Detailed information    

insolico Bioinformatically predicted

Overview


Name   recA   Type   Machinery gene
Locus tag   R2K01_RS13830 Genome accession   NZ_OX637968
Coordinates   2961871..2962917 (-) Length   348 a.a.
NCBI ID   WP_003428218.1    Uniprot ID   A0A031WFI0
Organism   Clostridioides difficile isolate L-NTCD03     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2914503..2971132 2961871..2962917 within 0


Gene organization within MGE regions


Location: 2914503..2971132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2K01_RS13595 - 2915366..2916352 (-) 987 WP_009889153.1 phage tail protein -
  R2K01_RS13600 - 2916353..2917051 (-) 699 WP_003428156.1 putative phage tail protein -
  R2K01_RS13605 - 2917048..2918100 (-) 1053 WP_038810938.1 baseplate J/gp47 family protein -
  R2K01_RS13610 - 2918093..2918521 (-) 429 WP_003428161.1 DUF2634 domain-containing protein -
  R2K01_RS13615 - 2918521..2918847 (-) 327 WP_070117399.1 DUF2577 family protein -
  R2K01_RS13620 - 2918863..2920395 (-) 1533 WP_070117398.1 NlpC/P60 family protein -
  R2K01_RS13625 - 2920677..2921093 (-) 417 WP_021375053.1 phage baseplate protein -
  R2K01_RS13630 - 2921099..2923498 (-) 2400 WP_003428170.1 hypothetical protein -
  R2K01_RS13635 - 2923498..2923665 (-) 168 WP_003428173.1 hypothetical protein -
  R2K01_RS13640 - 2923692..2924138 (-) 447 WP_070117396.1 phage tail assembly chaperone -
  R2K01_RS13645 - 2924252..2924680 (-) 429 WP_070117395.1 phage tail tube protein -
  R2K01_RS13650 - 2924694..2925758 (-) 1065 WP_070117394.1 phage tail sheath subtilisin-like domain-containing protein -
  R2K01_RS13655 - 2925763..2926206 (-) 444 WP_070117393.1 phage tail terminator family protein -
  R2K01_RS13660 - 2926455..2926895 (-) 441 WP_003428183.1 hypothetical protein -
  R2K01_RS13665 - 2926999..2927196 (-) 198 WP_003419641.1 helix-turn-helix transcriptional regulator -
  R2K01_RS13670 - 2927365..2927685 (+) 321 WP_003428185.1 XRE family transcriptional regulator -
  R2K01_RS13675 - 2927767..2928273 (+) 507 WP_003428186.1 ImmA/IrrE family metallo-endopeptidase -
  R2K01_RS13680 - 2928437..2928853 (+) 417 WP_003428188.1 hypothetical protein -
  R2K01_RS13685 - 2928974..2929729 (+) 756 WP_021387811.1 peptidylprolyl isomerase -
  R2K01_RS13690 - 2929872..2930300 (-) 429 WP_009889129.1 CBS domain-containing protein -
  R2K01_RS13695 - 2930721..2930921 (-) 201 WP_003419624.1 cold-shock protein -
  R2K01_RS13700 - 2931104..2932258 (-) 1155 WP_009902426.1 amidase domain-containing protein -
  R2K01_RS13705 - 2932509..2933330 (+) 822 WP_003428191.1 pyridoxamine kinase -
  R2K01_RS13710 - 2933406..2934794 (-) 1389 WP_022616934.1 two-component system sensor histidine kinase -
  R2K01_RS13715 - 2934929..2935687 (-) 759 WP_021427419.1 lantibiotic immunity ABC transporter MutG family permease subunit -
