Detailed information of TA system TA006104

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/-
• Location: 3461..4598
• Replicon: plasmid pW9-2
• Accession: NG_041566
• Organism: Enterococcus faecalis

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: pJHW9-2_006
• Protein ID: YP_009090346.1
• Coordinates: 3735..4598 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: -
• Locus tag: pJHW9-2_005
• Protein ID: YP_009090345.1
• Coordinates: 3461..3733 (+)
• Length: 91 a.a.

Regulator (Protein)

• Gene name: omega
• Uniprot ID: -
• Locus tag: pJHW9-2_004
• Protein ID: YP_009090344.1
• Coordinates: 3229..3444 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
pJHW9-2_001	76..813	+	738	YP_009090341.1	ErmB	-
pJHW9-2_002	1345..1767	-	423	YP_009090342.1	hyprothetical protein	-
pJHW9-2_003	2241..3137	+	897	YP_009090343.1	CobQ/CobB/MinD/ParA nucleotide binding domain protein	-
pJHW9-2_004	3229..3444	+	216	YP_009090344.1	omega transcriptional repressor	Regulator
pJHW9-2_005	3461..3733	+	273	YP_009090345.1	epsilon antitoxin	Antitoxin
pJHW9-2_006	3735..4598	+	864	YP_009090346.1	zeta toxin	Toxin
pJHW9-2_007	5038..5355	+	318	YP_009090347.1	ssDNA binding protein	-
pJHW9-2_008	5878..6384	+	507	YP_009090348.1	hypothetical protein	-
pJHW9-2_009	6759..7931	+	1173	YP_009090349.1	transposase	-
pJHW9-2_010	8364..9413	-	1050	YP_009090350.1	rRNA methylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / cfr	-	1..25761	25761

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32370.87 Da
• Isoelectric Point: 6.6610

>T6104 YP_009090346.1 NG_041566:3735-4598 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T6104 NG_041566:3735-4598 [Enterococcus faecalis]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAAAATCGCTTAAATGATAATTTAGAAGAATTGGTTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACGAGTTTGCGATCAGCAATTC
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGCG
AAACTTTATGAGAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTAAG
CGATCAAGGCTATAACTTGGTGATTGAAGGTACAGGACGAACAACAGACGTTCCCATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGACTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT6104 YP_009090345.1 NG_041566:3461-3733 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT6104 NG_041566:3461-3733 [Enterococcus faecalis]
ATGGCAGTTACATATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGCTATTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Regulator

Protein

• Length: 72 a.a.
• Molecular weight: 7956.31 Da
• Isoelectric Point: 10.3185

>RE6104 YP_009090344.1 NG_041566:3229-3444 [Enterococcus faecalis]
VIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAKNGGNVKEVMDKALEEYIRKYLPDKL

Nucleotide

• Length: 216 bp

>RE6104 NG_041566:3229-3444 [Enterococcus faecalis]
GTGATTGTGGGAAATTTAGGCGCACAAAAAGCAAAACGAAATGATACACCAATCAGTGCAAAAAAAGATATAATGGGAGA
TAAGACGGTTCGTGTTCGTGCTGACTTGCACCATATCATAAAAATCGAAACAGCAAAGAATGGCGGAAACGTAAAAGAAG
TTATGGATAAAGCCTTAGAAGAATATATTCGGAAATATTTACCTGACAAACTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6024	Streptococcus pyogenes	97.561	100	0.976
T6079	Streptococcus pyogenes	97.561	100	0.976
T6092	Staphylococcus aureus	97.561	100	0.976
T6037	Enterococcus faecium	40.167	93.359	0.375
T6080	Streptococcus pneumoniae gamPNI0373	43.318	85.771	0.372
T6282	Streptococcus pneumoniae R6	42.593	85.375	0.364
T10019	Streptococcus pneumoniae TIGR4	42.13	85.375	0.36
T6280	Streptococcus suis 05ZYH33	44.231	80.62	0.357

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT6079	Streptococcus pyogenes	100	100	1
AT6092	Staphylococcus aureus	100	100	1
AT6024	Streptococcus pyogenes	100	100	1

Multiple sequence alignment

References

(1) Yang Liu et al. (2013) Transferable multiresistance plasmids carrying cfr in Enterococcus spp. from swine and farm environment. Antimicrobial Agents And Chemotherapy 57(1):42-8. [PubMed:23070165]


 experimental literature