10019

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) pezAT/-
Location 988052..989289 Replicon chromosome
Accession NC_003028
Organism Streptococcus pneumoniae TIGR4

Toxin (Protein)


Gene name pezT Uniprot ID Q97QZ1
Locus tag SP_1051 Protein ID WP_000405360.1
Coordinates 988528..989289 (+) Length 254 a.a.

Antitoxin (Protein)


Gene name pezA Uniprot ID -
Locus tag SP_1050 Protein ID WP_000579608.1
Coordinates 988052..988528 (+) Length 159 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SP_RS05170 (SP_1044) 983124..983978 + 855 WP_000900015.1 ADP-ribosylglycohydrolase family protein -
SP_RS05175 (SP_1045) 984090..984974 + 885 WP_000710115.1 diacylglycerol kinase family lipid kinase -
SP_RS12090 985138..985500 + 363 WP_000423476.1 alpha amylase N-terminal ig-like domain-containing protein -
SP_RS05180 (SP_1046) 985481..986877 + 1397 Protein_973 glycoside hydrolase family 13 protein -
SP_RS05185 (SP_1047) 986963..987280 + 318 WP_000995815.1 hypothetical protein -
SP_RS05190 (SP_1048) 987326..987607 + 282 WP_000266234.1 hypothetical protein -
SP_RS05195 (SP_1050) 988052..988528 + 477 WP_000579608.1 helix-turn-helix domain-containing protein Antitoxin
SP_RS05200 (SP_1051) 988528..989289 + 762 WP_000405360.1 toxin PezT Toxin
SP_RS05205 (SP_1052) 989317..990473 + 1157 Protein_978 hypothetical protein -
SP_RS05215 (SP_1054) 991304..991660 + 357 WP_000817623.1 hypothetical protein -
SP_RS05220 (SP_1055) 991665..992030 + 366 WP_000401239.1 MobC family plasmid mobilization relaxosome protein -
SP_RS05225 (SP_1056) 992017..993840 + 1824 WP_000237143.1 relaxase/mobilization nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 968351..1022298 53947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(25-215)

Antitoxin

Cro/C1-type helix-turn-helix domain

(3-60)


Sequences

Toxin        


Download         Length: 254 a.a.        Molecular weight: 29129.20 Da        Isoelectric Point: 7.5087

>T10019 WP_000405360.1 NC_003028:988528-989289 [Streptococcus pneumoniae TIGR4]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK

Download         Length: 762 bp

>T10019 NC_003028:988528-989289 [Streptococcus pneumoniae TIGR4]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTTGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGTGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGACAGTGTAGAATATACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGAGATACAATCTTTTGATAGAGGGAACTTTACGAACAGTTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTGGCCTTAATTGCGACAAAGCCTGAATTGTCGTATCTAAGTACTCTTATCCGTTATGAAGAA
CTGTACATTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
CACACGAAAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTACTCTTTGGGGAGTGGAGTCAGGTAGAGAAGGAGATGTTG
CAGGTGGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin


Download         Length: 159 a.a.        Molecular weight: 18247.70 Da        Isoelectric Point: 4.4188

>AT10019 WP_000579608.1 NC_003028:988052-988528 [Streptococcus pneumoniae TIGR4]
MIGKNIKSLRKTHDLTQLEFARIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Download         Length: 477 bp

>AT10019 NC_003028:988052-988528 [Streptococcus pneumoniae TIGR4]
ATGATTGGAAAGAACATAAAATCCTTGCGTAAAACACATGACTTAACACAACTCGAATTTGCACGGATTGTAGGTATTTC
ACGAAATAGTCTGAGTCGTTATGAAAATGGAACGAGTTCAGTCTCTACCGAATTAATAGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTAAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
GTCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGATTTGATTCATACGAATATCTATCTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAACGGATGGTGGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6282 Streptococcus pneumoniae R6

99.209

100

0.992
T6080 Streptococcus pneumoniae gamPNI0373

98.024

100

0.98
T6280 Streptococcus suis 05ZYH33

68.526

99.209

0.68
T6037 Enterococcus faecium

68.127

99.209

0.676
T6024 Streptococcus pyogenes

42.13

85.375

0.36
T6079 Streptococcus pyogenes

42.13

85.375

0.36
T6104 Enterococcus faecalis

42.13

85.375

0.36
T6092 Staphylococcus aureus

42.13

85.375

0.36

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT6080 Streptococcus pneumoniae gamPNI0373

98.734

100

0.987
AT6282 Streptococcus pneumoniae R6

98.734

100

0.987
AT6037 Enterococcus faecium

81.013

100

0.81
AT6280 Streptococcus suis 05ZYH33

81.013

100

0.81

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
PDB 2P5T

Antitoxin

Source ID Structure

References

(1) Seok Kooi Khoo et al. (2007) Molecular and structural characterization of the PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae. The Journal of Biological Chemistry 282(27):19606-18. [PubMed:17488720] 3D_structure

(2) Tiffany M Halvorsen et al. (2022) Comparison of Kill Switch Toxins in Plant-Beneficial Pseudomonas fluorescens Reveals Drivers of Lethality, Stability, and Escape. ACS Synthetic Biology 11(11):3785-3796. [PubMed:36346907]


3D_structure protein structure literature