Detailed information    

insolico Bioinformatically predicted

Overview


Name   recA   Type   Machinery gene
Locus tag   DF008_RS02680 Genome accession   NZ_CP029566
Coordinates   579890..580936 (+) Length   348 a.a.
NCBI ID   WP_003428218.1    Uniprot ID   A0A031WFI0
Organism   Clostridioides difficile strain CBA7204     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 547758..628744 579890..580936 within 0


Gene organization within MGE regions


Location: 547758..628744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DF008_RS02535 (DF008_02565) acd 547833..549656 (+) 1824 WP_003438294.1 N-acetylglucosaminidase -
  DF008_RS02540 (DF008_02570) - 549871..554169 (+) 4299 WP_003438301.1 PolC-type DNA polymerase III -
  DF008_RS02545 (DF008_02575) rimP 554373..554834 (+) 462 WP_003438303.1 ribosome maturation factor RimP -
  DF008_RS02550 (DF008_02580) nusA 554864..556018 (+) 1155 WP_003428238.1 transcription termination factor NusA -
  DF008_RS02555 (DF008_02585) rnpM 556025..556306 (+) 282 WP_003419469.1 RNase P modulator RnpM -
  DF008_RS02560 (DF008_02590) - 556296..556607 (+) 312 WP_003419470.1 L7Ae/L30e/S12e/Gadd45 family ribosomal protein -
  DF008_RS02565 (DF008_02595) infB 556629..558569 (+) 1941 WP_003438306.1 translation initiation factor IF-2 -
  DF008_RS02570 (DF008_02600) rbfA 558612..558992 (+) 381 WP_003428234.1 30S ribosome-binding factor RbfA -
  DF008_RS02575 (DF008_02605) - 558973..559650 (+) 678 Protein_504 DHH family phosphoesterase -
  DF008_RS02585 (DF008_02615) tnpB 560299..561417 (+) 1119 WP_032548186.1 IS200/IS605 family element RNA-guided endonuclease TnpB -
  DF008_RS02590 (DF008_02620) - 561535..561840 (+) 306 Protein_506 DHH family phosphoesterase -
  DF008_RS02595 (DF008_02625) truB 561853..562758 (+) 906 WP_003428232.1 tRNA pseudouridine(55) synthase TruB -
  DF008_RS02600 (DF008_02630) - 562889..563818 (+) 930 WP_003438310.1 bifunctional riboflavin kinase/FAD synthetase -
  DF008_RS02610 (DF008_02640) rpsO 563922..564179 (+) 258 WP_003419491.1 30S ribosomal protein S15 -
  DF008_RS02615 (DF008_02645) - 564419..565264 (+) 846 WP_009889099.1 DegV family protein -
  DF008_RS02620 (DF008_02650) pnp 565389..567500 (+) 2112 WP_003438316.1 polyribonucleotide nucleotidyltransferase -
  DF008_RS02625 (DF008_02655) - 567766..568509 (+) 744 WP_003438319.1 polysaccharide deacetylase family protein -
  DF008_RS02630 (DF008_02660) - 568667..569914 (+) 1248 WP_003438321.1 M16 family metallopeptidase -
  DF008_RS02635 (DF008_02665) - 569949..570194 (+) 246 WP_003419503.1 YlmC/YmxH family sporulation protein -
  DF008_RS02640 (DF008_02670) dapG 570204..571427 (+) 1224 WP_003438324.1 aspartate kinase -
