Detailed information of TA system TA006080

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/Xre(antitoxin)
• Location: 1049109..1050400
• Replicon: chromosome
• Accession: NC_018630
• Organism: Streptococcus pneumoniae gamPNI0373

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: HMPREF1038_RS05580
• Protein ID: WP_000405367.1
• Coordinates: 1049109..1049870 (-)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: B6ZCC4
• Locus tag: HMPREF1038_RS05585
• Protein ID: WP_000579612.1
• Coordinates: 1049870..1050346 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HMPREF1038_RS11710	1044532..1046385	-	1854	Protein_1048	relaxase/mobilization nuclease domain-containing protein	-
HMPREF1038_RS05565 (HMPREF1038_01129)	1046372..1046670	-	299	Protein_1049	MobC family plasmid mobilization relaxosome protein	-
HMPREF1038_RS05570 (HMPREF1038_01130)	1046746..1047105	-	360	WP_000003746.1	hypothetical protein	-
HMPREF1038_RS05575 (HMPREF1038_01131)	1047924..1049081	-	1158	WP_001213953.1	hypothetical protein	-
HMPREF1038_RS05580 (HMPREF1038_01132)	1049109..1049870	-	762	WP_000405367.1	zeta toxin family protein	Toxin
HMPREF1038_RS05585 (HMPREF1038_01133)	1049870..1050346	-	477	WP_000579612.1	helix-turn-helix domain-containing protein	Antitoxin
HMPREF1038_RS05590 (HMPREF1038_01134)	1050791..1051246	-	456	WP_000500471.1	hypothetical protein	-
HMPREF1038_RS05600 (HMPREF1038_01135)	1051521..1053258	-	1738	Protein_1055	alpha-glycosidase	-
HMPREF1038_RS05605 (HMPREF1038_01137)	1053421..1054305	-	885	WP_001844663.1	diacylglycerol kinase family lipid kinase	-
HMPREF1038_RS05610 (HMPREF1038_01138)	1054417..1055271	-	855	WP_000900020.1	ADP-ribosylglycohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_012467 Streptococcus pneumoniae P1031 Genomic island TA023658 948920..980083 0.005 View NZ_CP031247 Streptococcus pneumoniae strain M23734 Genomic island TA108716 507165..538210 0.005 View NZ_LN831051 Streptococcus pneumoniae strain NCTC7465 Genomic island TA285643 1610627..1641675 0.004	inside	Genomic island	-	-	1035089..1069396	34307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29123.21 Da
• Isoelectric Point: 8.6519

>T6080 WP_000405367.1 NC_018630:c1049870-1049109 [Streptococcus pneumoniae gamPNI0373]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLHELLFGEWSQVEKEML
RVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T6080 NC_018630:c1049870-1049109 [Streptococcus pneumoniae gamPNI0373]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAACAACCTATAGCGATTTTGCTTGGAGGTCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGTGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGACAGTGTAGAATATACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGAGATACAATCTTTTGATAGAGGGAACTTTACGAACAGTTGATGTTCCAAAGAAAACGGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTAGCCTTGATTGCGACAAAGCCTAAGCTGTCCTATCTGAGCACTCTTATTCGATACGAAGAA
CTGTACGCTATCAATCCAAATCAAGCACGCGCAACACCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGACAATTGGAAGAACTAGCTATTTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCATGAGTTATTGTTTGGAGAGTGGAGTCAGGTAGAAAAGGAGATGTTG
AGGGTAGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18245.68 Da
• Isoelectric Point: 4.4188

>AT6080 WP_000579612.1 NC_018630:c1050346-1049870 [Streptococcus pneumoniae gamPNI0373]
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT6080 NC_018630:c1050346-1049870 [Streptococcus pneumoniae gamPNI0373]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCCGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACGAGTTCAGTCTCTACGGAATTAATTGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T10019	Streptococcus pneumoniae TIGR4	98.024	100	0.98
T6282	Streptococcus pneumoniae R6	97.233	100	0.972
T6280	Streptococcus suis 05ZYH33	68.127	99.209	0.676
T6037	Enterococcus faecium	68.127	99.209	0.676
T6092	Staphylococcus aureus	43.318	85.771	0.372
T6079	Streptococcus pyogenes	43.318	85.771	0.372
T6104	Enterococcus faecalis	43.318	85.771	0.372
T6024	Streptococcus pyogenes	43.318	85.771	0.372

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT6282	Streptococcus pneumoniae R6	100	100	1
AT10019	Streptococcus pneumoniae TIGR4	98.734	100	0.987
AT6037	Enterococcus faecium	81.013	100	0.81
AT6280	Streptococcus suis 05ZYH33	81.013	100	0.81

Multiple sequence alignment

Structures

Antitoxin

• AlphaFold DB: B6ZCC4

References

(1) Hannes Mutschler et al. (2010) Assembly dynamics and stability of the pneumococcal epsilon zeta antitoxin toxin (PezAT) system from Streptococcus pneumoniae. The Journal of Biological Chemistry 285(28):21797-806. [PubMed:20442221]


 experimental literature