Detailed information    

insolico Bioinformatically predicted

Overview


Name   recA   Type   Machinery gene
Locus tag   CDIF28670_RS07210 Genome accession   NZ_CP012312
Coordinates   1448125..1449171 (+) Length   348 a.a.
NCBI ID   WP_003428218.1    Uniprot ID   A0A031WFI0
Organism   Clostridioides difficile strain DSM 28670     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1414489..1486054 1448125..1449171 within 0


Gene organization within MGE regions


Location: 1414489..1486054
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDIF28670_RS07055 (CDIF28670_01401) - 1414537..1414935 (+) 399 WP_003428254.1 spore germination protein GerW family protein -
  CDIF28670_RS07060 (CDIF28670_01402) - 1415183..1415623 (+) 441 WP_003419459.1 MarR family winged helix-turn-helix transcriptional regulator -
  CDIF28670_RS07065 (CDIF28670_01403) - 1415639..1417090 (+) 1452 WP_118712284.1 leucyl aminopeptidase -
  CDIF28670_RS07070 (CDIF28670_01404) - 1417181..1417849 (-) 669 WP_003428250.1 YwaF family protein -
  CDIF28670_RS07075 (CDIF28670_01405) - 1418118..1418258 (+) 141 WP_003419462.1 hypothetical protein -
  CDIF28670_RS07080 (CDIF28670_01406) - 1418433..1418753 (-) 321 WP_003428248.1 MazG-like family protein -
  CDIF28670_RS07085 (CDIF28670_01407) - 1418873..1419610 (-) 738 WP_003428245.1 NAD-dependent protein deacylase -
  CDIF28670_RS07090 (CDIF28670_01408) acd 1419825..1421648 (+) 1824 WP_070117384.1 N-acetylglucosaminidase -
  CDIF28670_RS07095 (CDIF28670_01409) - 1421863..1426161 (+) 4299 WP_003428241.1 PolC-type DNA polymerase III -
  CDIF28670_RS07100 (CDIF28670_01410) rimP 1426365..1426826 (+) 462 WP_003428240.1 ribosome maturation factor RimP -
  CDIF28670_RS07105 (CDIF28670_01411) nusA 1426856..1428010 (+) 1155 WP_003428238.1 transcription termination factor NusA -
  CDIF28670_RS07110 (CDIF28670_01412) rnpM 1428017..1428298 (+) 282 WP_003419469.1 RNase P modulator RnpM -
  CDIF28670_RS07115 (CDIF28670_01413) - 1428288..1428599 (+) 312 WP_103929441.1 L7Ae/L30e/S12e/Gadd45 family ribosomal protein -
  CDIF28670_RS07120 (CDIF28670_01414) infB 1428621..1430561 (+) 1941 WP_003428235.1 translation initiation factor IF-2 -
  CDIF28670_RS07125 (CDIF28670_01415) rbfA 1430604..1430984 (+) 381 WP_003428234.1 30S ribosome-binding factor RbfA -
  CDIF28670_RS07130 (CDIF28670_01416) - 1430965..1431948 (+) 984 WP_003428233.1 DHH family phosphoesterase -
  CDIF28670_RS07135 (CDIF28670_01417) truB 1431961..1432866 (+) 906 WP_003428232.1 tRNA pseudouridine(55) synthase TruB -
  CDIF28670_RS07140 (CDIF28670_01418) - 1432997..1433926 (+) 930 WP_003428231.1 bifunctional riboflavin kinase/FAD synthetase -
  CDIF28670_RS07150 (CDIF28670_01419) rpsO 1434030..1434287 (+) 258 WP_003419491.1 30S ribosomal protein S15 -
