Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G6G93_RS16975 Genome accession   NZ_CP049118
Coordinates   3527907..3529292 (-) Length   461 a.a.
NCBI ID   WP_058905579.1    Uniprot ID   -
Organism   Escherichia coli strain EC931     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3522907..3534292
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G6G93_RS16940 (G6G93_16980) mutT 3523237..3523626 (+) 390 WP_000736007.1 8-oxo-dGTP diphosphatase MutT -
  G6G93_RS16945 (G6G93_16985) yacG 3523842..3524039 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  G6G93_RS16950 (G6G93_16990) zapD 3524049..3524792 (-) 744 WP_001194734.1 cell division protein ZapD -
  G6G93_RS16955 (G6G93_16995) coaE 3524792..3525412 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  G6G93_RS16960 - 3525437..3525481 (+) 45 WP_120795372.1 protein YacM -
  G6G93_RS16965 (G6G93_17000) guaC 3525637..3526680 (+) 1044 WP_001217335.1 GMP reductase -
  G6G93_RS16970 (G6G93_17005) hofC 3526715..3527917 (-) 1203 WP_000157266.1 protein transport protein HofC -
  G6G93_RS16975 (G6G93_17010) pilB 3527907..3529292 (-) 1386 WP_058905579.1 type II secretion system protein GspE Machinery gene
  G6G93_RS16980 (G6G93_17015) pilA 3529302..3529742 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  G6G93_RS16985 (G6G93_17020) nadC 3529945..3530838 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G6G93_RS16990 (G6G93_17025) ampD 3530926..3531477 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G6G93_RS16995 (G6G93_17030) ampE 3531474..3532328 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  G6G93_RS17000 (G6G93_17035) aroP 3532371..3533744 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50605.29 Da        Isoelectric Point: 6.6612

>NTDB_id=372680 G6G93_RS16975 WP_058905579.1 3527907..3529292(-) (pilB) [Escherichia coli strain EC931]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQS
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=372680 G6G93_RS16975 WP_058905579.1 3527907..3529292(-) (pilB) [Escherichia coli strain EC931]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGTCC
AAAGCAGAGTTGCTAACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGGGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCAGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50.131

82.646

0.414
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Acinetobacter baylyi ADP1

39.957

100

0.406
  pilB Haemophilus influenzae Rd KW20

39.451

100

0.406
  pilB Vibrio campbellii strain DS40M4

48.942

81.996

0.401
  pilB Vibrio cholerae strain A1552

47.382

82.863

0.393
  pilB Vibrio parahaemolyticus RIMD 2210633

46.859

82.863

0.388
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilF Neisseria gonorrhoeae MS11

44.619

82.646

0.369
  pilB Acinetobacter baumannii D1279779

44.01

83.297

0.367

Multiple sequence alignment