Detailed information    

insolico Bioinformatically predicted

Overview


Name   recA   Type   Machinery gene
Locus tag   ACJ74_RS10505 Genome accession   NZ_CP011915
Coordinates   2093603..2094664 (+) Length   353 a.a.
NCBI ID   WP_000963143.1    Uniprot ID   A0AAV3H3S5
Organism   Escherichia coli strain PSUO2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2086592..2128306 2093603..2094664 within 0


Gene organization within MGE regions


Location: 2086592..2128306
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ74_RS10460 (ACJ74_10120) gutQ 2086592..2087557 (-) 966 WP_001287423.1 arabinose-5-phosphate isomerase GutQ -
  ACJ74_RS10465 (ACJ74_10125) srlR 2087550..2088323 (-) 774 WP_000804544.1 glucitol operon DNA-binding transcriptional repressor SrlR -
  ACJ74_RS10470 (ACJ74_10130) gutM 2088390..2088749 (-) 360 WP_000252912.1 transcriptional regulator GutM -
  ACJ74_RS10475 (ACJ74_10135) srlD 2088855..2089634 (-) 780 WP_001077354.1 sorbitol-6-phosphate dehydrogenase -
  ACJ74_RS10480 (ACJ74_10140) srlB 2089638..2090009 (-) 372 WP_000216207.1 PTS glucitol/sorbitol transporter subunit IIA -
  ACJ74_RS10485 (ACJ74_10145) srlE 2090020..2090979 (-) 960 WP_000196936.1 PTS glucitol/sorbitol transporter subunit IIB -
  ACJ74_RS10490 (ACJ74_10150) srlA 2090976..2091539 (-) 564 WP_000573321.1 PTS glucitol/sorbitol transporter subunit IIC -
  ACJ74_RS10495 (ACJ74_10155) mltB 2091796..2092881 (+) 1086 WP_000476837.1 lytic murein transglycosylase B -
  ACJ74_RS10500 (ACJ74_10160) pncC 2093026..2093523 (+) 498 WP_000132231.1 nicotinamide-nucleotide amidase -
  ACJ74_RS10505 (ACJ74_10165) recA 2093603..2094664 (+) 1062 WP_000963143.1 recombinase RecA Machinery gene
  ACJ74_RS10510 (ACJ74_10170) recX 2094733..2095233 (+) 501 WP_000140506.1 recombination regulator RecX -
  ACJ74_RS10515 (ACJ74_10180) alaS 2095362..2097992 (+) 2631 WP_000047203.1 alanine--tRNA ligase -
  ACJ74_RS10520 (ACJ74_10185) csrA 2098227..2098412 (+) 186 WP_000906486.1 carbon storage regulator CsrA -
  ACJ74_RS10550 (ACJ74_10215) yqaB 2099601..2100167 (+) 567 WP_000273309.1 fructose-1-phosphate/6-phosphogluconate phosphatase -
  ACJ74_RS10555 (ACJ74_10220) yqaA 2100164..2100592 (+) 429 WP_001287462.1 YqaA family protein -
  ACJ74_RS10560 (ACJ74_10225) gshA 2100665..2102221 (+) 1557 WP_000611804.1 glutamate--cysteine ligase -
  ACJ74_RS10565 (ACJ74_10230) luxS 2102371..2102886 (+) 516 WP_001130208.1 S-ribosylhomocysteine lyase -
  ACJ74_RS10570 (ACJ74_10235) - 2102937..2104049 (-) 1113 WP_000638134.1 ATP-binding protein -
  ACJ74_RS10575 (ACJ74_10240) - 2104046..2104753 (-) 708 WP_001097131.1 RNA ligase family protein -
  ACJ74_RS10580 (ACJ74_10245) emrB 2105011..2106549 (-) 1539 WP_001296316.1 multidrug efflux MFS transporter permease subunit EmrB -
  ACJ74_RS10585 (ACJ74_10250) emrA 2106566..2107738 (-) 1173 WP_001295175.1 multidrug efflux MFS transporter periplasmic adaptor subunit EmrA -