  CDIF28670_RS07155 (CDIF28670_01420) - 1434527..1435372 (+) 846 WP_022616925.1 DegV family protein -
  CDIF28670_RS07160 (CDIF28670_01421) pnp 1435497..1437608 (+) 2112 WP_022616926.1 polyribonucleotide nucleotidyltransferase -
  CDIF28670_RS07165 (CDIF28670_01422) - 1437874..1438617 (+) 744 WP_003428228.1 polysaccharide deacetylase family protein -
  CDIF28670_RS07170 (CDIF28670_01423) - 1438775..1440022 (+) 1248 WP_003428227.1 M16 family metallopeptidase -
  CDIF28670_RS07175 (CDIF28670_01424) - 1440057..1440302 (+) 246 WP_003419503.1 YlmC/YmxH family sporulation protein -
  CDIF28670_RS07180 (CDIF28670_01425) dapG 1440312..1441535 (+) 1224 WP_003428226.1 aspartate kinase -
  CDIF28670_RS07185 (CDIF28670_01426) - 1441648..1442382 (+) 735 WP_003428224.1 ClpP family protease -
  CDIF28670_RS07190 (CDIF28670_01427) - 1442505..1444919 (+) 2415 WP_074097385.1 FtsK/SpoIIIE family DNA translocase -
  CDIF28670_RS07195 (CDIF28670_01428) mnmH 1444922..1445980 (+) 1059 WP_003428222.1 tRNA 2-selenouridine(34) synthase MnmH -
  CDIF28670_RS07200 (CDIF28670_01429) rimO 1445974..1447308 (+) 1335 WP_118712286.1 30S ribosomal protein S12 methylthiotransferase RimO -
  CDIF28670_RS07205 (CDIF28670_01430) pgsA 1447295..1447837 (+) 543 WP_021387802.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  CDIF28670_RS07210 (CDIF28670_01431) recA 1448125..1449171 (+) 1047 WP_003428218.1 recombinase RecA Machinery gene
  CDIF28670_RS07215 (CDIF28670_01432) rny 1449347..1450888 (+) 1542 WP_003428216.1 ribonuclease Y -
  CDIF28670_RS07220 (CDIF28670_01433) - 1451523..1453304 (+) 1782 WP_003428215.1 UvrD-helicase domain-containing protein -
  CDIF28670_RS07225 (CDIF28670_01434) lepB 1453386..1453916 (-) 531 WP_021387803.1 signal peptidase I -
  CDIF28670_RS07230 (CDIF28670_01435) - 1453956..1454780 (-) 825 WP_003438350.1 undecaprenyl-diphosphate phosphatase -
  CDIF28670_RS07235 (CDIF28670_01436) - 1455037..1455945 (+) 909 WP_009889110.1 tyrosine-type recombinase/integrase -
  CDIF28670_RS07240 (CDIF28670_01437) purB 1456089..1457534 (+) 1446 WP_022616928.1 adenylosuccinate lyase -
  CDIF28670_RS07245 (CDIF28670_01438) - 1457844..1458296 (+) 453 WP_003428210.1 MarR family winged helix-turn-helix transcriptional regulator -
  CDIF28670_RS07250 (CDIF28670_01439) - 1458700..1460277 (+) 1578 WP_022616929.1 PTS transporter subunit EIIC -
  CDIF28670_RS07255 (CDIF28670_01440) - 1460425..1461177 (+) 753 WP_022616930.1 MurR/RpiR family transcriptional regulator -
  CDIF28670_RS07260 (CDIF28670_01441) - 1461310..1462677 (+) 1368 WP_022616931.1 6-phospho-alpha-glucosidase -
  CDIF28670_RS07265 (CDIF28670_01442) - 1462768..1463964 (-) 1197 WP_118712288.1 pyridoxal phosphate-dependent aminotransferase -