  DF008_RS02650 (DF008_02680) tnpB 572216..573334 (+) 1119 WP_159376146.1 IS200/IS605 family element RNA-guided endonuclease TnpB -
  DF008_RS02655 (DF008_02685) - 573521..574150 (+) 630 WP_021363436.1 ClpP family protease -
  DF008_RS02660 (DF008_02690) - 574273..576684 (+) 2412 WP_003438328.1 FtsK/SpoIIIE family DNA translocase -
  DF008_RS02665 (DF008_02695) mnmH 576687..577745 (+) 1059 WP_003433322.1 tRNA 2-selenouridine(34) synthase MnmH -
  DF008_RS02670 (DF008_02700) rimO 577739..579073 (+) 1335 WP_159376147.1 30S ribosomal protein S12 methylthiotransferase RimO -
  DF008_RS02675 (DF008_02705) pgsA 579060..579602 (+) 543 WP_009889106.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  DF008_RS02680 (DF008_02710) recA 579890..580936 (+) 1047 WP_003428218.1 recombinase RecA Machinery gene
  DF008_RS02685 (DF008_02715) rny 581112..582653 (+) 1542 WP_003428216.1 ribonuclease Y -
  DF008_RS02690 (DF008_02720) - 582967..585303 (+) 2337 WP_003438341.1 diguanylate cyclase -
  DF008_RS02695 (DF008_02725) - 585685..587466 (+) 1782 WP_003438344.1 UvrD-helicase domain-containing protein -
  DF008_RS02700 (DF008_02730) lepB 587548..588078 (-) 531 WP_009902411.1 signal peptidase I -
  DF008_RS02705 (DF008_02735) - 588118..588942 (-) 825 WP_003438350.1 undecaprenyl-diphosphate phosphatase -
  DF008_RS02710 (DF008_02740) - 589199..590107 (+) 909 WP_009889110.1 tyrosine-type recombinase/integrase -
  DF008_RS02715 (DF008_02745) purB 590251..591696 (+) 1446 WP_003438353.1 adenylosuccinate lyase -
  DF008_RS02720 (DF008_02750) - 592007..592459 (+) 453 WP_003419561.1 MarR family winged helix-turn-helix transcriptional regulator -
  DF008_RS02725 (DF008_02755) - 592794..593990 (-) 1197 WP_003438357.1 pyridoxal phosphate-dependent aminotransferase -
  DF008_RS02730 (DF008_02760) galT 594375..595325 (+) 951 WP_070538166.1 galactose-1-phosphate uridylyltransferase -
  DF008_RS02735 (DF008_02765) - 595486..596238 (+) 753 WP_003438367.1 exodeoxyribonuclease III -
  DF008_RS02740 (DF008_02770) - 596330..597106 (-) 777 WP_009896237.1 3'-5' exonuclease -
  DF008_RS02745 (DF008_02775) - 597421..599319 (+) 1899 WP_070538168.1 glutamine synthetase -
  DF008_RS02750 (DF008_02780) - 599447..600568 (-) 1122 WP_003428201.1 MGDG synthase family glycosyltransferase -
  DF008_RS02755 (DF008_02785) - 600767..601099 (+) 333 WP_009889116.1 PadR family transcriptional regulator -
  DF008_RS02760 (DF008_02790) - 601086..602144 (+) 1059 WP_003438377.1 DUF1700 domain-containing protein -
  DF008_RS02765 (DF008_02795) - 602137..603207 (+) 1071 WP_011861183.1 DUF4097 family beta strand repeat-containing protein -
  DF008_RS02770 (DF008_02800) - 603309..603845 (+) 537 WP_075052540.1 hypothetical protein -
