Detailed information of TA system
Experimentally validatedOverview
TA module
Type | II | Classification (family/domain) | omega-epsilon-zeta/- |
Location | 9570..10707 | Replicon | plasmid pSM19035 |
Accession | NC_006979 | ||
Organism | Streptococcus pyogenes |
Toxin (Protein)
Gene name | zeta | Uniprot ID | Q54944 |
Locus tag | HS217_RS00065 | Protein ID | WP_001284311.1 |
Coordinates | 9570..10433 (-) | Length | 288 a.a. |
Antitoxin (Protein)
Gene name | epsilon | Uniprot ID | R2XCR7 |
Locus tag | HS217_RS00070 | Protein ID | WP_000301765.1 |
Coordinates | 10435..10707 (-) | Length | 91 a.a. |
Regulator (Protein)
Gene name | omega | Uniprot ID | Q0H3F5 |
Locus tag | HS217_RS00075 | Protein ID | WP_001835296.1 |
Coordinates | 10724..10939 (-) | Length | 72 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
HS217_RS00030 (pSM19035_006) | 5524..6420 | + | 897 | WP_001835294.1 | ParA family protein | - |
HS217_RS00035 (pSM19035_007) | 6512..6727 | + | 216 | WP_001835296.1 | peptide-binding protein | - |
HS217_RS00040 (pSM19035_008) | 6744..7016 | + | 273 | WP_000301765.1 | antitoxin | - |
HS217_RS00045 (pSM19035_009) | 7018..7881 | + | 864 | WP_001284311.1 | zeta toxin family protein | - |
HS217_RS00055 (pSM19035_010) | 8367..8447 | + | 81 | Protein_9 | 23S rRNA methyltransferase attenuation leader peptide | - |
HS217_RS00060 (pSM19035_011) | 8570..9307 | + | 738 | WP_063844598.1 | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | - |
HS217_RS00065 (pSM19035_012) | 9570..10433 | - | 864 | WP_001284311.1 | zeta toxin family protein | Toxin |
HS217_RS00070 (pSM19035_013) | 10435..10707 | - | 273 | WP_000301765.1 | antitoxin | Antitoxin |
HS217_RS00075 (pSM19035_014) | 10724..10939 | - | 216 | WP_001835296.1 | peptide-binding protein | Regulator |
HS217_RS00080 (pSM19035_015) | 11031..11927 | - | 897 | WP_001835294.1 | ParA family protein | - |
HS217_RS00085 (pSM19035_016) | 12030..14174 | - | 2145 | WP_000108741.1 | type IA DNA topoisomerase | - |
HS217_RS00090 (pSM19035_017) | 14174..14791 | - | 618 | WP_001062589.1 | recombinase family protein | - |
HS217_RS00095 (pSM19035_018) | 14805..14975 | - | 171 | WP_000713594.1 | hypothetical protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | erm(B) | - | 1..28975 | 28975 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 288 a.a. Molecular weight: 32404.02 Da Isoelectric Point: 6.9964
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI
Download Length: 864 bp
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA
Antitoxin
Download Length: 91 a.a. Molecular weight: 10721.25 Da Isoelectric Point: 6.4676
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA
Download Length: 273 bp
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA
Regulator
Download Length: 72 a.a. Molecular weight: 7956.26 Da Isoelectric Point: 10.1709
VIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAKNGGNVKEVMDQALEEYIRKYLPDKL
Download Length: 216 bp
GTGATTGTGGGAAATTTAGGCGCACAAAAAGCAAAACGAAATGATACACCAATCAGTGCAAAAAAAGATATAATGGGAGA
TAAGACGGTTCGTGTTCGTGCTGACTTGCACCATATCATAAAAATCGAAACAGCAAAGAATGGCGGAAACGTAAAAGAAG
TTATGGATCAAGCCTTAGAAGAATATATACGGAAATATTTACCTGACAAACTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|---|---|---|---|
T6024 | Streptococcus pyogenes |
