Detailed information    

insolico Bioinformatically predicted

Overview


Name   recA   Type   Machinery gene
Locus tag   Q5O_RS20480 Genome accession   NZ_CP016212
Coordinates   4498870..4499937 (-) Length   355 a.a.
NCBI ID   WP_003252365.1    Uniprot ID   I7B6K4
Organism   Pseudomonas putida JB     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 4497291..4544488 4498870..4499937 within 0


Gene organization within MGE regions


Location: 4497291..4544488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5O_RS20475 (Q5O_20475) recX 4498378..4498848 (-) 471 WP_019436651.1 recombination regulator RecX -
  Q5O_RS20480 (Q5O_20480) recA 4498870..4499937 (-) 1068 WP_003252365.1 recombinase RecA Machinery gene
  Q5O_RS20485 (Q5O_20485) - 4500041..4500523 (-) 483 WP_010952695.1 CinA family protein -
  Q5O_RS20490 (Q5O_20490) - 4500579..4501646 (-) 1068 WP_014592125.1 hypothetical protein -
  Q5O_RS20495 (Q5O_20495) - 4502422..4503417 (+) 996 WP_070384245.1 DUF4238 domain-containing protein -
  Q5O_RS20500 (Q5O_20500) - 4503419..4504609 (+) 1191 WP_070384246.1 hypothetical protein -
  Q5O_RS20505 (Q5O_20505) - 4504941..4505735 (-) 795 WP_070384247.1 DNA adenine methylase -
  Q5O_RS20510 (Q5O_20510) - 4505889..4506443 (-) 555 WP_070384248.1 lysis system i-spanin subunit Rz -
  Q5O_RS20515 (Q5O_20515) - 4506443..4507012 (-) 570 WP_070384249.1 glycoside hydrolase family 19 protein -
  Q5O_RS20520 (Q5O_20520) - 4507051..4508094 (-) 1044 WP_070384250.1 phage late control D family protein -
  Q5O_RS20525 (Q5O_20525) - 4508154..4508360 (-) 207 WP_070384251.1 tail protein X -
  Q5O_RS20530 (Q5O_20530) - 4508335..4509180 (-) 846 WP_070384252.1 phage tail protein -
  Q5O_RS20535 (Q5O_20535) - 4509190..4513089 (-) 3900 WP_070384253.1 phage tail tape measure protein -
  Q5O_RS20540 (Q5O_20540) - 4513221..4513517 (-) 297 WP_070384254.1 phage tail assembly protein -
  Q5O_RS20545 (Q5O_20545) - 4513566..4514075 (-) 510 WP_070384255.1 phage major tail tube protein -
  Q5O_RS20550 (Q5O_20550) - 4514088..4515263 (-) 1176 WP_070384256.1 phage tail sheath family protein -
  Q5O_RS20555 (Q5O_20555) - 4515328..4515651 (-) 324 WP_081343634.1 hypothetical protein -
  Q5O_RS20560 (Q5O_20560) - 4515716..4516180 (-) 465 WP_070384257.1 hypothetical protein -
  Q5O_RS20565 (Q5O_20565) - 4516188..4516928 (-) 741 WP_070384258.1 hypothetical protein -
  Q5O_RS20570 (Q5O_20570) - 4516932..4517798 (-) 867 WP_081343635.1 phage tail protein -
  Q5O_RS20575 (Q5O_20575) - 4517795..4518517 (-) 723 WP_070384259.1 phage tail protein I -
  Q5O_RS20580 (Q5O_20580) - 4518514..4519494 (-) 981 WP_070384260.1 baseplate assembly protein -
  Q5O_RS20585 (Q5O_20585) - 4519491..4519817 (-) 327 WP_070384261.1 GPW/gp25 family protein -
  Q5O_RS20590 (Q5O_20590) - 4519820..4520098 (-) 279 WP_070384262.1 hypothetical protein -
  Q5O_RS20595 (Q5O_20595) - 4520142..4520744 (-) 603 WP_070384263.1 phage baseplate assembly protein V -