  DF008_RS02775 (DF008_02805) - 603961..604668 (+) 708 WP_016729592.1 lantibiotic protection ABC transporter ATP-binding protein -
  DF008_RS02780 (DF008_02810) - 604670..605386 (+) 717 WP_016729591.1 lantibiotic immunity ABC transporter MutE/EpiE family permease subunit -
  DF008_RS02785 (DF008_02815) - 605388..606146 (+) 759 WP_159376148.1 lantibiotic immunity ABC transporter MutG family permease subunit -
  DF008_RS02790 (DF008_02820) - 606281..607669 (+) 1389 WP_159376149.1 two-component system sensor histidine kinase -
  DF008_RS02795 (DF008_02825) - 607745..608566 (-) 822 WP_003428191.1 pyridoxamine kinase -
  DF008_RS02800 (DF008_02830) - 608817..609971 (+) 1155 WP_009892989.1 amidase domain-containing protein -
  DF008_RS02805 (DF008_02835) - 610154..610354 (+) 201 WP_003419624.1 cold-shock protein -
  DF008_RS02810 (DF008_02840) - 610775..611203 (+) 429 WP_009889129.1 CBS domain-containing protein -
  DF008_RS02815 (DF008_02845) - 611346..612101 (-) 756 WP_009889131.1 peptidylprolyl isomerase -
  DF008_RS02825 (DF008_02855) - 612802..613308 (-) 507 WP_011861186.1 ImmA/IrrE family metallo-endopeptidase -
  DF008_RS02830 (DF008_02860) - 613390..613710 (-) 321 WP_003428185.1 XRE family transcriptional regulator -
  DF008_RS02835 (DF008_02865) - 613879..614076 (+) 198 WP_003419641.1 helix-turn-helix transcriptional regulator -
  DF008_RS02840 (DF008_02870) - 614180..614620 (+) 441 WP_003428183.1 hypothetical protein -
  DF008_RS02845 (DF008_02875) - 614869..615312 (+) 444 WP_159376150.1 phage tail terminator family protein -
  DF008_RS02850 (DF008_02880) - 615317..616381 (+) 1065 WP_003438414.1 phage tail sheath subtilisin-like domain-containing protein -
  DF008_RS02855 (DF008_02885) - 616395..616823 (+) 429 WP_003419646.1 phage tail tube protein -
  DF008_RS02860 (DF008_02890) - 616949..617395 (+) 447 WP_003419647.1 phage tail assembly chaperone -
  DF008_RS02865 (DF008_02895) - 617422..617589 (+) 168 WP_003419648.1 hypothetical protein -
  DF008_RS02870 (DF008_02900) - 617589..620042 (+) 2454 WP_159376151.1 hypothetical protein -
  DF008_RS02875 (DF008_02905) - 620042..620464 (+) 423 WP_003428168.1 phage baseplate protein -
  DF008_RS02880 (DF008_02910) - 620746..622275 (+) 1530 WP_009902435.1 NlpC/P60 family protein -
  DF008_RS02885 (DF008_02915) - 622291..622617 (+) 327 WP_003438447.1 DUF2577 domain-containing protein -
  DF008_RS02890 (DF008_02920) - 622617..623045 (+) 429 WP_003438449.1 DUF2634 domain-containing protein -
  DF008_RS02895 (DF008_02925) - 623038..624090 (+) 1053 WP_003438450.1 baseplate J/gp47 family protein -
  DF008_RS02900 (DF008_02930) - 624087..624785 (+) 699 WP_003428156.1 putative phage tail protein -
  DF008_RS02905 (DF008_02935) - 624786..625769 (+) 984 WP_021366456.1 phage tail protein -