  R2K01_RS13720 - 2935689..2936405 (-) 717 WP_022616933.1 lantibiotic immunity ABC transporter MutE/EpiE family permease subunit -
  R2K01_RS13725 - 2936407..2937114 (-) 708 WP_021393263.1 lantibiotic protection ABC transporter ATP-binding protein -
  R2K01_RS13730 - 2937230..2937766 (-) 537 WP_003428196.1 lipoprotein -
  R2K01_RS13735 - 2937868..2938938 (-) 1071 WP_070117391.1 DUF4097 family beta strand repeat-containing protein -
  R2K01_RS13740 - 2938931..2939989 (-) 1059 WP_003428198.1 DUF1700 domain-containing protein -
  R2K01_RS13745 - 2939976..2940308 (-) 333 WP_009889116.1 PadR family transcriptional regulator -
  R2K01_RS13750 - 2940507..2941628 (+) 1122 WP_003428201.1 MGDG synthase family glycosyltransferase -
  R2K01_RS13755 - 2941756..2943654 (-) 1899 WP_003428202.1 glutamine synthetase -
  R2K01_RS13760 - 2943969..2944745 (+) 777 WP_009896237.1 3'-5' exonuclease -
  R2K01_RS13765 - 2944837..2945589 (-) 753 WP_003428204.1 exodeoxyribonuclease III -
  R2K01_RS13770 galT 2945742..2946692 (-) 951 WP_003428205.1 galactose-1-phosphate uridylyltransferase -
  R2K01_RS13775 - 2947077..2948273 (+) 1197 WP_003438357.1 pyridoxal phosphate-dependent aminotransferase -
  R2K01_RS13780 - 2948364..2949731 (-) 1368 WP_330388089.1 6-phospho-alpha-glucosidase -
  R2K01_RS13785 - 2949864..2950616 (-) 753 WP_009892977.1 MurR/RpiR family transcriptional regulator -
  R2K01_RS13790 - 2950764..2952341 (-) 1578 WP_074048084.1 PTS transporter subunit EIIC -
  R2K01_RS13795 - 2952745..2953197 (-) 453 WP_003428210.1 MarR family winged helix-turn-helix transcriptional regulator -
  R2K01_RS13800 purB 2953508..2954953 (-) 1446 WP_070117388.1 adenylosuccinate lyase -
  R2K01_RS13805 - 2955097..2956005 (-) 909 WP_009889110.1 tyrosine-type recombinase/integrase -
  R2K01_RS13810 - 2956262..2957086 (+) 825 WP_009905410.1 undecaprenyl-diphosphate phosphatase -
  R2K01_RS13815 lepB 2957126..2957656 (+) 531 WP_021387803.1 signal peptidase I -
  R2K01_RS13820 - 2957738..2959519 (-) 1782 WP_003428215.1 UvrD-helicase domain-containing protein -
  R2K01_RS13825 rny 2960154..2961695 (-) 1542 WP_003428216.1 ribonuclease Y -
  R2K01_RS13830 recA 2961871..2962917 (-) 1047 WP_003428218.1 recombinase RecA Machinery gene
  R2K01_RS13835 pgsA 2963205..2963747 (-) 543 WP_021387802.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  R2K01_RS13840 rimO 2963734..2965068 (-) 1335 WP_003428221.1 30S ribosomal protein S12 methylthiotransferase RimO -
  R2K01_RS13845 mnmH 2965062..2966120 (-) 1059 WP_003428222.1 tRNA 2-selenouridine(34) synthase MnmH -
  R2K01_RS13850 - 2966123..2968531 (-) 2409 WP_070117387.1 FtsK/SpoIIIE family DNA translocase -
  R2K01_RS13855 - 2968654..2969388 (-) 735 WP_003428224.1 ClpP family protease -
  R2K01_RS13860 dapG 2969501..2970724 (-) 1224 WP_003428226.1 aspartate kinase -
  R2K01_RS13865 - 2970734..2970979 (-) 246 WP_003419503.1 YlmC/YmxH family sporulation protein -