  Q5O_RS20600 (Q5O_20600) - 4520741..4521271 (-) 531 WP_070384264.1 hypothetical protein -
  Q5O_RS20605 (Q5O_20605) - 4521264..4521932 (-) 669 WP_070384265.1 hypothetical protein -
  Q5O_RS20610 (Q5O_20610) - 4521929..4522252 (-) 324 WP_070384266.1 head-tail joining protein -
  Q5O_RS20615 (Q5O_20615) - 4522255..4522437 (-) 183 WP_070384267.1 hypothetical protein -
  Q5O_RS20620 (Q5O_20620) - 4522439..4523467 (-) 1029 WP_070384268.1 major capsid protein -
  Q5O_RS20625 (Q5O_20625) - 4523479..4523829 (-) 351 WP_070384269.1 head decoration protein -
  Q5O_RS20630 (Q5O_20630) - 4523843..4525063 (-) 1221 WP_070384270.1 S49 family peptidase -
  Q5O_RS20635 (Q5O_20635) - 4525066..4526673 (-) 1608 WP_070384271.1 phage portal protein -
  Q5O_RS20640 (Q5O_20640) - 4526676..4526891 (-) 216 WP_070384272.1 hypothetical protein -
  Q5O_RS20645 (Q5O_20645) - 4526904..4528790 (-) 1887 WP_172830939.1 phage terminase large subunit family protein -
  Q5O_RS20650 (Q5O_20650) - 4528834..4529355 (-) 522 WP_070384274.1 terminase small subunit -
  Q5O_RS20655 (Q5O_20655) - 4529498..4529845 (-) 348 WP_070384275.1 phage holin family protein -
  Q5O_RS20660 (Q5O_20660) - 4530050..4530601 (-) 552 WP_081343636.1 hypothetical protein -
  Q5O_RS27780 - 4530591..4530929 (-) 339 WP_155766717.1 hypothetical protein -
  Q5O_RS20665 (Q5O_20665) - 4530926..4533691 (-) 2766 WP_070384276.1 bifunctional DNA primase/polymerase -
  Q5O_RS20670 (Q5O_20670) - 4533688..4533954 (-) 267 WP_420492588.1 TraR/DksA C4-type zinc finger protein -
  Q5O_RS20675 (Q5O_20675) - 4533884..4534372 (-) 489 WP_070384278.1 phage regulatory CII family protein -
  Q5O_RS20680 (Q5O_20680) - 4534601..4534795 (-) 195 WP_070384279.1 helix-turn-helix domain-containing protein -
  Q5O_RS20685 (Q5O_20685) - 4534846..4535553 (+) 708 WP_257785624.1 LexA family protein -
  Q5O_RS20690 (Q5O_20690) - 4535808..4536317 (+) 510 WP_070384280.1 hypothetical protein -
  Q5O_RS20695 (Q5O_20695) - 4536314..4536553 (+) 240 WP_060494954.1 pyocin activator PrtN family protein -
  Q5O_RS20700 (Q5O_20700) - 4536644..4537162 (+) 519 WP_070384281.1 hypothetical protein -
  Q5O_RS27375 - 4537159..4537413 (+) 255 WP_081343637.1 AAA family ATPase -
  Q5O_RS28290 - 4537410..4537538 (+) 129 WP_257785614.1 hypothetical protein -
  Q5O_RS20705 (Q5O_20705) - 4537535..4537819 (+) 285 WP_155766718.1 hypothetical protein -
  Q5O_RS20710 (Q5O_20710) - 4538009..4538194 (+) 186 WP_070384283.1 hypothetical protein -
  Q5O_RS20715 (Q5O_20715) - 4538191..4538913 (+) 723 WP_070384284.1 hypothetical protein -
  Q5O_RS20720 (Q5O_20720) - 4538910..4539104 (+) 195 WP_060495023.1 hypothetical protein -
  Q5O_RS20725 (Q5O_20725) - 4539074..4540321 (+) 1248 WP_070384285.1 site-specific integrase -
  Q5O_RS20730 (Q5O_20730) - 4540566..4541237 (-) 672 WP_070384286.1 hypothetical protein -
  Q5O_RS27785 - 4541258..4541938 (-) 681 WP_155766719.1 hypothetical protein -
  Q5O_RS20740 (Q5O_20740) - 4541935..4542468 (-) 534 WP_070384288.1 hypothetical protein -

