6024

Detailed information of TA system    

experimentalExperimentally validated    

Overview


TA module


Type II Classification (family/domain) omega-epsilon-zeta/-
Location 6512..7881 Replicon plasmid pSM19035
Accession NC_006979
Organism Streptococcus pyogenes

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag HS217_RS00045 Protein ID WP_001284311.1
Coordinates 7018..7881 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag HS217_RS00040 Protein ID WP_000301765.1
Coordinates 6744..7016 (+) Length 91 a.a.

Regulator (Protein)


Gene name omega Uniprot ID Q0H3F5
Locus tag HS217_RS00035 Protein ID WP_001835296.1
Coordinates 6512..6727 (+) Length 72 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS217_RS00015 (pSM19035_003) 2476..2646 + 171 WP_000713594.1 hypothetical protein -
HS217_RS00020 (pSM19035_004) 2660..3277 + 618 WP_001062589.1 recombinase family protein -
HS217_RS00025 (pSM19035_005) 3277..5421 + 2145 WP_000108741.1 type IA DNA topoisomerase -
HS217_RS00030 (pSM19035_006) 5524..6420 + 897 WP_001835294.1 ParA family protein -
HS217_RS00035 (pSM19035_007) 6512..6727 + 216 WP_001835296.1 peptide-binding protein Regulator
HS217_RS00040 (pSM19035_008) 6744..7016 + 273 WP_000301765.1 antitoxin Antitoxin
HS217_RS00045 (pSM19035_009) 7018..7881 + 864 WP_001284311.1 zeta toxin family protein Toxin
HS217_RS00055 (pSM19035_010) 8367..8447 + 81 Protein_9 23S rRNA methyltransferase attenuation leader peptide -
HS217_RS00060 (pSM19035_011) 8570..9307 + 738 WP_063844598.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
HS217_RS00065 (pSM19035_012) 9570..10433 - 864 WP_001284311.1 zeta toxin family protein -
HS217_RS00070 (pSM19035_013) 10435..10707 - 273 WP_000301765.1 antitoxin -
HS217_RS00075 (pSM19035_014) 10724..10939 - 216 WP_001835296.1 peptide-binding protein -
HS217_RS00080 (pSM19035_015) 11031..11927 - 897 WP_001835294.1 ParA family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) - 1..28975 28975


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-214)

Antitoxin

(2-90)


Sequences


Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T6024 WP_001284311.1 NC_006979:7018-7881 [Streptococcus pyogenes]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T6024 NC_006979:7018-7881 [Streptococcus pyogenes]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT6024 WP_000301765.1 NC_006979:6744-7016 [Streptococcus pyogenes]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT6024 NC_006979:6744-7016 [Streptococcus pyogenes]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Regulator


Download         Length: 72 a.a.        Molecular weight: 7956.26 Da        Isoelectric Point: 10.1709

>RE6024 WP_001835296.1 NC_006979:6512-6727 [Streptococcus pyogenes]
VIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAKNGGNVKEVMDQALEEYIRKYLPDKL

Download         Length: 216 bp

>RE6024 NC_006979:6512-6727 [Streptococcus pyogenes]
GTGATTGTGGGAAATTTAGGCGCACAAAAAGCAAAACGAAATGATACACCAATCAGTGCAAAAAAAGATATAATGGGAGA
TAAGACGGTTCGTGTTCGTGCTGACTTGCACCATATCATAAAAATCGAAACAGCAAAGAATGGCGGAAACGTAAAAGAAG
TTATGGATCAAGCCTTAGAAGAATATATACGGAAATATTTACCTGACAAACTTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6092 Staphylococcus aureus

100

100

1

T6079 Streptococcus pyogenes

100

100

1

T6104 Enterococcus faecalis

97.561

100

0.976

T6280 Streptococcus suis 05ZYH33

43.75

86.822

0.38

T6037 Enterococcus faecium

40.167

93.359

0.375

T6080 Streptococcus pneumoniae gamPNI0373

43.318

85.771

0.372

T6282 Streptococcus pneumoniae R6

42.593

85.375

0.364

T10019 Streptococcus pneumoniae TIGR4

42.13

85.375

0.36


Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT6092 Staphylococcus aureus

100

100

1

AT6104 Enterococcus faecalis

100

100

1

AT6079 Streptococcus pyogenes

100

100

1


Multiple sequence alignment    




Structures


Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X


Antitoxin

Source ID Structure
AlphaFold DB A0A829F0A3

References


(1) Urszula Zielenkiewicz et al. (2005) The toxin-antitoxin system of the streptococcal plasmid pSM19035. Journal of Bacteriology 187(17):6094-105. [PubMed:16109951] experimental

(2) Ana G Camacho et al. (2002) In vitro and in vivo stability of the epsilon2zeta2 protein complex of the broad host-range Streptococcus pyogenes pSM19035 addiction system. Biological Chemistry 383(11):1701-13. [PubMed:12530535] experimental

(3) Virginia S Lioy et al. (2006) pSM19035-encoded zeta toxin induces stasis followed by death in a subpopulation of cells. Microbiology (Reading, England) 152(Pt 8):2365-2379. [PubMed:16849801] experimental

(4) Virginia S Lioy et al. (2010) Plasmid pSM19035, a model to study stable maintenance in Firmicutes. Plasmid 64(1):1-17. [PubMed:20403380]

(5) A Meinhart et al. (2001) Crystallization and preliminary X-ray diffraction studies of the epsilonzeta addiction system encoded by Streptococcus pyogenes plasmid pSM19035. Acta Crystallographica. Section D, Biological Crystallography 57(Pt 5):745-7. [PubMed:11320325] 3D_structure

(6) Anton Meinhart et al. (2003) Crystal structure of the plasmid maintenance system epsilon/zeta: functional mechanism of toxin zeta and inactivation by epsilon 2 zeta 2 complex formation. Proceedings of The National Academy of Sciences of The United States of America 100(4):1661-6. [PubMed:12571357] 3D_structure

(7) Virginia S Lioy et al. (2010) A toxin-antitoxin module as a target for antimicrobial development. Plasmid 63(1):31-9. [PubMed:19800365] experimental

(8) Urszula Zielenkiewicz et al. (2009) In vivo interactions between toxin-antitoxin proteins epsilon and zeta of streptococcal plasmid pSM19035 in Saccharomyces cerevisiae. Journal of Bacteriology 191(11):3677-84. [PubMed:19346303] experimental

(9) Iwona Brzozowska et al. (2014) The ClpXP protease is responsible for the degradation of the Epsilon antidote to the Zeta toxin of the streptococcal pSM19035 plasmid. The Journal of Biological Chemistry 289(11):7514-23. [PubMed:24492616] experimental

(10) Iwona Brzozowska et al. (2012) Functioning of the TA cassette of streptococcal plasmid pSM19035 in various Gram-positive bacteria. Plasmid 68(1):51-60. [PubMed:22309878]

(11) Andrea Volante et al. (2014) The Interplay between Different Stability Systems Contributes to Faithful Segregation: Streptococcus pyogenes pSM19035 as a Model. Microbiology Spectrum 2(4):PLAS-0007-2013. [PubMed:26104212] experimental


experimental experimental literature
3D_structure protein structure literature