  CDIF28670_RS07270 (CDIF28670_01443) galT 1464349..1465299 (+) 951 WP_003428205.1 galactose-1-phosphate uridylyltransferase -
  CDIF28670_RS07275 (CDIF28670_01444) - 1465452..1466204 (+) 753 WP_003428204.1 exodeoxyribonuclease III -
  CDIF28670_RS07280 (CDIF28670_01445) - 1466296..1467072 (-) 777 WP_009896237.1 3'-5' exonuclease -
  CDIF28670_RS07285 (CDIF28670_01446) - 1467387..1469285 (+) 1899 WP_003428202.1 glutamine synthetase -
  CDIF28670_RS07290 (CDIF28670_01447) - 1469413..1470534 (-) 1122 WP_003428201.1 MGDG synthase family glycosyltransferase -
  CDIF28670_RS07295 (CDIF28670_01448) - 1470733..1471065 (+) 333 WP_009889116.1 PadR family transcriptional regulator -
  CDIF28670_RS07300 (CDIF28670_01449) - 1471052..1472110 (+) 1059 WP_003428198.1 DUF1700 domain-containing protein -
  CDIF28670_RS07305 (CDIF28670_01450) - 1472103..1473173 (+) 1071 WP_003428197.1 DUF4097 family beta strand repeat-containing protein -
  CDIF28670_RS07310 (CDIF28670_01451) - 1473275..1473811 (+) 537 WP_003428196.1 lipoprotein -
  CDIF28670_RS07315 (CDIF28670_01452) - 1473927..1474634 (+) 708 WP_021393263.1 lantibiotic protection ABC transporter ATP-binding protein -
  CDIF28670_RS07320 (CDIF28670_01453) - 1474636..1475352 (+) 717 WP_022616933.1 lantibiotic immunity ABC transporter MutE/EpiE family permease subunit -
  CDIF28670_RS07325 (CDIF28670_01454) - 1475354..1476112 (+) 759 WP_021427419.1 lantibiotic immunity ABC transporter MutG family permease subunit -
  CDIF28670_RS07330 (CDIF28670_01455) - 1476247..1477635 (+) 1389 WP_021387809.1 two-component system sensor histidine kinase -
  CDIF28670_RS07335 (CDIF28670_01456) - 1477711..1478532 (-) 822 WP_021381893.1 pyridoxamine kinase -
  CDIF28670_RS07340 (CDIF28670_01457) - 1478783..1479937 (+) 1155 WP_009902426.1 amidase domain-containing protein -
  CDIF28670_RS07345 (CDIF28670_01458) - 1480120..1480320 (+) 201 WP_003419624.1 cold-shock protein -
  CDIF28670_RS07350 (CDIF28670_01459) - 1480741..1481169 (+) 429 WP_009889129.1 CBS domain-containing protein -
  CDIF28670_RS07355 (CDIF28670_01460) - 1481312..1482067 (-) 756 WP_009889131.1 peptidylprolyl isomerase -
  CDIF28670_RS07365 (CDIF28670_01462) - 1482768..1483274 (-) 507 WP_003428186.1 ImmA/IrrE family metallo-endopeptidase -
  CDIF28670_RS07370 (CDIF28670_01463) - 1483356..1483676 (-) 321 WP_003428185.1 XRE family transcriptional regulator -
  CDIF28670_RS07375 (CDIF28670_01464) - 1483845..1484042 (+) 198 WP_022616935.1 helix-turn-helix transcriptional regulator -
  CDIF28670_RS07380 (CDIF28670_01465) - 1484147..1484587 (+) 441 WP_022616936.1 hypothetical protein -
  CDIF28670_RS07385 (CDIF28670_01466) - 1484836..1485279 (+) 444 WP_003428180.1 phage tail terminator family protein -