  ACJ74_RS10590 (ACJ74_10255) emrR 2107865..2108395 (-) 531 WP_000378442.1 multidrug efflux transporter EmrAB transcriptional repressor EmrR -
  ACJ74_RS10595 (ACJ74_10260) ygaH 2108486..2108821 (-) 336 WP_000119749.1 L-valine transporter subunit YgaH -
  ACJ74_RS10600 (ACJ74_10265) ygaZ 2108811..2109548 (-) 738 WP_000445658.1 L-valine exporter subunit YgaZ -
  ACJ74_RS10605 (ACJ74_10270) - 2109672..2110856 (-) 1185 WP_000165688.1 MFS transporter -
  ACJ74_RS10610 (ACJ74_10275) proX 2111048..2112040 (-) 993 WP_001216530.1 glycine betaine/L-proline ABC transporter substrate-binding protein ProX -
  ACJ74_RS10615 (ACJ74_10280) proW 2112097..2113161 (-) 1065 WP_000774978.1 glycine betaine/L-proline ABC transporter permease ProW -
  ACJ74_RS10620 (ACJ74_10285) proV 2113154..2114356 (-) 1203 WP_000985490.1 glycine betaine/L-proline ABC transporter ATP-binding protein ProV -
  ACJ74_RS10630 (ACJ74_10290) nrdF 2114713..2115672 (-) 960 WP_000777936.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
  ACJ74_RS10635 (ACJ74_10295) nrdE 2115682..2117826 (-) 2145 WP_000246583.1 class 1b ribonucleoside-diphosphate reductase subunit alpha -
  ACJ74_RS10640 (ACJ74_10300) nrdI 2117799..2118209 (-) 411 WP_000080951.1 class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI -
  ACJ74_RS10645 (ACJ74_10305) nrdH 2118206..2118451 (-) 246 WP_001223230.1 glutaredoxin-like protein NrdH -
  ACJ74_RS10650 (ACJ74_10310) - 2118659..2119090 (-) 432 WP_000209793.1 carboxymuconolactone decarboxylase family protein -
  ACJ74_RS10655 (ACJ74_10315) - 2119178..2120512 (+) 1335 WP_001138429.1 PLP-dependent aminotransferase family protein -
  ACJ74_RS10660 (ACJ74_10320) ygaM 2120569..2120898 (-) 330 WP_001295174.1 DUF883 domain-containing protein -
  ACJ74_RS10665 (ACJ74_10325) ygaC 2121050..2121394 (+) 345 WP_000281320.1 DUF2002 family protein -
  ACJ74_RS10670 (ACJ74_10330) alaE 2121431..2121880 (-) 450 WP_000492656.1 L-alanine exporter AlaE -
  ACJ74_RS10675 (ACJ74_10335) stpA 2122547..2122951 (+) 405 WP_000115381.1 DNA-binding protein StpA -
  ACJ74_RS10680 (ACJ74_10340) ygaP 2122998..2123522 (-) 525 WP_001229462.1 thiosulfate sulfurtransferase YgaP -
  ACJ74_RS10685 (ACJ74_10345) ygaV 2123532..2123831 (-) 300 WP_000137284.1 ArsR/SmtB family transcription factor -
  ACJ74_RS10690 (ACJ74_10350) yqaE 2124014..2124172 (+) 159 WP_000508177.1 YqaE/Pmp3 family membrane protein -
  ACJ74_RS10695 (ACJ74_10355) kbp 2124256..2124705 (+) 450 WP_000522415.1 potassium binding protein Kbp -
  ACJ74_RS10700 (ACJ74_10360) csiR 2124706..2125368 (-) 663 WP_000156817.1 DNA-binding transcriptional regulator CsiR -
  ACJ74_RS10705 (ACJ74_10365) gabP 2125389..2126789 (-) 1401 WP_001298180.1 GABA permease -
  ACJ74_RS10710 (ACJ74_10370) gabT 2127026..2128306 (-) 1281 WP_000097653.1 4-aminobutyrate--2-oxoglutarate transaminase -

