Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CDC45_RS15975 Genome accession   NZ_CP021762
Coordinates   3352648..3354204 (+) Length   518 a.a.
NCBI ID   WP_028853770.1    Uniprot ID   A0ABX7ZQ67
Organism   Ralstonia pseudosolanacearum strain RS 476     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3347648..3359204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDC45_RS15960 (CDC45_15900) gspM 3348729..3349307 (+) 579 WP_011003003.1 type II secretion system protein GspM -
  CDC45_RS15965 (CDC45_15905) - 3349311..3350162 (+) 852 WP_011003004.1 type II secretion system protein N -
  CDC45_RS15970 (CDC45_15910) gspD 3350196..3352613 (+) 2418 WP_011003005.1 type II secretion system secretin GspD -
  CDC45_RS15975 (CDC45_15915) pilF 3352648..3354204 (+) 1557 WP_028853770.1 type II secretion system ATPase GspE Machinery gene
  CDC45_RS15980 (CDC45_15920) gspF 3354206..3355417 (+) 1212 WP_011003007.1 type II secretion system inner membrane protein GspF -
  CDC45_RS15985 (CDC45_15925) - 3355725..3357008 (+) 1284 WP_011003008.1 hypothetical protein -
  CDC45_RS15990 (CDC45_15930) - 3357084..3358370 (+) 1287 WP_020371584.1 hypothetical protein -
  CDC45_RS27270 - 3358405..3358506 (+) 102 Protein_3131 preprotein translocase subunit SecF -

Sequence

Protein


Download         Length: 518 a.a.        Molecular weight: 55861.86 Da        Isoelectric Point: 5.5004

>NTDB_id=195740 CDC45_RS15975 WP_028853770.1 3352648..3354204(+) (pilF) [Ralstonia pseudosolanacearum strain RS 476]
MATQASSTDARTLEPPSPSAVRLVPYVFARDARLLVARQDVDSLEVWVCPETSRAALAELARVFGALRLVSLDAAALSAA
TQTAYNAQDGSAAQVVGEVEGEVDLSRLMQDIPAVEDLLESEDDAPIIRMINALLTQAAREGASDIHIEPFENASVVRFR
VDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLDKEAGRLDL
GKLGMGAGTLGRFDRLINQPHGIVLVTGPTGSGKTTTLYAALSRLDASSTNIMTVEDPIEYDLDGIGQTQVNAKIDMTFA
KALRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLATLHTNDSASAVTRLIDMGIEPFLLSSSLLGVLAQRLVRRL
CVHCRREEVLELTPTEIEAVAGTPPADGTAAAVPTRRSVWHHVGCEHCGQSGYQGRTGVYELLTVTPEIQTMIHRQAPES
EIKALAIGQGMHTMRADAQRWLDAGATSLEEVLRVTRD

Nucleotide


Download         Length: 1557 bp        

>NTDB_id=195740 CDC45_RS15975 WP_028853770.1 3352648..3354204(+) (pilF) [Ralstonia pseudosolanacearum strain RS 476]
ATGGCAACGCAAGCTTCTTCCACTGACGCGCGCACGCTCGAGCCGCCGTCGCCCTCGGCGGTGCGGCTGGTGCCGTATGT
GTTCGCGCGCGACGCCAGGCTGCTGGTGGCGCGGCAGGATGTCGACTCGCTCGAGGTGTGGGTCTGCCCCGAGACCTCGC
GCGCCGCGCTGGCCGAGCTGGCCCGCGTGTTCGGCGCGCTGCGCCTGGTGAGCCTGGACGCCGCCGCGCTCTCCGCCGCC
ACGCAGACGGCCTACAACGCCCAGGACGGCAGCGCGGCCCAGGTGGTCGGCGAGGTCGAGGGCGAGGTGGACCTGTCGCG
CCTGATGCAGGACATCCCGGCCGTGGAAGACCTGCTCGAATCGGAAGACGACGCGCCGATCATCCGCATGATCAACGCGC
TGCTCACGCAGGCCGCACGCGAGGGCGCCTCGGACATCCACATCGAGCCGTTCGAGAACGCCTCGGTGGTGCGCTTCCGC
GTGGACGGCACGCTGCGCGACGTGGTGCGCCCGAAGAAGGCGCTGCACGGCGCGCTGATCTCGCGCATCAAGATCATGGC
GCAGCTCGACATCGCCGAGAAACGCCTGCCGCAGGACGGCCGCATCACGCTGCGCGTGGGCGGGCGACCGGTGGACGTGC
GTGTCTCCACCCTGCCGACCGGCCACGGCGAGCGCGCGGTGCTGCGCCTGCTCGACAAGGAAGCCGGCCGGCTTGACCTC
GGCAAGCTCGGCATGGGCGCCGGCACGCTGGGGCGCTTCGACCGCCTGATCAACCAGCCGCACGGCATCGTGCTCGTCAC
CGGCCCGACCGGCTCGGGCAAGACCACCACGCTGTATGCCGCGCTGTCGCGGCTCGATGCCAGCAGCACCAACATCATGA
CGGTGGAGGACCCGATCGAGTACGACCTGGACGGTATCGGCCAGACCCAGGTCAACGCGAAGATCGACATGACCTTCGCC
AAGGCCCTGCGCGCCATCCTGCGGCAGGACCCGGACGTGGTGATGATCGGCGAGATCCGCGACCTGGAGACCGCGCAGAT
CGCGGTGCAGGCCTCGCTGACCGGCCACCTGGTGCTGGCGACGCTGCACACCAACGATTCGGCCTCGGCGGTCACGCGGC
TGATCGACATGGGGATCGAGCCGTTCCTGCTGTCGTCGTCGCTGCTGGGCGTGCTGGCGCAGCGGCTGGTGCGGCGCCTG
TGCGTGCACTGCCGCCGCGAGGAAGTGCTGGAGCTCACCCCCACCGAGATCGAGGCCGTGGCCGGCACGCCGCCCGCCGA
CGGCACCGCCGCCGCCGTGCCGACGCGCCGCTCGGTGTGGCACCACGTCGGCTGCGAGCACTGCGGCCAATCGGGCTACC
AGGGCCGGACCGGCGTGTACGAGCTGCTGACGGTGACGCCCGAGATCCAGACCATGATCCACCGCCAGGCGCCCGAGTCC
GAGATCAAGGCGCTCGCCATCGGCCAGGGCATGCATACCATGCGCGCGGATGCGCAGCGGTGGCTGGACGCGGGCGCGAC
GTCGCTGGAAGAGGTGCTGCGCGTGACGCGCGATTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(133-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

42.744

97.104

0.415
  pilB Vibrio cholerae strain A1552

45.657

86.68

0.396
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.689

99.228

0.394
  pilB Vibrio campbellii strain DS40M4

44.835

87.838

0.394
  pilB/pilB1 Synechocystis sp. PCC 6803

44.676

83.398

0.373
  pilB Vibrio parahaemolyticus RIMD 2210633

46.324

78.764

0.365

Multiple sequence alignment