Sequence


Protein


Download         Length: 348 a.a.        Molecular weight: 37377.68 Da        Isoelectric Point: 4.8236

>NTDB_id=153333 CDIF28670_RS07210 WP_003428218.1 1448125..1449171(+) (recA) [Clostridioides difficile strain DSM 28670]
MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTGAIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSC
VASAQKDGGIAAFIDAEHALDPVYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKFYSSVRLDVRKIDTIKQGDKVIG
SRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLLDIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEI
DEKVRAFYNLNEEHEESGTSVSKEIVEE

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=153333 CDIF28670_RS07210 WP_003428218.1 1448125..1449171(+) (recA) [Clostridioides difficile strain DSM 28670]
ATGAGTGTAGATCAAGAAAAATTAAAAGCGTTGAATGAAGCTTTAGGTAAAATTGAAAAAGATTTTGGTAAAGGTTCAGT
AATGAAATTGGGAGAAGCAACATCTATGTCTATAGATGTTATATCAACAGGAGCGATTGGTTTAGACATAGCTATTGGTA
TAGGAGGTCTACCTAGAGGGAGAATAGTTGAAGTATATGGTCCAGAATCTTCTGGTAAGACTACTGTTGCGCTTAGTTGT
GTAGCATCAGCTCAAAAAGATGGAGGAATAGCTGCATTTATAGATGCAGAACATGCACTTGACCCAGTATATGCAAAAGC
TTTGGGTGTGGATGTTGACAACCTAATAATATCTCAACCAGATACAGGTGAACAGGCTTTAGAGATAGCAGAGGCATTGA
TAAGAAGTGGAGCGATAGATATAATAGTAATAGACTCAGTAGCAGCATTAGTTCCAAAGGCTGAAATAGATGGAGATATG
GGTGATTCTCATGTAGGTTTACAAGCTAGACTTATGTCACAAGCACTTAGAAAGTTAACTGGTTCAATTAAAAAATCAAA
TTGTGTTGCTATATTTATAAACCAGTTAAGAGAGAAAGTAGGAATAATGTTTGGAAACCCAGAAACTACTACTGGAGGAC
GTGCACTAAAATTCTATTCATCAGTTAGATTGGATGTTAGAAAAATAGACACAATAAAACAAGGTGATAAAGTTATAGGT
AGTAGAACTAGAGTTAAAGTTGTTAAAAACAAAGTAGCACCACCATTTAAGCAAGCTGAATTTGATATAATGTATGGAGA
AGGGATTTCAAAAATTGGAGACCTTTTAGATATAGCTGCTGATGTAGATATAGTTAAAAAATCAGGTTCATGGTATAGTT
ACAATGATACTAAACTTGGACAAGGAAGAGAAAATGTTAAAAAATTCTTGGAGGATAATTTAGATTTAACTACTGAAATA
GATGAGAAAGTTAGAGCATTTTACAATTTAAATGAAGAACATGAAGAATCAGGTACTTCAGTATCAAAAGAAATTGTAGA
AGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A031WFI0

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Streptococcus mutans UA159

66.762

100

0.67

  recA Streptococcus mitis SK321

67.456

97.126

0.655

  recA Streptococcus pneumoniae Rx1

65.507

99.138

0.649

  recA Streptococcus pneumoniae R6

65.507

99.138

0.649

  recA Streptococcus pneumoniae D39

65.507

99.138

0.649

  recA Streptococcus pneumoniae TIGR4

65.507

99.138

0.649

  recA Streptococcus mitis NCTC 12261

68.085

94.54

0.644

  recA Bacillus subtilis subsp. subtilis str. 168

69.565

92.529

0.644

  recA Lactococcus lactis subsp. cremoris KW2

65.569

95.977

0.629

  recA Streptococcus pyogenes NZ131

66.972

93.966

0.629

  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

65.758

94.828

0.624

  recA Latilactobacillus sakei subsp. sakei 23K

64.955

95.115

0.618

  recA Neisseria gonorrhoeae MS11

66.049

93.103

0.615

  recA Neisseria gonorrhoeae MS11

66.049

93.103

0.615

  recA Neisseria gonorrhoeae strain FA1090

66.049

93.103

0.615

  recA Acinetobacter baylyi ADP1

64.396

92.816

0.598

  recA Pseudomonas stutzeri DSM 10701

63.889

93.103

0.595

  recA Helicobacter pylori 26695

61.976

95.977

0.595

  recA Helicobacter pylori strain NCTC11637

61.976

95.977

0.595

  recA Acinetobacter baumannii D1279779

63.777

92.816

0.592

  recA Vibrio cholerae O1 biovar El Tor strain E7946

62.048

95.402

0.592

  recA Vibrio cholerae strain A1552

62.048

95.402

0.592

  recA Glaesserella parasuis strain SC1401

64.174

92.241

0.592

  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

63.272

93.103

0.589

  recA Ralstonia pseudosolanacearum GMI1000

66.558

88.506

0.589

  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

60.681

92.816

0.563


Multiple sequence alignment