Sequence


Protein


Download         Length: 353 a.a.        Molecular weight: 37973.37 Da        Isoelectric Point: 4.8171

>NTDB_id=149286 ACJ74_RS10505 WP_000963143.1 2093603..2094664(+) (recA) [Escherichia coli strain PSUO2]
MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTGSLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQV
IAAAQREGKTCAFIDAEHALDPIYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRIGAVKEGENVVG
SETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELVDLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEI
EKKVRELLLSNPNSTPDFSVDDSEGVAETNEDF

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=149286 ACJ74_RS10505 WP_000963143.1 2093603..2094664(+) (recA) [Escherichia coli strain PSUO2]
ATGGCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGATTGAGAAACAATTTGGTAAAGGCTCCAT
CATGCGCCTGGGTGAAGACCGTTCCATGGATGTGGAAACCATCTCTACCGGTTCGCTTTCACTGGATATCGCGCTTGGGG
CAGGTGGTCTGCCGATGGGCCGTATCGTCGAAATCTACGGACCAGAATCTTCCGGTAAAACCACGCTGACGTTGCAGGTG
ATCGCCGCAGCGCAGCGCGAAGGTAAAACCTGTGCGTTTATCGATGCTGAACACGCGCTGGACCCAATCTACGCACGTAA
ACTGGGCGTCGATATCGACAACCTGCTGTGCTCCCAGCCGGATACCGGCGAGCAGGCACTGGAAATCTGTGACGCCCTGG
CGCGTTCTGGCGCAGTAGACGTTATCGTCGTTGACTCCGTGGCGGCACTGACGCCGAAAGCGGAAATCGAAGGCGAAATC
GGCGACTCTCACATGGGCCTTGCGGCACGTATGATGAGCCAGGCGATGCGTAAGCTGGCGGGTAACCTGAAGCAGTCCAA
CACGCTGCTGATCTTCATCAACCAGATCCGTATGAAAATTGGTGTGATGTTCGGTAACCCGGAAACCACTACCGGTGGTA
ACGCGCTGAAATTCTACGCCTCTGTTCGTCTCGACATCCGTCGTATCGGCGCGGTGAAAGAGGGCGAAAACGTGGTGGGT
AGCGAAACCCGCGTGAAAGTGGTGAAGAACAAAATCGCTGCGCCGTTTAAACAGGCTGAATTCCAGATCCTCTACGGCGA
AGGTATCAACTTCTACGGCGAGCTGGTTGACCTGGGCGTGAAAGAGAAGCTTATCGAGAAAGCAGGCGCGTGGTACAGCT
ACAAAGGTGAGAAGATCGGTCAGGGTAAAGCGAATGCGACTGCCTGGCTGAAAGATAACCCGGAAACCGCGAAAGAGATC
GAGAAGAAAGTACGTGAGTTGCTGCTGAGCAACCCGAACTCAACGCCGGATTTCTCTGTAGATGACAGCGAAGGCGTAGC
AGAAACTAACGAAGATTTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Vibrio cholerae O1 biovar El Tor strain E7946

84.29

93.768

0.79

  recA Vibrio cholerae strain A1552

84.29

93.768

0.79

  recA Pseudomonas stutzeri DSM 10701

74.184

95.467

0.708

  recA Acinetobacter baumannii D1279779

74.085

92.918

0.688

  recA Acinetobacter baylyi ADP1

73.78

92.918

0.686

  recA Glaesserella parasuis strain SC1401

69.452

98.3

0.683

  recA Neisseria gonorrhoeae MS11

69.018

92.351

0.637

  recA Neisseria gonorrhoeae MS11

69.018

92.351

0.637

  recA Neisseria gonorrhoeae strain FA1090

69.018

92.351

0.637

  recA Ralstonia pseudosolanacearum GMI1000

70.159

89.235

0.626

  recA Streptococcus mitis SK321

60.286

99.15

0.598

  recA Streptococcus mitis NCTC 12261

60.286

99.15

0.598

  recA Helicobacter pylori strain NCTC11637

61.721

95.467

0.589

  recA Helicobacter pylori 26695

61.424

95.467

0.586

  recA Streptococcus pneumoniae TIGR4

63.467

91.501

0.581

  recA Streptococcus pneumoniae Rx1

63.467

91.501

0.581

  recA Streptococcus pneumoniae D39

63.467

91.501

0.581

  recA Streptococcus pneumoniae R6

63.467

91.501

0.581

  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

62.654

91.785

0.575

  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

60.661

94.334

0.572

  recA Streptococcus mutans UA159

62.154

92.068

0.572

  recA Streptococcus pyogenes NZ131

62.154

92.068

0.572

  recA Bacillus subtilis subsp. subtilis str. 168

62.305

90.935

0.567

  recA Latilactobacillus sakei subsp. sakei 23K

61.538

92.068

0.567

  recA Lactococcus lactis subsp. cremoris KW2

61.61

91.501

0.564

  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

61.059

90.935

0.555


Multiple sequence alignment