Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G3273_RS19265 Genome accession   NZ_CP048647
Coordinates   4020838..4022223 (+) Length   461 a.a.
NCBI ID   WP_001535814.1    Uniprot ID   -
Organism   Escherichia coli strain GW-AmxH19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4015838..4027223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3273_RS19240 (G3273_19240) aroP 4016388..4017758 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  G3273_RS19245 (G3273_19245) ampE 4017801..4018655 (-) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  G3273_RS19250 (G3273_19250) ampD 4018652..4019203 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G3273_RS19255 (G3273_19255) nadC 4019291..4020184 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G3273_RS19260 (G3273_19260) pilA 4020388..4020828 (+) 441 WP_000360913.1 prepilin peptidase-dependent pilin Machinery gene
  G3273_RS19265 (G3273_19265) pilB 4020838..4022223 (+) 1386 WP_001535814.1 type II secretion system protein GspE Machinery gene
  G3273_RS19270 (G3273_19270) hofC 4022213..4023415 (+) 1203 WP_000157243.1 protein transport protein HofC -
  G3273_RS19275 (G3273_19275) guaC 4023450..4024493 (-) 1044 WP_001217338.1 GMP reductase -
  G3273_RS24855 - 4024649..4024693 (-) 45 WP_120795372.1 protein YacM -
  G3273_RS19280 (G3273_19280) coaE 4024718..4025338 (+) 621 WP_001269524.1 dephospho-CoA kinase -
  G3273_RS19285 (G3273_19285) zapD 4025338..4026081 (+) 744 WP_001194731.1 cell division protein ZapD -
  G3273_RS19290 (G3273_19290) yacG 4026091..4026288 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  G3273_RS19295 (G3273_19295) mutT 4026376..4026774 (-) 399 WP_000736044.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50631.34 Da        Isoelectric Point: 6.7134

>NTDB_id=370273 G3273_RS19265 WP_001535814.1 4020838..4022223(+) (pilB) [Escherichia coli strain GW-AmxH19]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLVLVTGPTGSGKTVTLYSALQMLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGKPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=370273 G3273_RS19265 WP_001535814.1 4020838..4022223(+) (pilB) [Escherichia coli strain GW-AmxH19]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGACGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTAGCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAACCAGGCACTGGATGTTAACACGCTTGGAATGCAGCCGTCACAACTGGTGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAATGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATTC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCCGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGAAGCCCATCCATATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

49.74

83.297

0.414
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Acinetobacter baylyi ADP1

39.53

100

0.401
  pilB Vibrio campbellii strain DS40M4

47.532

83.514

0.397
  pilB Vibrio cholerae strain A1552

46.154

84.599

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

45.641

84.599

0.386
  pilB Haemophilus influenzae 86-028NP

44.703

83.948

0.375
  pilB Haemophilus influenzae Rd KW20

43.928

83.948

0.369
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369
  pilB Acinetobacter baumannii D1279779

42.893

85.466

0.367

Multiple sequence alignment