Sequence


Protein


Download         Length: 348 a.a.        Molecular weight: 37377.68 Da        Isoelectric Point: 4.8236

>NTDB_id=293667 DF008_RS02680 WP_003428218.1 579890..580936(+) (recA) [Clostridioides difficile strain CBA7204]
MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTGAIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSC
VASAQKDGGIAAFIDAEHALDPVYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKFYSSVRLDVRKIDTIKQGDKVIG
SRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLLDIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEI
DEKVRAFYNLNEEHEESGTSVSKEIVEE

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=293667 DF008_RS02680 WP_003428218.1 579890..580936(+) (recA) [Clostridioides difficile strain CBA7204]
ATGAGTGTAGATCAAGAAAAATTAAAAGCGTTGAATGAAGCTTTAGGTAAAATTGAAAAAGATTTTGGTAAAGGTTCAGT
AATGAAATTGGGAGAAGCAACATCTATGTCTATAGATGTTATATCAACAGGAGCGATTGGTTTAGACATAGCTATTGGTA
TAGGAGGTCTACCTAGAGGGAGAATAGTTGAAGTATATGGTCCAGAATCTTCTGGTAAGACTACTGTTGCGCTTAGTTGT
GTAGCATCAGCTCAAAAAGATGGAGGAATAGCTGCATTTATAGATGCAGAACACGCACTTGACCCAGTATATGCAAAAGC
TTTGGGTGTGGATGTTGATAACCTAATAATATCTCAACCAGATACAGGTGAACAGGCTTTAGAGATAGCAGAGGCATTGA
TAAGAAGTGGAGCGATAGATATAATAGTAATAGACTCAGTAGCAGCATTAGTTCCAAAGGCTGAAATAGATGGAGATATG
GGTGATTCTCATGTAGGTTTACAAGCTAGACTTATGTCACAAGCACTTAGAAAGTTAACTGGTTCAATTAAAAAATCAAA
TTGTGTTGCTATATTTATAAACCAGTTAAGAGAGAAAGTAGGAATAATGTTTGGAAACCCAGAAACTACTACTGGAGGAC
GTGCACTAAAATTCTATTCATCAGTTAGATTGGATGTTAGAAAAATAGATACAATAAAACAAGGTGATAAAGTTATAGGT
AGTAGAACTAGAGTTAAAGTTGTTAAAAACAAAGTAGCACCACCATTTAAGCAAGCTGAATTTGATATAATGTATGGAGA
AGGGATTTCAAAAATTGGAGACCTTTTAGATATAGCTGCTGATGTAGATATAGTTAAAAAATCAGGTTCATGGTATAGTT
ACAATGATACTAAACTTGGACAAGGAAGAGAAAATGTTAAAAAATTCTTGGAAGATAATTTAGATTTAACTACTGAAATA
GATGAGAAAGTTAGAGCATTTTACAATTTAAATGAAGAACATGAAGAATCAGGTACTTCAGTATCAAAAGAAATTGTAGA
AGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A031WFI0

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Streptococcus mutans UA159

66.762

100

0.67

  recA Streptococcus mitis SK321

67.456

97.126

0.655

  recA Streptococcus pneumoniae Rx1

65.507

99.138

0.649

  recA Streptococcus pneumoniae R6

65.507

99.138

0.649

  recA Streptococcus pneumoniae D39

65.507

99.138

0.649

  recA Streptococcus pneumoniae TIGR4

65.507

99.138

0.649

  recA Streptococcus mitis NCTC 12261

68.085

94.54

0.644

  recA Bacillus subtilis subsp. subtilis str. 168

69.565

92.529

0.644

  recA Lactococcus lactis subsp. cremoris KW2

65.569

95.977

0.629

  recA Streptococcus pyogenes NZ131

66.972

93.966

0.629

  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

65.758

94.828

0.624

  recA Latilactobacillus sakei subsp. sakei 23K

64.955

95.115

0.618

  recA Neisseria gonorrhoeae MS11

66.049

93.103

0.615

  recA Neisseria gonorrhoeae MS11

66.049

93.103

0.615

  recA Neisseria gonorrhoeae strain FA1090

66.049

93.103

0.615

  recA Acinetobacter baylyi ADP1

64.396

92.816

0.598

  recA Pseudomonas stutzeri DSM 10701

63.889

93.103

0.595

  recA Helicobacter pylori 26695

61.976

95.977

0.595

  recA Helicobacter pylori strain NCTC11637

61.976

95.977

0.595

  recA Acinetobacter baumannii D1279779

63.777

92.816

0.592

  recA Vibrio cholerae O1 biovar El Tor strain E7946

62.048

95.402

0.592

  recA Vibrio cholerae strain A1552

62.048

95.402

0.592

  recA Glaesserella parasuis strain SC1401

64.174

92.241

0.592

  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

63.272

93.103

0.589

  recA Ralstonia pseudosolanacearum GMI1000

66.558

88.506

0.589

  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

60.681

92.816

0.563


Multiple sequence alignment