100 |
100 |
1 |
T6092 | Staphylococcus aureus |
100 |
100 |
1 |
T6104 | Enterococcus faecalis |
97.561 |
100 |
0.976 |
T6280 | Streptococcus suis 05ZYH33 |
43.75 |
86.822 |
0.38 |
T6037 | Enterococcus faecium |
40.167 |
93.359 |
0.375 |
T6080 | Streptococcus pneumoniae gamPNI0373 |
43.318 |
85.771 |
0.372 |
T6282 | Streptococcus pneumoniae R6 |
42.593 |
85.375 |
0.364 |
T10019 | Streptococcus pneumoniae TIGR4 |
42.13 |
85.375 |
0.36 |
Multiple sequence alignment
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|---|---|---|---|
AT6024 | Streptococcus pyogenes |
100 |
100 |
1 |
AT6092 | Staphylococcus aureus |
100 |
100 |
1 |
AT6104 | Enterococcus faecalis |
100 |
100 |
1 |
Multiple sequence alignment
Structures
Toxin
Source | ID | Structure |
---|---|---|
PDB | 1GVN | |
PDB | 3Q8X |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A829F0A3 |
References
(1) Urszula Zielenkiewicz et al. (2005) The toxin-antitoxin system of the streptococcal plasmid pSM19035. Journal of Bacteriology 187(17):6094-105. [PubMed:16109951]
(2) Ana G Camacho et al. (2002) In vitro and in vivo stability of the epsilon2zeta2 protein complex of the broad host-range Streptococcus pyogenes pSM19035 addiction system. Biological Chemistry 383(11):1701-13. [PubMed:12530535]
(3) Virginia S Lioy et al. (2006) pSM19035-encoded zeta toxin induces stasis followed by death in a subpopulation of cells. Microbiology (Reading, England) 152(Pt 8):2365-2379. [PubMed:16849801]
(4) Virginia S Lioy et al. (2010) Plasmid pSM19035, a model to study stable maintenance in Firmicutes. Plasmid 64(1):1-17. [PubMed:20403380]
(5) A Meinhart et al. (2001) Crystallization and preliminary X-ray diffraction studies of the epsilonzeta addiction system encoded by Streptococcus pyogenes plasmid pSM19035. Acta Crystallographica. Section D, Biological Crystallography 57(Pt 5):745-7. [PubMed:11320325]
(6) Anton Meinhart et al. (2003) Crystal structure of the plasmid maintenance system epsilon/zeta: functional mechanism of toxin zeta and inactivation by epsilon 2 zeta 2 complex formation. Proceedings of The National Academy of Sciences of The United States of America 100(4):1661-6. [PubMed:12571357]
(7) Virginia S Lioy et al. (2010) A toxin-antitoxin module as a target for antimicrobial development. Plasmid 63(1):31-9. [PubMed:19800365]
(8) Urszula Zielenkiewicz et al. (2009) In vivo interactions between toxin-antitoxin proteins epsilon and zeta of streptococcal plasmid pSM19035 in Saccharomyces cerevisiae. Journal of Bacteriology 191(11):3677-84. [PubMed:19346303]
(9) Iwona Brzozowska et al. (2014) The ClpXP protease is responsible for the degradation of the Epsilon antidote to the Zeta toxin of the streptococcal pSM19035 plasmid. The Journal of Biological Chemistry 289(11):7514-23. [PubMed:24492616]
(10) Iwona Brzozowska et al. (2012) Functioning of the TA cassette of streptococcal plasmid pSM19035 in various Gram-positive bacteria. Plasmid 68(1):51-60. [PubMed:22309878]
(11) Andrea Volante et al. (2014) The Interplay between Different Stability Systems Contributes to Faithful Segregation: Streptococcus pyogenes pSM19035 as a Model. Microbiology Spectrum 2(4):PLAS-0007-2013. [PubMed:26104212]
experimental literature
protein structure literature