Sequence


Protein


Download         Length: 348 a.a.        Molecular weight: 37377.68 Da        Isoelectric Point: 4.8236

>NTDB_id=1160101 R2K01_RS13830 WP_003428218.1 2961871..2962917(-) (recA) [Clostridioides difficile isolate L-NTCD03]
MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTGAIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSC
VASAQKDGGIAAFIDAEHALDPVYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKFYSSVRLDVRKIDTIKQGDKVIG
SRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLLDIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEI
DEKVRAFYNLNEEHEESGTSVSKEIVEE

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=1160101 R2K01_RS13830 WP_003428218.1 2961871..2962917(-) (recA) [Clostridioides difficile isolate L-NTCD03]
ATGAGTGTAGATCAAGAAAAATTAAAAGCGTTGAATGAAGCTTTAGGTAAAATTGAAAAAGATTTTGGTAAAGGTTCAGT
AATGAAATTGGGAGAAGCAACATCTATGTCTATAGATGTTATATCAACAGGAGCGATTGGTTTAGACATAGCTATTGGTA
TAGGAGGTCTACCTAGAGGGAGAATAGTTGAAGTATATGGTCCAGAATCTTCTGGTAAGACTACTGTTGCGCTTAGTTGT
GTAGCATCAGCTCAAAAAGATGGAGGAATAGCTGCATTTATAGATGCAGAACATGCACTTGACCCAGTATATGCAAAAGC
TTTGGGTGTGGATGTTGACAACCTAATAATATCTCAACCAGATACAGGTGAACAGGCTTTAGAGATAGCAGAGGCATTGA
TAAGAAGTGGAGCGATAGATATAATAGTAATAGACTCAGTAGCAGCATTAGTTCCAAAGGCTGAAATAGATGGAGATATG
GGTGATTCTCATGTAGGTTTACAAGCTAGACTTATGTCACAAGCACTTAGAAAGTTAACTGGTTCAATTAAAAAATCAAA
TTGTGTTGCTATATTTATAAACCAGTTAAGAGAGAAAGTAGGAATAATGTTTGGAAACCCAGAAACTACTACTGGAGGAC
GTGCATTAAAATTCTATTCATCAGTTAGATTGGATGTTAGAAAAATAGACACAATAAAACAAGGTGATAAAGTTATAGGT
AGTAGAACTAGAGTTAAAGTTGTTAAAAACAAAGTAGCACCACCATTTAAGCAAGCTGAATTTGATATAATGTATGGAGA
AGGGATTTCAAAAATTGGAGACCTTTTAGATATAGCTGCTGATGTAGATATAGTTAAAAAATCAGGTTCATGGTATAGTT
ACAATGATACTAAACTTGGACAAGGAAGAGAAAATGTTAAAAAATTCTTGGAGGATAATTTAGATTTAACTACTGAAATA
GATGAGAAAGTTAGAGCATTTTACAATTTAAATGAAGAACATGAAGAATCAGGTACTTCAGTATCAAAAGAAATTGTAGA
AGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A031WFI0

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Streptococcus mutans UA159

66.762

100

0.67

  recA Streptococcus mitis SK321

67.456

97.126

0.655

  recA Streptococcus pneumoniae Rx1

65.507

99.138

0.649

  recA Streptococcus pneumoniae R6

65.507

99.138

0.649

  recA Streptococcus pneumoniae D39

65.507

99.138

0.649

  recA Streptococcus pneumoniae TIGR4

65.507

99.138

0.649

  recA Streptococcus mitis NCTC 12261

68.085

94.54

0.644

  recA Bacillus subtilis subsp. subtilis str. 168

69.565

92.529

0.644

  recA Lactococcus lactis subsp. cremoris KW2

65.569

95.977

0.629

  recA Streptococcus pyogenes NZ131

66.972

93.966

0.629

  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

65.758

94.828

0.624

  recA Latilactobacillus sakei subsp. sakei 23K

64.955

95.115

0.618

  recA Neisseria gonorrhoeae MS11

66.049

93.103

0.615

  recA Neisseria gonorrhoeae MS11

66.049

93.103

0.615

  recA Neisseria gonorrhoeae strain FA1090

66.049

93.103

0.615

  recA Acinetobacter baylyi ADP1

64.396

92.816

0.598

  recA Pseudomonas stutzeri DSM 10701

63.889

93.103

0.595

  recA Helicobacter pylori 26695

61.976

95.977

0.595

  recA Helicobacter pylori strain NCTC11637

61.976

95.977

0.595

  recA Acinetobacter baumannii D1279779

63.777

92.816

0.592

  recA Vibrio cholerae O1 biovar El Tor strain E7946

62.048

95.402

0.592

  recA Vibrio cholerae strain A1552

62.048

95.402

0.592

  recA Glaesserella parasuis strain SC1401

64.174

92.241

0.592

  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

63.272

93.103

0.589

  recA Ralstonia pseudosolanacearum GMI1000

66.558

88.506

0.589

  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

60.681

92.816

0.563


Multiple sequence alignment