Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | pezAT/- |
| Location | 934826..936120 | Replicon | chromosome |
| Accession | NC_003098 | ||
| Organism | Streptococcus pneumoniae R6 | ||
Toxin (Protein)
| Gene name | pezT | Uniprot ID | R4H7C0 |
| Locus tag | SPR_RS04765 | Protein ID | WP_000405357.1 |
| Coordinates | 935359..936120 (+) | Length | 254 a.a. |
Antitoxin (Protein)
| Gene name | pezA | Uniprot ID | B6ZCC4 |
| Locus tag | SPR_RS04760 | Protein ID | WP_000579612.1 |
| Coordinates | 934883..935359 (+) | Length | 159 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SPR_RS04735 (spr0946) | 929956..930810 | + | 855 | WP_000900020.1 | ADP-ribosylglycohydrolase family protein | - |
| SPR_RS04740 (spr0947) | 930922..931806 | + | 885 | WP_000710119.1 | diacylglycerol kinase family lipid kinase | - |
| SPR_RS04745 (spr0948) | 931969..933708 | + | 1740 | WP_000423451.1 | glycoside hydrolase family 13 protein | - |
| SPR_RS04750 (spr0949) | 933794..934111 | + | 318 | WP_000995820.1 | hypothetical protein | - |
| SPR_RS04755 (spr0950) | 934157..934438 | + | 282 | WP_000266233.1 | hypothetical protein | - |
| SPR_RS04760 (spr0951) | 934883..935359 | + | 477 | WP_000579612.1 | helix-turn-helix domain-containing protein | Antitoxin |
| SPR_RS04765 (spr0952) | 935359..936120 | + | 762 | WP_000405357.1 | zeta toxin family protein | Toxin |
| SPR_RS04775 (spr0955) | 938136..938495 | + | 360 | WP_000529543.1 | hypothetical protein | - |
| SPR_RS04780 (spr0956) | 938505..938834 | + | 330 | WP_000263037.1 | MobC family plasmid mobilization relaxosome protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Genomic island | - | - | 920239..953000 | 32761 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 254 a.a. Molecular weight: 29044.10 Da Isoelectric Point: 7.1307
>T6282 WP_000405357.1 NC_003098:935359-936120 [Streptococcus pneumoniae R6]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK
Download Length: 762 bp
>T6282 NC_003098:935359-936120 [Streptococcus pneumoniae R6]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAACAACCTATAGCGATTTTGCTTGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGCGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGATAGCGTTGAATACACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGGGATACAATCTTTTGATCGAGGGAACTTTACGAACAATTGATGTTCCAAAGAAAACGGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTGGCCTTAATTGCGACAAAGCCTGAATTGTCGTATCTAAGTACTCTTATCCGTTATGAAGAA
CTGTACATTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
CACACGAAAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTACTCTTTGGGGAGTGGAGTCAGGTAGAGAAGGAGATGTTG
CAGGTGGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAACAACCTATAGCGATTTTGCTTGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGCGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGATAGCGTTGAATACACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGGGATACAATCTTTTGATCGAGGGAACTTTACGAACAATTGATGTTCCAAAGAAAACGGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTGGCCTTAATTGCGACAAAGCCTGAATTGTCGTATCTAAGTACTCTTATCCGTTATGAAGAA
CTGTACATTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
CACACGAAAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTACTCTTTGGGGAGTGGAGTCAGGTAGAGAAGGAGATGTTG
CAGGTGGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA
Antitoxin
Download Length: 159 a.a. Molecular weight: 18245.68 Da Isoelectric Point: 4.4188
>AT6282 WP_000579612.1 NC_003098:934883-935359 [Streptococcus pneumoniae R6]
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA
Download Length: 477 bp
>AT6282 NC_003098:934883-935359 [Streptococcus pneumoniae R6]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCCGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACTAGTTCAGTCTCTACAGAATTAATTGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCCGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACTAGTTCAGTCTCTACAGAATTAATTGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10019 | Streptococcus pneumoniae TIGR4 |
99.209 |
100 |
0.992 |
| T6080 | Streptococcus pneumoniae gamPNI0373 |
97.233 |
100 |
0.972 |
| T6280 | Streptococcus suis 05ZYH33 |
68.924 |
99.209 |
0.684 |
| T6037 | Enterococcus faecium |
68.526 |
99.209 |
0.68 |
| T6024 | Streptococcus pyogenes |
42.593 |
85.375 |
0.364 |
| T6079 | Streptococcus pyogenes |
42.593 |
85.375 |
0.364 |
| T6104 | Enterococcus faecalis |
42.593 |
85.375 |
0.364 |
| T6092 | Staphylococcus aureus |
42.593 |
85.375 |
0.364 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6080 | Streptococcus pneumoniae gamPNI0373 |
100 |
100 |
1 |
| AT10019 | Streptococcus pneumoniae TIGR4 |
98.734 |
100 |
0.987 |
| AT6037 | Enterococcus faecium |
81.013 |
100 |
0.81 |
| AT6280 | Streptococcus suis 05ZYH33 |
81.013 |
100 |
0.81 |
Multiple sequence alignment
References
(1) Wai T Chan et al. (2016) The Streptococcus pneumoniae pezAT Toxin-Antitoxin System Reduces β-Lactam Resistance and Genetic Competence. Frontiers in Microbiology 7:1322. [PubMed:27610103]