Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G5C00_RS18210 Genome accession   NZ_CP048934
Coordinates   3782993..3784378 (-) Length   461 a.a.
NCBI ID   WP_001550607.1    Uniprot ID   -
Organism   Escherichia coli strain 190693     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3777993..3789378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5C00_RS18180 (G5C00_18160) mutT 3778442..3778840 (+) 399 WP_001550604.1 8-oxo-dGTP diphosphatase MutT -
  G5C00_RS18185 (G5C00_18165) yacG 3778928..3779125 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  G5C00_RS18190 (G5C00_18170) zapD 3779135..3779878 (-) 744 WP_001194736.1 cell division protein ZapD -
  G5C00_RS18195 (G5C00_18175) coaE 3779878..3780498 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  G5C00_RS24850 - 3780523..3780567 (+) 45 WP_120795372.1 protein YacM -
  G5C00_RS18200 (G5C00_18180) guaC 3780723..3781766 (+) 1044 WP_001217337.1 GMP reductase -
  G5C00_RS18205 (G5C00_18185) hofC 3781801..3783003 (-) 1203 WP_001550605.1 protein transport protein HofC -
  G5C00_RS18210 (G5C00_18190) pilB 3782993..3784378 (-) 1386 WP_001550607.1 type II secretion system protein GspE Machinery gene
  G5C00_RS18215 (G5C00_18195) ppdD 3784388..3784828 (-) 441 WP_001550609.1 prepilin peptidase-dependent pilin -
  G5C00_RS18220 (G5C00_18200) nadC 3785032..3785925 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G5C00_RS18225 (G5C00_18205) ampD 3786013..3786564 (+) 552 WP_001550610.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G5C00_RS18230 (G5C00_18210) ampE 3786561..3787415 (+) 855 WP_001550611.1 beta-lactamase regulator AmpE -
  G5C00_RS18235 (G5C00_18215) aroP 3787458..3788831 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50699.41 Da        Isoelectric Point: 6.6782

>NTDB_id=371844 G5C00_RS18210 WP_001550607.1 3782993..3784378(-) (pilB) [Escherichia coli strain 190693]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCRHCYHGFYGRTALFE
VLPITPIIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=371844 G5C00_RS18210 WP_001550607.1 3782993..3784378(-) (pilB) [Escherichia coli strain 190693]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTGGTCGATGCCCCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGCAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGATTGTTACAGCA
GGTGAGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACAGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACTGCTGATATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGTCTTACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATTATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACCAACTCC
ACCTGCGAAACGCTGGTGCGTTTACAGCAAATGGGCGTTGCTCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGACAGCAAGGGGAGCCAATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCGCTGGCAAGCCCCTGGTTGTAGACACTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTACCCATAACACCGATCATACGCCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACACACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.385

100

0.41
  pilB Vibrio campbellii strain DS40M4

48.446

83.731

0.406
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.667

84.599

0.395
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilB Acinetobacter baumannii D1279779

43.846

84.599

0.371
  pilF Thermus thermophilus HB27

41.278

88.286

0.364

Multiple sequence alignment