Sequence


Protein


Download         Length: 355 a.a.        Molecular weight: 37544.89 Da        Isoelectric Point: 5.3228

>NTDB_id=186217 Q5O_RS20480 WP_003252365.1 4498870..4499937(-) (recA) [Pseudomonas putida JB]
MDDNKKRALAAALGQIERQFGKGAVMRMGDHERQGIPAISTGSLGLDIALGIGGLPKGRIVEIYGPESSGKTTLTLSVIA
EAQKNGATCAFVDAEHALDPEYAGKLGVNVDDLLVSQPDTGEQALEITDMLVRSNAVDVIIVDSVAALVPKAEIEGEMGD
MHVGLQARLMSQALRKITGNIKNANCLVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRTGAVKEGDEVVGSE
TRVKIVKNKVSPPFRQAEFQILYGKGIYRNGEIIDLGVSQGLVEKSGAWYAYQGNKIGQGKANAAKYLAENPAIGAEIEK
QIREKLLKAGAAAEAGKAAAAEADADDMADADAGY

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=186217 Q5O_RS20480 WP_003252365.1 4498870..4499937(-) (recA) [Pseudomonas putida JB]
ATGGACGACAACAAGAAGCGCGCCTTGGCTGCGGCCCTGGGTCAGATCGAACGCCAATTCGGTAAAGGCGCGGTCATGCG
CATGGGTGACCATGAGCGTCAAGGCATTCCGGCCATCTCCACCGGCTCGCTGGGCTTGGATATCGCACTGGGCATCGGCG
GTCTGCCAAAAGGCCGTATCGTCGAGATCTACGGCCCGGAATCGTCGGGTAAGACCACGCTCACCCTGTCTGTCATCGCT
GAAGCGCAGAAGAACGGTGCTACCTGCGCCTTCGTCGACGCCGAACATGCCCTTGACCCTGAGTACGCCGGCAAGCTGGG
CGTCAACGTCGATGACCTGCTGGTTTCGCAGCCGGACACCGGTGAGCAGGCCCTTGAAATCACCGACATGCTGGTGCGTT
CCAACGCGGTCGACGTGATCATTGTCGACTCCGTTGCCGCGCTGGTACCGAAGGCCGAGATCGAAGGCGAGATGGGTGAC
ATGCATGTGGGTCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGCAAGATCACCGGTAACATCAAGAACGCGAACTGCCT
GGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGCCCGGAAACCACTACCGGTGGTAACGCCC
TGAAGTTCTATGCTTCGGTCCGTCTGGACATCCGCCGTACCGGCGCGGTCAAGGAAGGCGACGAAGTGGTCGGCAGCGAA
ACCCGCGTCAAGATCGTCAAGAACAAGGTCTCGCCTCCGTTCCGTCAGGCTGAGTTCCAGATTCTTTACGGGAAAGGTAT
CTACCGTAACGGCGAGATCATTGATCTGGGGGTATCTCAGGGCTTGGTCGAGAAGTCCGGCGCCTGGTACGCCTACCAAG
GCAACAAGATCGGTCAAGGCAAAGCCAACGCTGCCAAGTACCTGGCTGAGAACCCGGCTATTGGTGCCGAGATCGAGAAG
CAGATTCGTGAAAAGTTGCTGAAAGCGGGTGCTGCTGCTGAAGCCGGCAAAGCTGCTGCTGCCGAAGCTGATGCCGATGA
CATGGCCGACGCTGACGCCGGTTATTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB I7B6K4

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Pseudomonas stutzeri DSM 10701

90.462

97.465

0.882

  recA Acinetobacter baylyi ADP1

76.758

92.113

0.707

  recA Acinetobacter baumannii D1279779

74.924

93.239

0.699

  recA Vibrio cholerae strain A1552

74.312

92.113

0.685

  recA Vibrio cholerae O1 biovar El Tor strain E7946

74.312

92.113

0.685

  recA Glaesserella parasuis strain SC1401

68.571

98.592

0.676

  recA Ralstonia pseudosolanacearum GMI1000

71.045

94.366

0.67

  recA Neisseria gonorrhoeae MS11

71.296

91.268

0.651

  recA Neisseria gonorrhoeae MS11

71.296

91.268

0.651

  recA Neisseria gonorrhoeae strain FA1090

71.296

91.268

0.651

  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

64.526

92.113

0.594

  recA Helicobacter pylori strain NCTC11637

63.303

92.113

0.583

  recA Helicobacter pylori 26695

62.691

92.113

0.577

  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

61.631

93.239

0.575

  recA Bacillus subtilis subsp. subtilis str. 168

62.305

90.423

0.563

  recA Streptococcus pyogenes NZ131

55.742

100

0.561

  recA Streptococcus mutans UA159

55.932

99.718

0.558

  recA Streptococcus mitis NCTC 12261

56.484

97.746

0.552

  recA Streptococcus mitis SK321

56.484

97.746

0.552

  recA Streptococcus pneumoniae Rx1

58.896

91.831

0.541

  recA Streptococcus pneumoniae D39

58.896

91.831

0.541

  recA Streptococcus pneumoniae R6

58.896

91.831

0.541

  recA Streptococcus pneumoniae TIGR4

58.896

91.831

0.541

  recA Latilactobacillus sakei subsp. sakei 23K

59.19

90.423

0.535

  recA Lactococcus lactis subsp. cremoris KW2

57.276

90.986

0.521

  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

55.828

91.831

0.513


Multiple sequence alignment