGAC
GTGCACTAAAATTCTATTCATCAGTTAGATTGGATGTTAGAAAAATAGATACAATAAAACAAGGTGATAAAGTTATAGGT
AGTAGAACTAGAGTTAAAGTTGTTAAAAACAAAGTAGCACCACCATTTAAGCAAGCTGAATTTGATATAATGTATGGAGA
AGGGATTTCAAAAATTGGAGACCTTTTAGATATAGCTGCTGATGTAGATATAGTTAAAAAATCAGGTTCATGGTATAGTT
ACAATGATACTAAACTTGGACAAGGAAGAGAAAATGTTAAAAAATTCTTGGAAGATAATTTAGATTTAACTACTGAAATA
GATGAGAAAGTTAGAGCATTTTACAATTTAAATGAAGAACATGAAGAATCAGGTACTTCAGTATCAAAAGAAATTGTAGA
AGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A031WFI0

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Streptococcus mutans UA159

66.762

100

0.67

  recA Streptococcus mitis SK321

67.456

97.126

0.655

  recA Streptococcus pneumoniae Rx1

65.507

99.138

0.649

  recA Streptococcus pneumoniae R6

65.507

99.138

0.649

  recA Streptococcus pneumoniae D39

65.507

99.138

0.649

  recA Streptococcus pneumoniae TIGR4

65.507

99.138

0.649

  recA Streptococcus mitis NCTC 12261

68.085

94.54

0.644

  recA Bacillus subtilis subsp. subtilis str. 168

69.565

92.529

0.644

  recA Lactococcus lactis subsp. cremoris KW2

65.569

95.977

0.629

  recA Streptococcus pyogenes NZ131

66.972

93.966

0.629

  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

65.758

94.828

0.624

  recA Latilactobacillus sakei subsp. sakei 23K

64.955

95.115

0.618

  recA Neisseria gonorrhoeae MS11

66.049

93.103

0.615

  recA Neisseria gonorrhoeae MS11

66.049

93.103

0.615

  recA Neisseria gonorrhoeae strain FA1090

66.049

93.103

0.615

  recA Acinetobacter baylyi ADP1

64.396

92.816

0.598

  recA Pseudomonas stutzeri DSM 10701

63.889

93.103

0.595

  recA Helicobacter pylori 26695

61.976

95.977

0.595

  recA Helicobacter pylori strain NCTC11637

61.976

95.977

0.595

  recA Acinetobacter baumannii D1279779

63.777

92.816

0.592

  recA Vibrio cholerae O1 biovar El Tor strain E7946

62.048

95.402

0.592

  recA Vibrio cholerae strain A1552

62.048

95.402

0.592

  recA Glaesserella parasuis strain SC1401

64.174

92.241

0.592

  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

63.272

93.103

0.589

  recA Ralstonia pseudosolanacearum GMI1000

66.558

88.506

0.589

  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

60.681

92.816

0.563


Multiple sequence alignment